About: EGFR as a Negative Regulatory Protein Adjusts the Activity and Mobility of NHE3 in the Cell Membrane of IPEC-J2 Cells With TGEV Infection

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About: EGFR as a Negative Regulatory Protein Adjusts the Activity and Mobility of NHE3 in the Cell Membrane of IPEC-J2 Cells With TGEV Infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	EGFR as a Negative Regulatory Protein Adjusts the Activity and Mobility of NHE3 in the Cell Membrane of IPEC-J2 Cells With TGEV Infection
Creator	Wang, Xiuqing Liu, Jia Yang, Yang Sun, Dongbo Wang, Kai An, Ping Huang, Shilei Ran, Ling Song, Zhenhui Xie, Luyi Yang, Zhou Yuan, Peng
Source	Medline; PMC
abstract	Transmissible gastroenteritis (TGE) has caused devastating economic losses to the swine industry worldwide, despite extensive research focusing on the pathogenesis of virus infection. The molecular pathogenic mechanism of TGEV-induced diarrhea in piglets is unknown. Intestinal diarrhea is closely related to the function of the Na(+)/H(+) exchanger protein NHE3 in the brush border membrane of small intestine epithelial cells. The epidermal growth factor receptor (EGFR) may act to regulate NHE3 expression. In addition, EGFR may promote viral invasion of host cells. The present study aimed to determine whether NHE3 activity is regulated by altering EGFR expression to affect Na(+) absorption in TGEV-infected intestinal epithelial cells. Porcine intestinal epithelial cells were used as models for TGEV infection. The results showed that Na(+) absorption and NHE3 expression levels decreased in TGEV-infected cells. Proliferation of TGEV within IPEC-J2 cells could be inhibited by treatment with the EGFR inhibitor AG1478 and knockdown; resulting in recovery of Na(+) absorption in TGEV infected cells and increasing the activity and expression of NHE3. Moreover, we demonstrated that NHE3 activity was regulated through the EGFR/ERK pathway. Importantly, NHE3 mobility on the plasma membrane of TGEV infected cells was significantly weaker than that in normal cells, and EGFR inhibition and knockdown recovered this mobility. Our research indicated that NHE3 activity was negatively regulated by EGFR in TGEV-infected intestinal epithelial cells.
has issue date	2018-11-13(xsd:dateTime)
bibo:doi	10.3389/fmicb.2018.02734
bibo:pmid	30483239
has license	cc-by
sha1sum (hex)	8863943c0c9153a3677761f16e704854c65c3783
schema:url	https://doi.org/10.3389/fmicb.2018.02734
resource representing a document's title	EGFR as a Negative Regulatory Protein Adjusts the Activity and Mobility of NHE3 in the Cell Membrane of IPEC-J2 Cells With TGEV Infection
has PubMed Central identifier	PMC6243134
has PubMed identifier	30483239
schema:publication	Front Microbiol
resource representing a document's body	covid:8863943c0c9153a3677761f16e704854c65c3783#body_text
is schema:about of	named entity 'INFECTION' named entity 'growth factor' named entity 'pathogenesis' named entity 'receptor' named entity 'study' named entity 'EGFR' named entity 'THE CELL' named entity 'ACTIVITY' named entity 'STUDY' named entity 'PATHOGENESIS' named entity 'HOST CELLS' named entity 'TGEV' named entity 'SMALL INTESTINE' named entity 'REGULATED' named entity 'CAUSED' named entity 'INDUSTRY' named entity 'PORCINE' named entity 'RESULTING IN' named entity 'RESULTS' named entity 'PLASMA MEMBRANE' named entity 'DETERMINE' named entity 'INFECTED' named entity 'MECHANISM' named entity 'UNKNOWN' named entity 'GASTROENTERITIS' named entity 'LEVELS' named entity 'VIRAL' named entity 'MOBILITY' named entity 'INDUCED' named entity 'PATHOGENIC' named entity 'FOCUSING' named entity 'PRESENT' named entity 'INVASION OF HOST' named entity 'DIARRHEA' named entity 'PROMOTE' named entity 'RECOVERED' named entity 'PROLIFERATION' named entity 'AFFECT' named entity 'VIRUS INFECTION' named entity 'INHIBITED' named entity 'ACTIVITY' named entity 'INCREASING' named entity 'INHIBITION' named entity 'ACT' named entity 'DECREASED' named entity 'MODELS' named entity 'SWINE' named entity 'BRUSH BORDER MEMBRANE' named entity 'NHE3' named entity 'REGULATORY PROTEIN' named entity 'PROTEIN ' named entity 'ABSORPTION' named entity 'CELLS' named entity 'MOBILITY' named entity 'EGFR' named entity 'TGEV' named entity 'RECOVERY' named entity 'TO REGULATE' named entity 'PROTEIN ' named entity 'MOLECULAR' named entity 'DEMONSTRATED' named entity 'COULD BE' named entity 'EPIDERMAL GROWTH FACTOR RECEPTOR' named entity 'RESEARCH' named entity 'ECONOMIC' named entity 'ALTERING' named entity 'WORLDWIDE' named entity 'INTESTINAL' named entity 'EXTENSIVE' named entity 'CELLS'

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