About: Migrating a lab-developed MERS-CoV real-time PCR to 3 “Sample to Result” systems: experiences on optimization and validation

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About: Migrating a lab-developed MERS-CoV real-time PCR to 3 “Sample to Result” systems: experiences on optimization and validation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Migrating a lab-developed MERS-CoV real-time PCR to 3 “Sample to Result” systems: experiences on optimization and validation
Creator	Beuselinck, Kurt Desmet, Stefanie Frans, Glynis Lagrou, Katrien Peeters, Bart Saegeman, Veroniek Ranst, Marc
Source	Elsevier; Medline; PMC
abstract	Abstract The goal of the study was to adapt our Middle East respiratory syndrome coronavirus (MERS-CoV) lab-developed test (LDT) to 3 “Sample to Result” (S2R) systems: BD MAX (BD), ELITe InGenius (ELITechGroup), and ARIES (Luminex). The BD MAX and InGenius system allowed use of lab-developed primers and TaqMan probes, while ARIES required conversion to MultiCode primers for melting curve analysis. Each device required ≤1 day of training and assay optimization. No discordant results were noted after analysis of 32 External Quality Control (EQC) samples. On a 10-fold dilution series of a MERS-CoV–positive EQC sample, InGenius obtained the highest detection rate. Laboratory technicians rated the ARIES as the user-friendliest. It also required the least hands-on time. BD MAX had the lowest turnaround time and highest throughput. While each device had distinguishing system properties with associated (dis)advantages, the 3 S2R systems were comparable in terms of assay development and validation.
has issue date	2019-08-31(xsd:dateTime)
bibo:doi	10.1016/j.diagmicrobio.2019.02.006
bibo:pmid	30929995
has license	els-covid
sha1sum (hex)	8c7ca66668249f82b79e4873baf99bdb043c1c96
schema:url	https://doi.org/10.1016/j.diagmicrobio.2019.02.006
resource representing a document's title	Migrating a lab-developed MERS-CoV real-time PCR to 3 “Sample to Result” systems: experiences on optimization and validation
has PubMed Central identifier	PMC7127711
has PubMed identifier	30929995
schema:publication	Diagnostic Microbiology and Infectious Disease
resource representing a document's body	covid:8c7ca66668249f82b79e4873baf99bdb043c1c96#body_text
is schema:about of	named entity 'LDT' named entity 'training' named entity 'validation' named entity 'optimization' covid:arg/8c7ca66668249f82b79e4873baf99bdb043c1c96 named entity 'probes' named entity 'study' named entity 'validation' named entity 'terms' named entity 'Result' named entity 'TaqMan' named entity 'Luminex' named entity 'melting curve analysis' named entity 'InGenius' named entity 'primers' named entity 'Middle East respiratory syndrome coronavirus' named entity 'ARIES' named entity 'assay' named entity 'OC43' named entity 'PCR' named entity 'multiplex reactions' named entity 'PDV' named entity 'laboratory technicians' named entity 'MERS-CoV' named entity 'World Health Organization' named entity 'Asia' named entity 'Cy5' named entity 'MERS-CoV' named entity 'ARIES' named entity 'World Health Organization' named entity 'PCR' named entity 'false-positive' named entity 'PCR' named entity 'fluorometric' named entity 'PDV' named entity 'Hajj' named entity 'RT-PCR' named entity 'PDV' named entity 'Italy' named entity 'MERS-CoV' named entity '5′ end' named entity 'primers' named entity 'hemagglutinin' named entity 'statistical analysis' named entity 'primers' named entity 'primers' named entity 'reverse-transcription polymerase chain reaction' named entity 'OC43' named entity 'cryovials' named entity 'PCR' named entity 'BioMérieux' named entity 'fluorescent' named entity 'World Health Organization' named entity 'PCR' named entity 'PDV' named entity 'primers' named entity 'primers' named entity 'MERS-CoV' named entity 'Reagent' named entity 'Coronavirus' named entity 'PCR' named entity 'nucleic acid' named entity 'PCR' named entity 'PCR' named entity 'PCR' named entity 'North America' named entity 'primers' named entity 'PCR' named entity 'ARIES' named entity 'PCR amplification' named entity 'reagent' named entity 'molecular diagnostics' named entity 'MERS-CoV' named entity 'ARIES' named entity 'MERS-CoV'

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