About: Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection

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About: Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection
Creator	Wang, Wei Zhang, Jing Deubel, Vincent Lin, Xin Wang, Yongjin Delpeyroux, Francis Ren, Peijun Sheng, Jun Yan, Huajie Bessaud, Mael Huang, Ting
Source	PMC
abstract	BACKGROUND: Human rhinoviruses (HRVs) are a highly prevalent cause of acute respiratory infection in children. They are classified into at least three species, HRV-A, HRV-B and HRV-C, which are characterized by sequencing the 5′ untranslated region (UTR) or the VP4/VP2 region of the genome. Given the increased interest for novel HRV strain identification and their worldwide distribution, we have carried out clinical and molecular diagnosis of HRV strains in a 2-year study of children with acute respiratory infection visiting one district hospital in Shanghai. METHODOLOGY/FINDINGS: We cloned and sequenced a 924-nt fragment that covered part of the 5′UTR and the VP4/VP2 capsid genes. Sixty-four HRV-infected outpatients were diagnosed amongst 827 children with acute low respiratory tract infection. Two samples were co-infected with HRV-A and HRV-B or HRV-C. By comparative analysis of the VP4/VP2 sequences of the 66 HRVs, we showed a high diversity of strains in HRV-A and HRV-B species, and a prevalence of 51.5% of strains that belonged to the recently identified HRV-C species. When analyzing a fragment of the 5′ UTR, we characterized at least two subspecies of HRV-C: HRV-Cc, which clustered differently from HRV-A and HRV-B, and HRV-Ca, which resulted from previous recombination in this region with sequences related to HRV-A. The full-length sequence of one strain of each HRV-Ca and HRV-Cc subspecies was obtained for comparative analysis. We confirmed the close relationship of their structural proteins but showed apparent additional recombination events in the 2A gene and 3′UTR of the HRV-Ca strain. Double or triple infections with HRV-C and respiratory syncytial virus and/or bocavirus were diagnosed in 33.3% of the HRV-infected patients, but no correlation with severity of clinical outcome was observed. CONCLUSION: Our study showed a high diversity of HRV strains that cause bronchitis and pneumonia in children. A predominance of HRV-C over HRV-A and HRV-B was observed, and two subspecies of HRV-C were identified, the diversity of which seemed to be related to recombination with former HRV-A strains. None of the HRV-C strains appeared to have a higher clinical impact than HRV-A or HRV-B on respiratory compromise.
has issue date	2009-07-27(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0006355
bibo:pmid	19633719
has license	cc-by
sha1sum (hex)	8d095d0275e474dbb9d9b63a75591ff2c0667d73
schema:url	https://doi.org/10.1371/journal.pone.0006355
resource representing a document's title	Evidence of Recombination and Genetic Diversity in Human Rhinoviruses in Children with Acute Respiratory Infection
has PubMed Central identifier	PMC2712091
has PubMed identifier	19633719
schema:publication	PLoS One
resource representing a document's body	covid:8d095d0275e474dbb9d9b63a75591ff2c0667d73#body_text
is schema:about of	named entity 'Given' named entity 'acute respiratory infection' named entity 'Diversity' named entity 'CHILDREN' named entity 'HAVE' named entity 'INCREASED' named entity 'DISTRIBUTION' named entity 'HRV' named entity 'CLASSIFIED' covid:arg/8d095d0275e474dbb9d9b63a75591ff2c0667d73 named entity 'UNTRANSLATED REGION' named entity 'DISTRICT HOSPITAL' named entity 'PREVALENT' named entity 'INTEREST' named entity 'HUMAN' named entity 'IDENTIFICATION' named entity 'THEIR' named entity 'BACKGROUND' named entity 'HUMAN' named entity 'EVIDENCE OF' named entity 'ACUTE RESPIRATORY INFECTION' named entity 'GENETIC DIVERSITY' named entity 'RECOMBINATION' named entity 'ACUTE RESPIRATORY INFECTION' named entity 'CHARACTERIZED' named entity 'VP4' named entity 'YEAR' named entity 'HIGHLY' named entity 'GENOME' named entity 'CAUSE' named entity 'SPECIES' named entity 'STRAINS' named entity 'MOLECULAR DIAGNOSIS' named entity 'NOVEL' named entity 'WORLDWIDE' named entity 'REGION' named entity 'HRV-A' named entity 'CLINICAL' named entity 'CHILDREN' named entity 'SEQUENCING' named entity 'RHINOVIRUSES' named entity 'STUDY' named entity 'RHINOVIRUSES' named entity 'STRAIN' named entity 'GIVEN' named entity 'CARRIED' named entity 'carried' named entity 'classified' named entity 'sequencing' named entity 'acute respiratory infection' named entity 'untranslated region' named entity 'HRV' named entity 'HRV' named entity 'HRV' named entity 'Recombination' named entity 'HRV' named entity 'viruses' named entity 'gene analysis' named entity 'recombinant' named entity 'phylogenetic analysis' named entity 'HRV' named entity 'HRV' named entity 'HRV' named entity 'HRV' named entity 'subspecies' named entity 'bronchiolitis' named entity 'HRV' named entity 'Nested PCR' named entity 'untranslated region' named entity 'HRV' named entity 'HRV'

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