About: Propagation of Rhinovirus C in Differentiated Immortalized Human Airway HBEC3-KT Epithelial Cells

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About: Propagation of Rhinovirus C in Differentiated Immortalized Human Airway HBEC3-KT Epithelial Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Propagation of Rhinovirus C in Differentiated Immortalized Human Airway HBEC3-KT Epithelial Cells
Creator	Kageyama, Tsutomu Saito, Shinji Takayama, Ikuyo Nagata, Noriyo Kaida, Atsushi Kubo, Hideyuki Odagiri, Takato Nakauchi, Mina Oba, Kunihiro
Source	PMC
abstract	Rhinoviruses (RVs) are classified into three species: RV-A, B, and C. Unlike RV-A and -B, RV-C cannot be propagated using standard cell culture systems. In order to isolate RV-Cs from clinical specimens and gain a better understanding of their biological properties and pathogenesis, we established air–liquid-interface (ALI) culture methods using HBEC3-KT and HSAEC1-KT immortalized human airway epithelial cells. HBEC3- and HSAEC1-ALI cultures morphologically resembled pseudostratified epithelia with cilia and goblet cells. Two fully sequenced clinical RV-C isolates, RV-C9 and -C53, were propagated in HBEC3-ALI cultures, and increases in viral RNA ranging from 1.71 log(10) to 7.06 log(10) copies were observed. However, this propagation did not occur in HSAEC1-ALI cultures. Using the HBEC3-ALI culture system, 11 clinical strains of RV-C were isolated from 23 clinical specimens, and of them, nine were passaged and re-propagated. The 11 clinical isolates were classified as RV-C2, -C6, -C9, -C12, -C18, -C23, -C40, and -C53 types according to their VP1 sequences. Our stable HBEC3-ALI culture system is the first cultivable cell model that supports the growth of multiple RV-C virus types from clinical specimens. Thus, the HBEC3-ALI culture system provides a cheap and easy-to-use alternative to existing cell models for isolating and investigating RV-Cs.
has issue date	2019-03-04(xsd:dateTime)
bibo:doi	10.3390/v11030216
bibo:pmid	30836639
has license	cc-by
sha1sum (hex)	905078cd289a214842aa5101ae3351d89a0046bf
schema:url	https://doi.org/10.3390/v11030216
resource representing a document's title	Propagation of Rhinovirus C in Differentiated Immortalized Human Airway HBEC3-KT Epithelial Cells
has PubMed Central identifier	PMC6466094
has PubMed identifier	30836639
schema:publication	Viruses
resource representing a document's body	covid:905078cd289a214842aa5101ae3351d89a0046bf#body_text
is schema:about of	named entity 'propagated' named entity 'C18' named entity 'culture' named entity 'specimens' named entity 'isolating' named entity 'observed' named entity 'Airway' covid:arg/905078cd289a214842aa5101ae3351d89a0046bf named entity 'RVs' named entity 'propagation' named entity 'ranging' named entity 'models' named entity 'RV-C' named entity 'RV-C' named entity 'RV-C' named entity 'morphologically' named entity 'VP1' named entity 'Rhinovirus C' named entity 'passaged' named entity 'multiple comparisons' named entity 'Ribonuclease' named entity 'Thermo Fisher Scientific' named entity 'RV-C' named entity 'RV-C' named entity 'RNA' named entity 'RNA' named entity 'culture methods' named entity 'minimum essential medium' named entity 'epithelial' named entity 'real-time RT-PCR' named entity 'pseudostratified' named entity 'viruses' named entity 'collagen' named entity 'replication mechanisms' named entity 'respiratory diseases' named entity '1, 2' named entity 'inoculation' named entity 'RNA genomes' named entity 'RNA' named entity 'copy numbers' named entity 'UTR' named entity 'Sanger sequencing' named entity 'upper airway' named entity 'next-generation sequencing' named entity 'RNA' named entity 'upper respiratory tract infections' named entity 'cell morphology' named entity 'Whole-genome sequences' named entity 'encapsidated' named entity 'RNA' named entity 'ATCC' named entity 'RNA extraction' named entity 'RNA' named entity 'RNA' named entity 'RNA' named entity 'RNA' named entity 'RNA' named entity 'detection limit' named entity 'real-time RT-PCR' named entity 'NTPs' named entity 'inoculation' named entity 'intercellular adhesion molecule 1' named entity 'viruses' named entity 'RNA' named entity 'RNA' named entity 'S1C' named entity 'morphological differences' named entity 'RNA' named entity 'Reagents' named entity 'STEMCELL Technologies' named entity 'Ipswich, MA' named entity 'viral genomes' named entity 'RV-C'

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