About: Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L

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About: Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L
Creator	Pöhlmann, Stefan Hoffmann, Markus Hoenen, Thomas Müller, Andreas González-Hernández, Mariana
Source	Elsevier; Medline; PMC
abstract	Abstract Priming of the viral glycoprotein (GP) by the cellular proteases cathepsin B and L (CatB, CatL) is believed to be essential for cell entry of filoviruses. However, pseudotyping systems that predominantly produce non-filamentous particles have frequently been used to prove this concept. Here, we report that GP-mediated entry of retroviral-, rhabdoviral and filoviral particles depends on CatB/CatL activity and that this effect is cell line-independent. Moreover, we show that the human cell line Calu-3, which expresses low amounts of CatL, is largely resistant to entry driven by diverse filovirus GPs. Finally, we demonstrate that Calu-3 cell entry mediated by certain filovirus GPs can be rescued upon directed expression of CatL or DC-SIGN. Our results identify Calu-3 cells as largely resistant to filovirus GP-driven entry and demonstrate that entry is limited at the stage of virion attachment and GP priming.
has issue date	2019-06-30(xsd:dateTime)
bibo:doi	10.1016/j.virol.2019.03.020
bibo:pmid	30999160
has license	els-covid
sha1sum (hex)	915ed394319d501c10f529dba531cbd12a599d4a
schema:url	https://doi.org/10.1016/j.virol.2019.03.020
resource representing a document's title	Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L
has PubMed Central identifier	PMC7112014
has PubMed identifier	30999160
schema:publication	Virology
resource representing a document's body	covid:915ed394319d501c10f529dba531cbd12a599d4a#body_text
is schema:about of	named entity 'RESISTANT TO' named entity 'demonstrate' named entity 'concept' named entity 'believed' named entity 'expresses' named entity 'driven' named entity 'cells' named entity 'filovirus' named entity 'expression' named entity 'LOW' named entity 'DRIVEN' named entity 'DC-SIGN' named entity 'PRODUCE' named entity 'ENTRY' named entity 'PROTEASES' named entity 'ESSENTIAL' named entity 'CALU-3' named entity 'LARGELY' named entity 'MEDIATED' named entity 'DIRECTED' named entity 'VIRAL GLYCOPROTEIN' named entity 'FILOVIRUS' named entity 'CATHEPSIN B' named entity 'CATB' named entity 'DIRECTED' named entity 'EFFECT' named entity 'CERTAIN' named entity 'VIRION ATTACHMENT' named entity 'ACTIVITY' named entity 'SYSTEMS' named entity 'CATL' named entity 'PARTICLES' named entity 'FILOVIRUSES' named entity 'DIVERSE' named entity 'CALU-3 CELL' named entity 'RESISTANT TO' named entity 'CELLS' named entity 'CONCEPT' named entity 'FILAMENTOUS' named entity 'NON-' named entity 'CELLS' named entity 'HUMAN CELL LINE' named entity 'IDENTIFY' named entity 'GPS' named entity 'EXPRESSION' named entity 'USED' named entity 'RESULTS' named entity 'OUR' named entity 'HAVE' named entity 'LIMITED' named entity 'LARGELY' named entity 'FILOVIRUS' named entity 'GLYCOPROTEINS' named entity 'CALU-3' named entity 'ENTRY' named entity 'DC-SIGN' named entity 'EXPRESSION' named entity 'STAGE' named entity 'HERE' named entity 'CELL' named entity 'PRIMING' named entity 'AMOUNTS' named entity 'FREQUENTLY' named entity 'CELLULAR' named entity 'RETROVIRAL' named entity 'REPORT' named entity 'DRIVEN' named entity 'DEFECT' named entity 'CATHEPSIN L' named entity 'filovirus' named entity 'glycoprotein' named entity 'viral' named entity 'cell' named entity 'GPs' named entity 'cells' named entity 'demonstrate' named entity 'cell'

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