About: Identification of Interactions between Sindbis Virus Capsid Protein and Cytoplasmic vRNA as Novel Virulence Determinants

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About: Identification of Interactions between Sindbis Virus Capsid Protein and Cytoplasmic vRNA as Novel Virulence Determinants Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Identification of Interactions between Sindbis Virus Capsid Protein and Cytoplasmic vRNA as Novel Virulence Determinants
Creator	Gebhart, Natasha Hardy, Richard Jones, Claire May, Nicholas Morrison, Thomas Nease, Lauren Sokoloski, Kevin
source	PMC
abstract	Alphaviruses are arthropod-borne viruses that represent a significant threat to public health at a global level. While the formation of alphaviral nucleocapsid cores, consisting of cargo nucleic acid and the viral capsid protein, is an essential molecular process of infection, the precise interactions between the two partners are ill-defined. A CLIP-seq approach was used to screen for candidate sites of interaction between the viral Capsid protein and genomic RNA of Sindbis virus (SINV), a model alphavirus. The data presented in this report indicates that the SINV capsid protein binds to specific viral RNA sequences in the cytoplasm of infected cells, but its interaction with genomic RNA in mature extracellular viral particles is largely non-specific in terms of nucleotide sequence. Mutational analyses of the cytoplasmic viral RNA-capsid interaction sites revealed a functional role for capsid binding early in infection. Interaction site mutants exhibited decreased viral growth kinetics; however, this defect was not a function of decreased particle production. Rather mutation of the cytoplasmic capsid-RNA interaction sites negatively affected the functional capacity of the incoming viral genomic RNAs leading to decreased infectivity. Furthermore, cytoplasmic capsid interaction site mutants are attenuated in a murine model of neurotropic alphavirus infection. Collectively, the findings of this study indicate that the identified cytoplasmic interactions of the viral capsid protein and genomic RNA, while not essential for particle formation, are necessary for genomic RNA function early during infection. This previously unappreciated role of capsid protein during the alphaviral replication cycle also constitutes a novel virulence determinant.
has issue date	2017-06-29(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1006473
bibo:pmid	28662211
has license	cc-by
sha1sum (hex)	8be33a516142d0fa91dd2355a5d41a5357895188
schema:url	https://doi.org/10.1371/journal.ppat.1006473
resource representing a document's title	Identification of Interactions between Sindbis Virus Capsid Protein and Cytoplasmic vRNA as Novel Virulence Determinants
has PubMed Central identifier	PMC5507600
has PubMed identifier	28662211
schema:publication	PLoS Pathog
resource representing a document's body	covid:8be33a516142d0fa91dd2355a5d41a5357895188#body_text
is schema:about of	named entity 'terms' named entity 'particles' named entity 'production' named entity 'CLIP-seq' named entity 'virus' named entity 'alphavirus' named entity 'global' named entity 'While' named entity 'protein' named entity 'mature' named entity 'viral capsid' named entity 'infection' named entity 'capsid' named entity 'essential' named entity 'Protein' covid:arg/8be33a516142d0fa91dd2355a5d41a5357895188 named entity 'early' named entity 'binds' named entity 'viral' named entity 'nucleocapsid' named entity 'protein' named entity 'viral' named entity 'leading' named entity 'revealed' named entity 'interaction' named entity 'cargo' named entity 'virulence' named entity 'formation' named entity 'interaction' named entity 'cytoplasmic' named entity 'capsid' named entity 'Sindbis' named entity 'Determinants' named entity 'virus' named entity 'cytoplasm' named entity 'Sindbis' named entity 'cytoplasmic' named entity 'RNA sequences' named entity 'mutants' named entity 'viral RNA' named entity 'viral particles' named entity 'virulence' named entity 'alphavirus' named entity 'RNA' named entity 'growth kinetics' named entity 'protein' named entity 'alphavirus' named entity 'capsid' named entity 'Sindbis Virus' named entity 'immunoprecipitation' named entity 'symptomology' named entity 'centrifugation' named entity 'BHK' named entity 'SINV' named entity 'RNA' named entity 'weight loss' named entity 'infection' named entity 'Coronaviridae' named entity 'SNPs' named entity 'cytoplasmic' named entity 'virus' named entity 'CLIP-Seq' named entity 'core protein' named entity 'viral adsorption' named entity 'RNA' named entity 'RNA' named entity 'dimerization' named entity 'SINV' named entity 'viral capsid' named entity 'mutation' named entity 'HEK293 cells' named entity 'cytoplasmic' named entity 'gene expression' named entity 'positive-sense RNA' named entity 'Capsid'

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