About: A rapid detection method for Vaccinia virus, the surrogate for smallpox virus

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About: A rapid detection method for Vaccinia virus, the surrogate for smallpox virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A rapid detection method for Vaccinia virus, the surrogate for smallpox virus
Creator	Donaldson, Kim Kramer, Marianne Lim, Daniel
Source	Elsevier; Medline; PMC
abstract	Abstract Prior to the World Health Organization’s announcement of total eradication in 1977 [J. Am. Med. Assoc. 281 (1999) 1735], smallpox was a worldwide pathogen. Vaccinations were ceased in 1980 and now with a largely unprotected world population, smallpox is considered the ideal biowarfare agent [Antiviral Res. 57 (2002) 1]. Infection normally occurs after implantation of the virus on the oropharyngeal or respiratory mucosa [J. Am. Med. Assoc. 281 (1999) 2127]. Smallpox virus can be detected from the throats of exposed individuals prior to onset of illness and prior to the infectious stage of the illness. A rapid, sensitive real-time assay to detect Variola virus (smallpox) has been developed using the Vaccinia virus, a surrogate of smallpox, as a target. Cyanine 5 dye-labeled anti-Vaccinia antibody was used in a sandwich immunoassay to produce a fluorescent signal in the presence of the Vaccinia virus. The signal was detected using the Analyte 2000 biosensor (Research International, Monroe, WA). The Analyte 2000 uses a 635nm laser diode to provide excitation light that is launched into a polystyrene optical waveguide. Fluorescent molecules within the evanescent wave are excited and a portion of their emission energy recouples into the waveguide. A photodiode quantifies the emission light at wavelengths between 670 and 710nm. The biosensor was able to detect a minimum of 2.5×105 pfu/ml of Vaccinia virus in seeded throat culture swab specimens.
has issue date	2004-09-15(xsd:dateTime)
bibo:doi	10.1016/j.bios.2004.01.029
bibo:pmid	15308237
has license	els-covid
sha1sum (hex)	8faf766a36e21ff43d3e3fec82247b34efdf2f44
schema:url	https://doi.org/10.1016/j.bios.2004.01.029
resource representing a document's title	A rapid detection method for Vaccinia virus, the surrogate for smallpox virus
has PubMed Central identifier	PMC7125742
has PubMed identifier	15308237
schema:publication	Biosensors and Bioelectronics
resource representing a document's body	covid:8faf766a36e21ff43d3e3fec82247b34efdf2f44#body_text
is schema:about of	named entity 'SMALLPOX VIRUS' named entity 'sandwich' named entity 'specimens' named entity 'biosensor' named entity 'wavelengths' named entity 'POPULATION' named entity 'SWAB' named entity 'BIOSENSOR' named entity 'OPTICAL' named entity 'IMPLANTATION' named entity 'MED' named entity 'ANTIBODY' named entity 'LASER DIODE' named entity 'EXCITATION' named entity '710' named entity 'LABELED' named entity 'ILLNESS' named entity 'PORTION' named entity 'TOTAL' named entity 'SANDWICH IMMUNOASSAY' named entity 'RESPIRATORY MUCOSA' named entity 'USED' covid:arg/8faf766a36e21ff43d3e3fec82247b34efdf2f44 named entity 'DETECTION METHOD' named entity 'RAPID' named entity 'SURROGATE' named entity 'WAVEGUIDE' named entity 'LIGHT' named entity 'WORLDWIDE' named entity 'TO DETECT' named entity 'PFU' named entity 'ANALYTE' named entity 'AGENT' named entity 'THROAT CULTURE' named entity 'VACCINIA VIRUS' named entity 'IDEAL' named entity 'CYANINE' named entity '670' named entity 'SMALLPOX' named entity 'ASSOC' named entity 'CONSIDERED' named entity 'VACCINATIONS' named entity 'EXPOSED' named entity 'MONROE' named entity 'DYE' named entity 'POLYSTYRENE' named entity 'REAL-TIME' named entity 'PRIOR TO' named entity 'PHOTODIODE' named entity 'RAPID' named entity 'RES' named entity 'WORLD' named entity 'VACCINIA' named entity 'ENERGY' named entity 'DETECTED' named entity 'VACCINIA VIRUS' named entity 'BIOWARFARE' named entity 'PATHOGEN' named entity 'USING' named entity 'SURROGATE' named entity 'MINIMUM' named entity 'VARIOLA VIRUS' named entity 'TO PRODUCE' named entity 'FLUORESCENT' named entity 'EXCITED' named entity 'SMALLPOX VIRUS' named entity 'ANNOUNCEMENT' named entity 'TO PROVIDE' named entity 'SENSITIVE' named entity 'MOLECULES' named entity 'EMISSION' named entity 'STAGE' named entity 'INDIVIDUALS' named entity '27S' named entity 'TARGET' named entity 'WORLD HEALTH ORGANIZATION' named entity 'ERADICATION' named entity 'ONSET OF ILLNESS' named entity 'WAVELENGTHS'

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