About: The non-structural protein Nsp10 of mouse hepatitis virus binds zinc ions and nucleic acids

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: The non-structural protein Nsp10 of mouse hepatitis virus binds zinc ions and nucleic acids Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The non-structural protein Nsp10 of mouse hepatitis virus binds zinc ions and nucleic acids
Creator	Hilgenfeld, Rolf Canard, Bruno Coutard, Bruno Snijder, Eric Matthes, Nele Moll, Ralf Mesters, Jeroen
Source	Elsevier; Medline; PMC
abstract	The non-structural protein Nsp10 of coronaviruses is a small cleavage product of the viral replicase polyprotein that has been implicated in RNA synthesis. Nsp10 of mouse hepatitis virus (MHV) displays an apparent molecular mass of 13–16 kDa in reducing SDS–PAGE and analytical gel filtration, while dynamic light scattering suggests the existence of oligomeric forms. Atomic absorption spectroscopy reveals two metal ions per Nsp10 monomer, with a preference for Zn(2+) over Fe(2+/3+) and Co(2+). These are probably bound by two Zn-finger-like motifs. Moreover, MHV Nsp10 interacts with tRNA, single-stranded RNA, double-stranded DNA and, to a lesser extent, single-stranded DNA as shown by gel-shift experiments. The K(d) for tRNA is 2.1 ± 0.2 μM.
has issue date	2006-07-24(xsd:dateTime)
bibo:doi	10.1016/j.febslet.2006.06.061
bibo:pmid	16828088
has license	no-cc
sha1sum (hex)	8ee9b35c304d8a2f3f088ff0e4dbe1e94bff79b2
schema:url	https://doi.org/10.1016/j.febslet.2006.06.061
resource representing a document's title	The non-structural protein Nsp10 of mouse hepatitis virus binds zinc ions and nucleic acids
has PubMed Central identifier	PMC7094219
has PubMed identifier	16828088
schema:publication	FEBS Lett
resource representing a document's body	covid:8ee9b35c304d8a2f3f088ff0e4dbe1e94bff79b2#body_text
is schema:about of	named entity 'BINDS' named entity 'ZINC IONS' named entity 'reveals' named entity 'bound' named entity 'nucleic acids' named entity 'NSP10' named entity 'NUCLEIC ACIDS' named entity 'NON-' named entity 'CORONAVIRUSES' named entity 'PROBABLY' named entity 'DYNAMIC LIGHT SCATTERING' named entity 'KDA' named entity 'PREFERENCE' named entity 'SDS-PAGE' named entity 'EXTENT' named entity 'PRODUCT' named entity '0.2' named entity 'PROTEIN ' named entity 'MOLECULAR MASS' named entity 'MOTIFS' covid:arg/8ee9b35c304d8a2f3f088ff0e4dbe1e94bff79b2 named entity 'ANALYTICAL' named entity 'ATOMIC ABSORPTION SPECTROSCOPY ' named entity '2.1' named entity 'DISPLAYS' named entity 'REVEALS' named entity 'CLEAVAGE' named entity 'DNA' named entity 'FORMS' named entity 'MOUSE HEPATITIS VIRUS' named entity 'RNA SYNTHESIS' named entity 'SMALL' named entity 'MONOMER' named entity 'APPARENT' named entity 'STRUCTURAL PROTEIN' named entity 'FINGER' named entity 'MOUSE HEPATITIS VIRUS' named entity 'LESSER' named entity 'REPLICASE' named entity 'POLYPROTEIN' named entity 'BOUND' named entity 'GEL' named entity 'THESE' named entity 'REDUCING' named entity 'SINGLE-STRANDED RNA' named entity 'DOUBLE-STRANDED DNA' named entity 'STRUCTURAL PROTEIN' named entity 'METAL IONS' named entity 'GEL FILTRATION' named entity 'EXPERIMENTS' named entity 'SHIFT' named entity 'LIKE' named entity 'TRNA' named entity 'VIRAL' named entity 'IS A' named entity 'PROTEIN ' named entity 'NON-' named entity 'viral' named entity 'dynamic light scattering' named entity 'extent' named entity 'reducing' named entity 'protein' named entity 'existence' named entity 'binds' named entity 'mouse hepatitis virus' named entity 'gel filtration' named entity 'coronaviruses' named entity 'dynamic light scattering' named entity 'MHV' named entity 'zinc ions' named entity 'affinity column' named entity 'monomer' named entity 'molecular mass' named entity 'single-stranded' named entity 'zinc fingers'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software