About: Recapitulation of SARS-CoV-2 Infection and Cholangiocyte Damage with Human Liver Organoids

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About: Recapitulation of SARS-CoV-2 Infection and Cholangiocyte Damage with Human Liver Organoids Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Recapitulation of SARS-CoV-2 Infection and Cholangiocyte Damage with Human Liver Organoids
Creator	Xie, Youhua Xu, Wei Yang, Li Wang, Yuyan Zhang, Rong Ni, Chao Zhao, Bing Gao, Ran Liang, Jianqing Liang, Junbo Lin, Xinhua Lv, Ting Wang, Xiaoyue Wei, Jinsong Zhang, Qisheng
Source	BioRxiv
abstract	The newly emerged pandemic coronavirus, SARS-CoV-2, has posed a significant public health threat worldwide. However, the mode of virus transmission and tissue tropism is not well established yet. Recent findings of substantial liver damage in patients and ACE2+ cholangiocytes in healthy liver tissues prompted us to hypothesize that human liver ductal organoids could serve as a model to determine the susceptibility and mechanisms underlining the liver damage upon SARS-CoV-2 infection. By single-cell RNA sequencing, we found that long-term liver ductal organoid culture preserved the human specific ACE2+ population of cholangiocytes. Moreover, human liver ductal organoids were permissive to SARS-CoV-2 infection and support robust replication. Notably, virus infection impaired the barrier and bile acid transporting functions of cholangiocytes through dysregulation of genes involved in tight junction formation and bile acid transportation, which could explain the bile acid accumulation and consequent liver damage in patients. These results indicate that control of liver damage caused directly by viral infection should be valued in treating COVID-19 patients. Our findings also provide an application of human organoids in investigating the tropism and pathogenesis of SARS-CoV-2, which would facilitate novel drug discovery.
has issue date	2020-03-17(xsd:dateTime)
bibo:doi	10.1101/2020.03.16.990317
has license	biorxiv
sha1sum (hex)	8abeefb10db504d0fbf74c081f8c0c11fea1cca1
schema:url	https://doi.org/10.1101/2020.03.16.990317
resource representing a document's title	Recapitulation of SARS-CoV-2 Infection and Cholangiocyte Damage with Human Liver Organoids
schema:publication	bioRxiv
resource representing a document's body	covid:8abeefb10db504d0fbf74c081f8c0c11fea1cca1#body_text
is schema:about of	named entity 'viral infection' named entity 'formation' named entity 'infection' named entity 'liver damage' named entity 'CELL' named entity 'MODE' named entity 'ductal' named entity 'single-cell' named entity 'organoids' named entity 'human' named entity 'barrier' named entity 'organoids' named entity 'transmission' named entity 'susceptibility' named entity 'SARS-CoV-2' named entity 'robust' named entity 'determine' named entity 'substantial' named entity 'liver' named entity 'patients' named entity 'Recapitulation' named entity 'Infection' named entity 'liver damage' named entity 'infection' named entity 'SARS-CoV-2' named entity 'organoids' named entity 'liver' named entity 'organoids' named entity 'RNA sequencing' named entity 'COVID-19' named entity 'cholangiocytes' named entity 'liver damage' named entity 'SARS-CoV-2' named entity 'virus transmission' named entity 'bile acid' named entity 'tight junction' named entity 'SARS-CoV-2' named entity 'organoid' named entity 'virus infection' named entity 'Student's t-test' named entity 'human liver' named entity 'organoids' named entity 'organ damage' named entity 'organoids' named entity 'human Liver' named entity 'China' named entity 'RNA sequencing' named entity 'ANOVA' named entity 'human liver' named entity 'liver damage' named entity 'infection' named entity 'scRNA-seq' named entity 'organoids' named entity 'organoids' named entity '2019-nCoV' named entity 'AST' named entity 'normal range' named entity 'transcriptome' named entity 'global public health' named entity 'cholangiocytes' named entity 'China' named entity 'cough' named entity 'organoid' named entity 'COVID' named entity 'human liver' named entity 'total bilirubin' named entity 'long-term' named entity 'liver function' named entity 'human liver' named entity 'SARS-CoV-2'

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