About: Source identification of airborne Escherichia coli of swine house surroundings using ERIC-PCR and REP-PCR

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About: Source identification of airborne Escherichia coli of swine house surroundings using ERIC-PCR and REP-PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Source identification of airborne Escherichia coli of swine house surroundings using ERIC-PCR and REP-PCR
Creator	Wang, Yaling Cai, Yumei Chai, Tongjie Duan, Huiyong Gao, Jing Liu, Jianzhu Miao, Zengmin Qi, Chunhua Schlenker, Gerd Yao, Meiling Zhang, Xingxiao
Source	Elsevier; Medline; PMC
abstract	Abstract Evidence is mounting that microorganisms originating from livestock impact the air quality of the animal houses themselves and the public in the surrounding neighborhoods. The aim of this study was to develop efficient bacterial source tracking capabilities to identify sources of Escherichia coli aerosol pollution caused by pigs. Airborne E. coli were isolated from indoor air, upwind air (10 and 50m away) and downwind air samples (10, 50, 100, 200 and 400m away) for five swine houses using six-stage Andersen microbial samplers and Reuter-Centrifugal samplers (RCS). E. coli strains from pig fecal samples were also collected simultaneously. The enterobacterial repetitive intergenic consensus polymerize chain reaction (ERIC-PCR) and the repetitive extragenic palindromic (REP-PCR) approaches were used to study the genetic variability and to determine the strain relationships among E. coli isolated from different sites in each swine house. Results showed that 35.1% (20/57) of the bacterial DNA fingerprints from the fecal isolates matched with the corresponding strains isolated from indoor and downwind air samples (similarity ⩾90%). E. coli strains from the indoor and downwind air samples were closely related to the E. coli strains isolated from feces, while those isolated from upwind air samples (swine house C) had low similarity (61–69%). Our results suggest that some strains isolated from downwind and indoor air originated in the swine feces. Effective hygienic measures should be taken in animal farms to prevent or minimize the downwind spread of microorganism aerosol.
has issue date	2009-07-31(xsd:dateTime)
bibo:doi	10.1016/j.envres.2009.02.014
bibo:pmid	19349045
has license	els-covid
sha1sum (hex)	916d45569ec3b63c19a0ee9be8de36f502993a46
schema:url	https://doi.org/10.1016/j.envres.2009.02.014
resource representing a document's title	Source identification of airborne Escherichia coli of swine house surroundings using ERIC-PCR and REP-PCR
has PubMed Central identifier	PMC7126843
has PubMed identifier	19349045
schema:publication	Environmental Research
resource representing a document's body	covid:916d45569ec3b63c19a0ee9be8de36f502993a46#body_text
is schema:about of	named entity 'AIRBORNE' named entity 'ESCHERICHIA COLI' named entity 'CHAIN REACTION' named entity 'SURROUNDING' named entity 'SIMULTANEOUSLY' named entity '50%' named entity '200' named entity 'TO PREVENT' named entity 'TRACKING' named entity 'SITES' named entity 'PCR' named entity 'HOUSE' named entity 'DIFFERENT' named entity 'MOUNTING' named entity 'BACTERIAL DNA' named entity 'REPETITIVE' named entity 'INDOOR' named entity 'RELATIONSHIPS' named entity 'GENETIC VARIABILITY' named entity 'LIVESTOCK' named entity 'STRAINS' named entity 'ANIMAL' named entity 'PUBLIC' named entity 'SPREAD' named entity 'USING' named entity 'strain' named entity 'microorganism' named entity 'samplers' named entity 'aerosol' named entity 'similarity' named entity 'bacterial' named entity 'samples' named entity 'Source' named entity 'SWINE' named entity 'CAUSED BY' named entity 'TO IDENTIFY' named entity 'STUDY' named entity 'NEIGHBORHOODS' named entity 'E. COLI' named entity 'HOUSE' named entity 'SOURCE' named entity 'ERIC-PCR' named entity 'MICROORGANISMS' named entity 'STAGE' named entity 'ISOLATES' named entity 'DNA FINGERPRINTS' named entity 'SAMPLES' named entity 'AIM' named entity 'BACTERIAL' named entity 'EFFICIENT' named entity 'PIGS' named entity 'MATCHED' named entity 'ISOLATED' named entity 'USED' named entity 'DETERMINE' named entity 'IDENTIFICATION' named entity 'SURROUNDINGS' named entity 'REP-PCR' named entity 'USING' named entity 'STRAIN' named entity '100%' named entity 'MICROORGANISM' named entity 'FECAL' named entity 'SWINE' named entity 'ESCHERICHIA COLI' named entity 'FECES' named entity 'SUGGEST' named entity 'AIR QUALITY' named entity 'TAKEN' named entity 'OUR' named entity 'AIR'

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