About: Different host cell proteases activate the SARS-coronavirus spike-protein for cell–cell and virus

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About: Different host cell proteases activate the SARS-coronavirus spike-protein for cell–cell and virus–cell fusion Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Different host cell proteases activate the SARS-coronavirus spike-protein for cell–cell and virus–cell fusion
Creator	Bertram, Stephanie Pöhlmann, Stefan Lu, Kai Simmons, Graham Glowacka, Ilona Hofmann, Heike Steffen, Imke Chaipan, Chawaree Bates, Paul Agudelo, Juliet Rennekamp, Andrew
Source	Elsevier; Medline; PMC
abstract	Abstract Severe acute respiratory syndrome coronavirus (SARS-CoV) poses a considerable threat to human health. Activation of the viral spike (S)-protein by host cell proteases is essential for viral infectivity. However, the cleavage sites in SARS-S and the protease(s) activating SARS-S are incompletely defined. We found that R667 was dispensable for SARS-S-driven virus–cell fusion and for SARS-S-activation by trypsin and cathepsin L in a virus–virus fusion assay. Mutation T760R, which optimizes the minimal furin consensus motif 758-RXXR-762, and furin overexpression augmented SARS-S activity, but did not result in detectable SARS-S cleavage. Finally, SARS-S-driven cell–cell fusion was independent of cathepsin L, a protease essential for virus–cell fusion. Instead, a so far unknown leupeptin-sensitive host cell protease activated cellular SARS-S for fusion with target cells expressing high levels of ACE2. Thus, different host cell proteases activate SARS-S for virus–cell and cell–cell fusion and SARS-S cleavage at R667 and 758-RXXR-762 can be dispensable for SARS-S activation.
has issue date	2011-05-10(xsd:dateTime)
bibo:doi	10.1016/j.virol.2011.02.020
bibo:pmid	21435673
has license	els-covid
sha1sum (hex)	8a7e52bd7fe2bd2db85f83a9c36530399f08d7a0
schema:url	https://doi.org/10.1016/j.virol.2011.02.020
resource representing a document's title	Different host cell proteases activate the SARS-coronavirus spike-protein for cell–cell and virus–cell fusion
has PubMed Central identifier	PMC3086175
has PubMed identifier	21435673
schema:publication	Virology
resource representing a document's body	covid:8a7e52bd7fe2bd2db85f83a9c36530399f08d7a0#body_text
is schema:about of	named entity 'fusion' named entity 'PROTEIN ' named entity 'INDEPENDENT OF' named entity 'ACTIVATE' named entity 'ACTIVATE' named entity 'SARS' named entity 'MOTIF' named entity 'MUTATION' named entity 'AUGMENTED' named entity 'ACE2' named entity '758' named entity 'HOST CELL' named entity 'LEVELS' named entity 'MINIMAL' named entity 'TRYPSIN' named entity 'VIRAL' named entity 'UNKNOWN' named entity 'OVEREXPRESSION' named entity 'CELL FUSION' named entity 'RESULT' named entity 'TARGET CELLS' named entity 'CONSIDERABLE' named entity 'HUMAN HEALTH' named entity '28S' named entity 'PROTEASES' named entity 'CELL FUSION' named entity 'VIRUS' named entity 'SPIKE' named entity 'CORONAVIRUS' named entity 'SARS' named entity 'CELL' named entity 'SENSITIVE' named entity 'CLEAVAGE' named entity 'ESSENTIAL' named entity 'VIRUS' named entity 'DEFINED' named entity 'ACTIVATED' named entity 'THREAT' named entity 'PROTEASES' named entity 'ACTIVITY' named entity 'SPIKE' named entity 'PROTEASE' named entity 'HOST CELL' named entity 'DIFFERENT' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'INFECTIVITY' named entity 'FAR' named entity 'LEUPEPTIN' named entity 'CELLULAR' named entity 'DRIVEN' named entity 'PROTEIN ' named entity 'HIGH' named entity '762' named entity 'BUT' named entity 'CATHEPSIN L' named entity 'FOUND' named entity 'CONSENSUS' named entity 'DETECTABLE' named entity 'DID' named entity 'ACTIVATING' named entity 'FUSION' named entity 'FURIN' named entity 'ASSAY' named entity 'DIFFERENT' named entity 'ACTIVATION' named entity 'SITES' named entity 'CELL' covid:arg/8a7e52bd7fe2bd2db85f83a9c36530399f08d7a0 named entity 'assay' named entity 'cell' named entity 'activity'

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