About: Lessons learned: Optimization of a murine small bowel resection model

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Lessons learned: Optimization of a murine small bowel resection model Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Lessons learned: Optimization of a murine small bowel resection model
Creator	Erwin, Christopher Guo, Jun Nair, Rajalakshmi Martin, Colin Taylor, Janice Warner, Brad
Source	Elsevier; Medline; PMC
abstract	BACKGROUND/PURPOSE: Central to the use of murine models of disease is the ability to derive reproducible data. The purpose of this study was to determine factors contributing to variability in our murine model of small bowel resection (SBR). METHODS: Male C57Bl/6 mice were randomized to sham or 50% SBR. The effect of housing type (pathogen-free versus standard housing), nutrition (reconstituted powder versus tube feeding formulation), and correlates of intestinal morphology with gene expression changes were investigated Multiple linear regression modeling or one-way ANOVA was used for data analysis. RESULTS: Pathogen-free mice had significantly shorter ileal villi at baseline and demonstrated greater villus growth after SBR compared to mice housed in standard rooms. Food type did not affect adaptation. Gene expression changes were more consistent and significant in isolated crypt cells that demonstrated adaptive growth when compared with crypts that did not deepen after SBR. CONCLUSION: Maintenance of mice in pathogen-free conditions and restricting gene expression analysis to individual animals exhibiting morphologic adaptation enhances sensitivity and specificity of data derived from this model. These refinements will minimize experimental variability and lead to improved understanding of the complex process of intestinal adaptation.
has issue date	2008-06-01(xsd:dateTime)
bibo:doi	10.1016/j.jpedsurg.2008.02.025
bibo:pmid	18558176
has license	green-oa
sha1sum (hex)	8b2d07f60391054a3a923959c8f842144436825c
schema:url	https://doi.org/10.1016/j.jpedsurg.2008.02.025
resource representing a document's title	Lessons learned: Optimization of a murine small bowel resection model
has PubMed Central identifier	PMC2481288
has PubMed identifier	18558176
schema:publication	Journal of Pediatric Surgery
resource representing a document's body	covid:8b2d07f60391054a3a923959c8f842144436825c#body_text
is schema:about of	named entity 'RESECTION MODEL' named entity 'The' named entity 'study' named entity 'intestinal' named entity 'nutrition' named entity 'correlates' named entity 'Lessons learned' named entity 'IMPROVED' named entity 'VARIABILITY' named entity 'ADAPTIVE' named entity 'ADAPTATION' named entity 'VILLUS' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'MURINE' named entity 'ISOLATED' named entity 'INVESTIGATED' named entity 'PURPOSE' named entity 'SIGNIFICANT' named entity 'ANIMALS' named entity 'DEEPEN' named entity 'DERIVED' named entity 'DISEASE' named entity 'GENE EXPRESSION ANALYSIS' named entity 'TUBE FEEDING' named entity 'SBR' named entity 'MODELS' named entity 'STUDY' named entity 'MULTIPLE' named entity 'SMALL BOWEL RESECTION' named entity 'OPTIMIZATION' named entity '50%' named entity 'CELLS' named entity 'DEMONSTRATED' named entity 'SMALL BOWEL RESECTION' named entity 'DATA ANALYSIS' named entity 'ANALYSIS OF VARIANCE' named entity 'EFFECT' named entity 'GENE EXPRESSION' named entity 'STANDARD' named entity 'BASELINE' named entity 'LINEAR REGRESSION' named entity 'LEARNED' named entity 'RESULTS' named entity 'EXHIBITING' named entity 'DID' named entity 'THESE' named entity 'HOUSED' named entity 'MODELING' named entity 'CONSISTENT' named entity 'ILEAL' named entity 'MURINE' named entity 'HOUSING' named entity 'INTESTINAL ADAPTATION' named entity 'CONDITIONS' named entity 'POWDER' named entity 'FORMULATION' named entity 'PATHOGEN' named entity 'CHANGES' named entity 'AFFECT' named entity 'INDIVIDUAL' named entity 'CRYPT' named entity 'CONCLUSION' named entity 'GROWTH' named entity 'COMPLEX' named entity 'MODEL' named entity 'MAINTENANCE' named entity 'CONTRIBUTING' named entity 'FREE' named entity 'GREATER' named entity 'CENTRAL' named entity 'SHAM' named entity 'EXPERIMENTAL' named entity 'USE OF' named entity 'DETERMINE' named entity 'LEAD' named entity 'HOUSING TYPE'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software