About: PCR Improves Diagnostic Yield from Lung Aspiration in Malawian Children with Radiologically Confirmed Pneumonia

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	PCR Improves Diagnostic Yield from Lung Aspiration in Malawian Children with Radiologically Confirmed Pneumonia
Creator	Guiver, Malcolm Denis, Brigitte Dove, Winifred Jeffers, Graham Anthony, C Banda, Daniel Carrol, Enitan Graham, Stephen Mankhambo, Limangeni Molyneux, Elizabeth Molyneux, Malcolm
source	Medline; PMC
abstract	BACKGROUND: Accurate data on childhood pneumonia aetiology are essential especially from regions where mortality is high, in order to inform case-management guidelines and the potential of prevention strategies such as bacterial conjugate vaccines. Yield from blood culture is low, but lung aspirate culture provides a higher diagnostic yield. We aimed to determine if diagnostic yield could be increased further by polymerase chain reaction (PCR) detection of bacteria (Streptococcus pneumoniae and Haemophilus influenzae b) and viruses in lung aspirate fluid. METHODS: A total of 95 children with radiological focal, lobar or segmental consolidation had lung aspirate performed and sent for bacterial culture and for PCR for detection of bacteria, viruses and Pneumocystis jirovecii. In children with a pneumococcal aetiology, pneumococcal bacterial loads were calculated in blood and lung aspirate fluid. RESULTS: Blood culture identified a bacterial pathogen in only 8 patients (8%). With the addition of PCR on lung aspirate samples, causative pathogens (bacterial, viral, pneumocystis) were identified singly or as co-infections in 59 children (62%). The commonest bacterial organism was S.pneumoniae (41%), followed by H. influenzae b (6%), and the commonest virus identified was adenovirus (16%), followed by human bocavirus (HBoV) (4%), either as single or co-infection. CONCLUSIONS: In a select group of African children, lung aspirate PCR significantly improves diagnostic yield. Our study confirms a major role of S.pneumoniae and viruses in the aetiology of childhood pneumonia in Africa.
has issue date	2011-06-14(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0021042
bibo:pmid	21695128
has license	cc-by
sha1sum (hex)	8866d2274d3ecb0ef50ccf806d9e03839ad2edbc
schema:url	https://doi.org/10.1371/journal.pone.0021042
resource representing a document's title	PCR Improves Diagnostic Yield from Lung Aspiration in Malawian Children with Radiologically Confirmed Pneumonia
has PubMed Central identifier	PMC3114850
has PubMed identifier	21695128
schema:publication	PLoS One
resource representing a document's body	covid:8866d2274d3ecb0ef50ccf806d9e03839ad2edbc#body_text
is schema:about of	named entity 'PNEUMONIA' named entity 'Yield' named entity 'Aspiration' named entity 'CASE' named entity 'DETECTION OF BACTERIA' named entity 'PREVENTION STRATEGIES' named entity 'LOW' named entity 'WHERE' named entity 'FLUID' named entity 'ACCURATE' named entity 'GUIDELINES ' named entity 'CULTURE' named entity 'DIAGNOSTIC YIELD' named entity 'ORDER' named entity 'CHILDREN' named entity 'PROVIDES' named entity 'HIGH' named entity 'AETIOLOGY' named entity 'POLYMERASE CHAIN REACTION' named entity 'YIELD' named entity 'POTENTIAL' named entity 'MALAWIAN' named entity 'DIAGNOSTIC YIELD' named entity 'DETERMINE' named entity 'IMPROVES' named entity 'PCR' named entity 'LUNG ASPIRATION' named entity 'TO INFORM' named entity 'VIRUSES' named entity 'LUNG' named entity 'ESSENTIAL' named entity 'BACTERIA ' named entity 'ASPIRATE' named entity 'CONJUGATE VACCINES' named entity 'DATA' named entity 'MORTALITY' named entity 'MANAGEMENT' named entity 'CHILDHOOD PNEUMONIA' named entity 'BACTERIAL' named entity 'CONFIRMED' named entity 'BLOOD CULTURE' named entity 'INCREASED' named entity 'PNEUMONIAE' named entity 'REGIONS' named entity 'BACKGROUND' named entity 'BUT' named entity 'COULD BE' named entity 'HAEMOPHILUS INFLUENZAE B' covid:arg/8866d2274d3ecb0ef50ccf806d9e03839ad2edbc named entity 'aspirate' named entity 'conjugate vaccines' named entity 'Yield' named entity 'Haemophilus influenzae' named entity 'viruses' named entity 'blood culture' named entity 'lung aspirate' named entity 'Streptococcus pneumoniae' named entity 'PCR' named entity 'Lung' named entity 'Radiologically' named entity 'lung aspirate' named entity 'blood culture' named entity 'WHO' named entity 'auscultation' named entity 'PCR' named entity 'pneumococcal' named entity 'PcP' named entity 'PcP' named entity 'bacterial pneumonia' named entity 'pneumococcal conjugate vaccine' named entity 'Mycobacterium tuberculosis'

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