About: Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia
Creator	Mat-Rahim, Nor-Aziyah Suppiah, Jeyanthi Thayan, Ravindran Yusof, Apandi Rashid, Tengku Rogayah, Tengku Sa'at, Zainah
source	Elsevier; Medline; PMC
abstract	Abstract Objective To describe the complete nucleocapsid (N) gene region of Middle East respiratory syndrome coronavirus (MERS-CoV) from imported case in Malaysia and the relations with human- and camel-derived MERS-CoV. Methods Combination of throat and nasal swab specimens was subjected to viral RNA extraction. For screening, the extracted RNA was subjected to real-time RT-PCR targeting upstream of E gene, open reading frame 1b and open reading frame 1a. For confirmation, the RNA was subjected to RT-PCR targeting partial part of the RNA-dependent RNA polymerase and nucleocapsid, followed by amplification of complete N gene region. Nucleotide sequencing of the first Malaysian case of MERS-CoV was performed following the confirmation with real-time RT-PCR detection. Results Initial analysis of partial RNA-dependent RNA polymerase and N gene revealed that the nucleotides had high similarity to Jeddah_1_2013 strain. Analysis of complete N gene region (1 242 nucleotides) from the case showed high similarity and yet distinct to the nucleotide sequences of camel-derived MERS-CoV. Conclusions From the finding, there are possibilities that the patient acquired the infection from zoonotic transmission from dromedary camels.
has issue date	2015-07-31(xsd:dateTime)
bibo:doi	10.1016/s2222-1808(15)60833-7
bibo:pmid	32289031
has license	els-covid
sha1sum (hex)	91518c8dd2557ee864fc6cc0debcaf20899a457d
schema:url	https://doi.org/10.1016/s2222-1808%2815%2960833-7
resource representing a document's title	Nucleocapsid gene analysis from an imported case of Middle East respiratory syndrome coronavirus, Malaysia
has PubMed Central identifier	PMC7129196
has PubMed identifier	32289031
schema:publication	Asian Pacific Journal of Tropical Disease
resource representing a document's body	covid:91518c8dd2557ee864fc6cc0debcaf20899a457d#body_text
is schema:about of	named entity 'ASIAN' named entity 'MALAYSIA' named entity 'TROPICAL DISEASE' named entity 'CASE' named entity 'JOURNAL ' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'GENE ANALYSIS' named entity 'NUCLEOCAPSID' named entity 'IMPORTED' named entity 'ROUND' named entity 'PERFORMED' covid:arg/91518c8dd2557ee864fc6cc0debcaf20899a457d named entity 'Journal' named entity 'Middle East respiratory syndrome coronavirus' named entity 'Malaysia' named entity 'RNA' named entity 'camel' named entity 'RT-PCR' named entity 'RNA-dependent RNA polymerase' named entity 'RT-PCR' named entity 'diabetes' named entity 'MERS-CoV' named entity 'dromedary camels' named entity 'gene' named entity 'RT-PCR' named entity 'Oman' named entity 'phylogenetic analysis' named entity 'gene' named entity 'camel' named entity 'gene' named entity 'nostril' named entity 'cough' named entity 'virus' named entity 'MERS-CoV' named entity 'renal failure' named entity 'nucleocapsid' named entity 'nucleocapsid' named entity 'respiratory illnesses' named entity 'raw milk' named entity 'gene' named entity 'camel milk' named entity 'mode of transmission' named entity 'MERS-CoV' named entity 'MERS-CoV' named entity 'nucleocapsid' named entity 'infection' named entity 'reservoir hosts' named entity 'shortness of breath' named entity 'MERS-CoV' named entity 'unpasteurized' named entity 'camel' named entity 'primer' named entity 'RdRp' named entity 'Arabian Peninsula' named entity 'fever' named entity 'camel' named entity 'nucleocapsid' named entity 'cross-species transmission' named entity 'Malaysia' named entity 'Betacoronavirus' named entity 'MERS-CoV' named entity 'camel' named entity 'virus' named entity 'nucleocapsid' named entity 'camel' named entity 'MERS-CoV' named entity 'RdRp' named entity 'MERS-CoV' named entity 'Qatar' named entity 'rRT-PCR' named entity 'MERS-CoV' named entity 'Invitrogen' named entity 'camel' named entity 'gene' named entity 'Saudi Arabia'

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