About: The YXXΦ motif within the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is crucial for its intracellular transport

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About: The YXXΦ motif within the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is crucial for its intracellular transport Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The YXXΦ motif within the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is crucial for its intracellular transport
Creator	Minakshi, Rinki Padhan, Kartika
source	Medline; PMC
abstract	BACKGROUND: The SARS coronavirus (SARS-CoV) 3a protein functions as an ion channel, induces apoptosis and is important for viral pathogenesis. It is expressed on the cell surface and contains a tyrosine-based sorting motif and a di-acidic motif, which may be crucial for its intracellular trafficking. However the role of these motifs is not fully understood in the case of 3a protein. METHODS: The subcellular distribution of the 3a protein was studied by immunofluorescence staining of cells transfected with wild type and mutant constructs along with markers for different intracellular compartments. Semi-quantitative RT-PCR was performed to estimate the mRNA where as western blotting was carried out to detect protein levels of wild type and mutant 3a proteins. In vitro transcription- translation was performed to estimate cell free protein synthesis. RESULTS: While the wild type 3a protein is efficiently transported to the plasma membrane, the protein with mutations in the tyrosine and valine residues within the YXXV motif (ΔYXXΦ) accumulated in the Golgi compartment. However the 3a protein with mutations within the EXD di-acidic motif (ΔEXD) showed an intracellular distribution similar to the wild type protein. Increased retention of the ΔYXXΦ protein in the Golgi compartment also increased its association with lipid droplets. The ΔYXXΦ protein also expressed at significantly lower levels compared to the wild type 3a protein, which was reversed with Brefeldin A and Aprotinin. CONCLUSIONS: The data suggest that the YXXΦ motif of the SARS-CoV 3a protein is necessary for Golgi to plasma membrane transport, in the absence of which the protein is targeted to lysosomal degradation compartment via lipid droplets.
has issue date	2014-04-24(xsd:dateTime)
bibo:doi	10.1186/1743-422x-11-75
bibo:pmid	24762043
has license	cc-by
sha1sum (hex)	8eb469fc4a148699ffdf0969e3ea75048412472d
schema:url	https://doi.org/10.1186/1743-422x-11-75
resource representing a document's title	The YXXΦ motif within the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein is crucial for its intracellular transport
has PubMed Central identifier	PMC4004515
has PubMed identifier	24762043
schema:publication	Virol J
resource representing a document's body	covid:8eb469fc4a148699ffdf0969e3ea75048412472d#body_text
is schema:about of	covid:arg/8eb469fc4a148699ffdf0969e3ea75048412472d named entity 'MOTIFS' named entity 'BACKGROUND' named entity 'APOPTOSIS' named entity 'INTRACELLULAR' named entity 'MAY BE' named entity 'IMPORTANT' named entity 'SARS CORONAVIRUS' named entity 'CELL SURFACE' named entity 'TYROSINE' named entity 'CONTAINS' named entity 'MOTIF' named entity 'PROTEIN' named entity 'MOTIF' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'ITS' named entity 'VIRAL PATHOGENESIS' named entity 'EXPRESSED' named entity 'TRAFFICKING' named entity 'BASED' named entity 'ION CHANNEL' named entity 'UNDERSTOOD' named entity 'PROTEIN ' named entity 'SORTING' named entity 'FULLY' named entity 'ITS' named entity 'PROTEIN ' named entity 'INTRACELLULAR TRANSPORT' named entity 'FUNCTIONS' named entity 'CASE' named entity 'THESE' named entity 'ACIDIC' named entity 'ROLE' named entity 'THE CELL' named entity 'induces' named entity 'ion' named entity 'cell' named entity 'SARS coronavirus' named entity 'motif' named entity 'motif' named entity 'cell surface' named entity 'protein' named entity 'severe acute respiratory syndrome coronavirus' named entity 'DNA sequencing' named entity 'Lipid droplets' named entity 'Golgi' named entity 'colocalization' named entity 'motif' named entity 'antibodies' named entity 'antibodies' named entity 'cell surface' named entity 'YFP' named entity 'dye' named entity 'SARS-CoV' named entity 'protein' named entity 'lipids' named entity 'transfection' named entity 'Golgi' named entity 'protein' named entity 'beta Coronavirus' named entity 'T7 promoter' named entity 'transcription' named entity 'virus' named entity 'Golgi' named entity 'cytoplasmic domain' named entity 'eukaryotic' named entity 'lysing' named entity 'staining' named entity 'ARF' named entity 'Myc-tag' named entity 'intracellular' named entity 'lysosomal' named entity 'Over-expressed' named entity 'Golgi' named entity 'lysosomal degradation' named entity 'plasma membrane' named entity 'tyrosine' named entity 'host cell' named entity 'Golgi' named entity 'CD3'

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