About: Pathogenicity and immunogenicity of attenuated porcine epidemic diarrhea virus PC22A strain in conventional weaned pigs

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About: Pathogenicity and immunogenicity of attenuated porcine epidemic diarrhea virus PC22A strain in conventional weaned pigs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Pathogenicity and immunogenicity of attenuated porcine epidemic diarrhea virus PC22A strain in conventional weaned pigs
Creator	Lin, Chun-Ming Wang, Qiuhong Hou, Yixuan Vlasova, Anastasia Saif, Linda Boley, Patricia Ghimire, Shristi Langel, Stephanie
Source	Medline; PMC
abstract	BACKGROUND: Porcine epidemic diarrhea virus (PEDV) causes diarrhea in all ages of pigs with 50–100% mortality rates in neonatal piglets. In the United States, inactivated and subunit PEDV vaccines for pregnant sows are available, but fail to induce sufficient protection in neonatal piglets farrowed from PEDV naïve sows. A safe and efficacious live attenuated vaccine that can prime mucosal immune responses is urgently needed. In this study, we evaluated the safety and efficacy of two attenuated PEDV vaccine candidates, the emerging non-S INDEL PEDV strain PC22A at the 100th cell culture passage level - Clone no. 4 (P100C4) and at the 120th passage level (P120), in weaned pigs. RESULTS: Four groups of 40-day-old weaned pigs were inoculated orally with PEDV PC22A-P3 (virulent), -P100C4, -P120, and mock, respectively, and challenged with the P3 virus at 24 days post-inoculation (dpi). After inoculation, P3 caused diarrhea in all pigs with a high level of fecal viral RNA shedding. P100C4 and P120 did not cause diarrhea in pigs, although viral RNA was detected in feces of all pigs, except for one P100C4-inoculated pig. Compared with the P120 group, P3- and P100C4-inoculated pigs had higher serum PEDV-specific IgG and viral neutralizing (VN) antibody (Ab) titers at 14 dpi. After the challenge, no pigs in the P3 group but all pigs in the P100C4, P120, and mock groups had diarrhea. Compared with the P120 group, pigs in the P100C4 group had a more rapid decline in fecal PEDV RNA shedding titers, higher titers of serum PEDV-specific IgG, IgA, and VN Abs, and higher numbers of intestinal IgA Ab-secreting cells. CONCLUSIONS: PEDV PC22A P100C4 and P120 were fully attenuated in weaned pigs but failed to elicit protection against virulent P3 challenge. P100C4 induced higher PEDV-specific antibody responses than P120 post inoculation resulting in a greater anamnestic response post challenge. Therefore, P100C4 potentially could be tested as a priming vaccine or be further modified using reverse genetics. It also can be administered in multiple doses or be combined with inactivated or subunit vaccines and adjuvants as a PEDV vaccination regimen, whose efficacy can be tested in the future. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-018-1756-x) contains supplementary material, which is available to authorized users.
has issue date	2019-01-11(xsd:dateTime)
bibo:doi	10.1186/s12917-018-1756-x
bibo:pmid	30634958
has license	cc-by
sha1sum (hex)	8cef766849d0d8ad7a5ff33d7f66299de03cf583
schema:url	https://doi.org/10.1186/s12917-018-1756-x
resource representing a document's title	Pathogenicity and immunogenicity of attenuated porcine epidemic diarrhea virus PC22A strain in conventional weaned pigs
has PubMed Central identifier	PMC6329175
has PubMed identifier	30634958
schema:publication	BMC Vet Res
resource representing a document's body	covid:8cef766849d0d8ad7a5ff33d7f66299de03cf583#body_text
is schema:about of	named entity 'rapid' named entity 'viral' named entity 'United States' named entity 'P120' named entity 'P120' named entity 'attenuated' named entity 'diarrhea' named entity 'orally' named entity 'efficacious' named entity 'pigs' named entity 'efficacy' named entity 'attenuated' named entity 'LIVE ATTENUATED VACCINE' named entity 'DAY-OLD' named entity 'RNA' named entity 'MOCK' named entity 'EXCEPT FOR' named entity 'INOCULATION' named entity 'ATTENUATED' named entity 'CELLS' named entity 'STUDY' named entity 'FECAL' named entity 'VACCINE' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'ALL AGES' named entity 'IGG' named entity 'RESULTS' named entity 'INDEL' named entity 'SERUM' named entity 'NEONATAL' named entity 'CAUSE' named entity 'INTESTINAL' named entity 'PRIME' named entity 'PASSAGE' named entity 'CLONE' named entity 'SOWS' named entity 'INDUCE' named entity 'CONVENTIONAL' named entity 'VIRUS' named entity '100%' named entity 'RAPID DECLINE' named entity 'DID' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'DPI' named entity 'STRAIN' named entity 'PATHOGENICITY' named entity 'CELL CULTURE' named entity 'STRAIN' named entity 'MUCOSAL' named entity 'ORALLY' named entity 'EVALUATED' named entity 'PREGNANT' named entity 'IGA AB' named entity 'CHALLENGE' named entity 'DAYS' named entity 'HIGHER' named entity 'IGA' named entity 'VIRAL RNA' named entity 'MORTALITY RATES' named entity 'VACCINES' named entity 'SAFE' named entity 'SPECIFIC' named entity 'WEANED' named entity 'BACKGROUND' named entity 'SUBUNIT' named entity 'GROUPS' named entity 'FECES' named entity 'NON-' named entity 'VIRAL' named entity 'INACTIVATED' named entity 'UNITED STATES' named entity 'GROUP' named entity 'COMPARED' named entity 'SHEDDING'

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