About: In vitro Inhibition of Porcine Hemagglutinating Encephalomyelitis Virus Replication with siRNAs Targeting the Spike Glycoprotein and Replicase Polyprotein Genes

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About: In vitro Inhibition of Porcine Hemagglutinating Encephalomyelitis Virus Replication with siRNAs Targeting the Spike Glycoprotein and Replicase Polyprotein Genes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	In vitro Inhibition of Porcine Hemagglutinating Encephalomyelitis Virus Replication with siRNAs Targeting the Spike Glycoprotein and Replicase Polyprotein Genes
Creator	Wang, Gaili Chen, Keyan Gao, Feng He, Wenqi Lan, Yungang Lu, Huijun Song, Deguang Zhao, Kui
source	Medline; PMC
abstract	OBJECTIVE: The specific effect of RNA interference on the replication of porcine hemagglutinating encephalomyelitis virus (PHE-CoV) was explored. METHODS: Four species of small interfering RNA (siRNA), targeting different regions of the PHE-CoV spike glycoprotein and replicase polyprotein genes, were prepared by in vitro transcription. After transfection of PK-15 cells with each of the siRNAs followed by infection with PHE-CoV, the cytopathic effect (CPE) was examined by phase-contrast microscope, and viral proliferation within cells was examined by indirect immunofluorescence microscopy, hemagglutination (HA) test, TCID(50) assay and real-time RT-PCR. RESULTS: Examination of CPE demonstrated that the four siRNAs were capable of protecting cells against PHE-CoV invasion with very high specificity and efficiency. At 48 h post-infection, only a few siRNA-treated cells were positive for viral antigen staining, whereas most untreated virus-infected cells were positive. Transfection with siRNAs also suppressed the production of infectious virus by up to 18- to 32-fold as assessed by a HA test and 93- to 494-fold as assessed by TCID(50) assay. Furthermore, treatment with siRNAs caused a 53-91% reduction in the viral genome copy number as assessed by real-time RT-PCR. CONCLUSION: These results suggested that the four species of siRNAs can efficiently inhibit PHE-CoV genome replication and infectious virus production.
has issue date	2011-03-03(xsd:dateTime)
bibo:doi	10.1159/000323523
bibo:pmid	21372550
has license	no-cc
sha1sum (hex)	90695778d85ce96665b76564554d0eae7bb1a360
schema:url	https://doi.org/10.1159/000323523
resource representing a document's title	In vitro Inhibition of Porcine Hemagglutinating Encephalomyelitis Virus Replication with siRNAs Targeting the Spike Glycoprotein and Replicase Polyprotein Genes
has PubMed Central identifier	PMC7179546
has PubMed identifier	21372550
schema:publication	Intervirology
resource representing a document's body	covid:90695778d85ce96665b76564554d0eae7bb1a360#body_text
is schema:about of	named entity 'copy number' named entity 'species' named entity 'assessed' named entity 'viral genome' named entity 'PHE' named entity 'Fax' named entity 'reagent' named entity 'infectious disease' named entity 'serum' named entity 'RNAi' named entity 'TGEV' named entity 'physiological' named entity 'viral replication' named entity 'supernatants' named entity 'gene' named entity 'siRNA' named entity 'PHE' named entity 'serum' named entity 'coronavirus' named entity 'virions' named entity 'hemagglutinating' named entity 'infection' named entity 'GenBank' named entity 'Open reading frames' named entity 'transcription' named entity 'Agarose Gel' named entity 'gene' named entity 'hemagglutinating' named entity 'sequence identity' named entity 'nucleocapsid' named entity 'Qiagen' named entity 'ribosomal frameshifting' named entity 'antiviral therapies' named entity 'China' named entity 'siRNAs' named entity 'PHE' named entity 'siRNA' named entity 'classical swine fever virus' named entity 'receptor' named entity 'transfection' named entity 'PCR' named entity 'homology' named entity 'highly conserved' named entity 'positive test' named entity 'serum' named entity 'siRNA' named entity 'transcription' named entity 'titer' named entity 'Nerve cells' named entity 'antiviral therapy' named entity 'negative control' named entity 'transfected' named entity 'RNA' named entity 'siRNA' named entity 'PHE' named entity 'PHE' named entity 'transfection' named entity 'mRNAs' named entity 'PHE' named entity 'coronaviruses' named entity 'genome replication' named entity 'infection' named entity 'GenBank' named entity 'siRNA' named entity 'PHE' named entity 'transfected' named entity 'protein' named entity 'porcine reproductive and respiratory syndrome virus' named entity 'siRNA' named entity 'copy number' named entity 'viral RNAs' named entity 'pig' named entity 'plasmid' named entity 'PHE'

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