About: Antibody response of definitive hosts against antigens of two life stages of the neuropathogenic schistosome Trichobilharzia regenti

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Antibody response of definitive hosts against antigens of two life stages of the neuropathogenic schistosome Trichobilharzia regenti Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Antibody response of definitive hosts against antigens of two life stages of the neuropathogenic schistosome Trichobilharzia regenti
Creator	Horák, Petr Mikeš, Libor Pecková, Monika Turjanicová, Libuše
source	Medline; PMC
abstract	BACKGROUND: The nasal avian schistosome Trichobilharzia regenti spends part of its intravertebrate period of life within the central nervous system. Migration of the parasites can be accompanied by neuromotor disorders or paralysis in natural definitive hosts (ducks) and even in laboratory mammals. Cercariae are also able to penetrate human skin and induce cercarial dermatitis. While the cellular and antibody responses against cercariae and migrating schistosomula have been investigated in mice, little is known about immune reactions in birds. This study first describes the dynamics of antibody response in infected ducks and identifies frequently recognized antigens that may serve as diagnostic markers of infection by T. regenti. METHODS: Groups of 35 domestic ducks and 10 mallards were exposed to different doses of T. regenti cercariae. Sera were collected at predefined time intervals and tested by ELISA for the presence of specific anti-cercarial IgY and IgM. Antigens recognized by the antibodies were identified on Western blots of cercariae and schistosomula. The applicability in immunodiagnostics was statistically evaluated by expression of specificity and sensitivity values for individual antigens. RESULTS: In ELISA, the levels of anti-cercarial IgM peaked on day 15 pi. Increased production of IgY associated with the later phases of infection was observed in most individuals around 20 dpi and culminated 30 dpi. The time course of antibody response did not differ among experimental groups, variations were only observed in the levels of specific IgY which depended rather on the age of ducks at the time of infection than on the infectious dose. On Western blots, 40 cercarial and 7 schistosomular antigens were recognized by IgY from infected ducks. Among them, 4 cercarial antigens of 50, 47, 32 and 19 kDa provided the most sensitive and specific reactions. CONCLUSIONS: Antigens of cercariae and schistosomula elicited distinct antibody response in ducks, which correlated positively with the age of animals at the time of infection. Several antigens originating in cercariae and fewer in schistosomula were recognized by IgY with diverse sensitivity and specificity; only a few seemed to be common to both stages. Four of them were considered as the most promising candidates for immunodiagnostics. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-1007-y) contains supplementary material, which is available to authorized users.
has issue date	2015-07-28(xsd:dateTime)
bibo:doi	10.1186/s13071-015-1007-y
bibo:pmid	26216102
has license	cc-by
sha1sum (hex)	902feae616d8f0ffb508ef3a4a1fbc344bb63133
schema:url	https://doi.org/10.1186/s13071-015-1007-y
resource representing a document's title	Antibody response of definitive hosts against antigens of two life stages of the neuropathogenic schistosome Trichobilharzia regenti
has PubMed Central identifier	PMC4517386
has PubMed identifier	26216102
schema:publication	Parasit Vectors
resource representing a document's body	covid:902feae616d8f0ffb508ef3a4a1fbc344bb63133#body_text
is schema:about of	named entity 'TRICHOBILHARZIA REGENTI' named entity 'TIME COURSE' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'CERCARIAE' named entity 'CERCARIAL DERMATITIS' named entity 'INDIVIDUAL' named entity 'MIGRATION' named entity 'STUDY' named entity 'SPECIFICITY' named entity 'VARIATIONS' named entity 'TIME' named entity 'DISORDERS' named entity 'HAVE' named entity 'PRODUCTION' named entity 'COMMON' named entity 'KNOWN' named entity 'DIAGNOSTIC MARKERS' named entity 'NATURAL' named entity 'ANTIGENS' named entity 'LITTLE' named entity 'INFECTED' named entity 'SPECIFIC' named entity 'DOMESTIC' named entity 'STAGES' named entity 'DYNAMICS' named entity 'STATISTICALLY' named entity 'LATER' named entity 'EXPRESSION' named entity 'AGE' named entity 'CELLULAR' named entity 'INTERVALS' named entity 'IGY' named entity 'DEFINITIVE' named entity 'SPENDS' named entity 'DUCKS' named entity 'REACTIONS' named entity 'DOSES' named entity 'MAMMALS' named entity 'ANTIBODY RESPONSE' named entity 'DIFFERENT' named entity 'PENETRATE' named entity 'INFECTION' named entity 'EXPOSED TO' named entity 'BACKGROUND' named entity 'GROUPS' named entity 'SENSITIVE' named entity 'MICE' named entity 'PARALYSIS' named entity 'INFECTIOUS DOSE' named entity 'IGM' named entity 'CORRELATED' named entity 'SENSITIVITY' named entity '50%' named entity 'DAY' named entity 'RESULTS' named entity 'BIRDS' named entity 'CONSIDERED' named entity 'ANTIBODIES' named entity 'ELICITED' named entity 'IMMUNODIAGNOSTICS' named entity 'CENTRAL NERVOUS SYSTEM' named entity 'ABOUT' named entity 'HOSTS' named entity 'ELISA' named entity 'ANTIGENS' named entity 'DEFINITIVE' named entity 'ANTIBODY RESPONSE' named entity 'LIFE STAGES' named entity 'SCHISTOSOME' named entity 'FREQUENTLY' named entity 'LEVELS' named entity 'KDA' named entity 'IDENTIFIED' named entity 'VALUES' named entity 'AVIAN SCHISTOSOME' named entity 'ITS' named entity 'MIGRATING' named entity 'DIVERSE'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software