About: Human iPSC-derived alveolar and airway epithelial cells can be cultured at air-liquid interface and express SARS-CoV-2 host factors

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About: Human iPSC-derived alveolar and airway epithelial cells can be cultured at air-liquid interface and express SARS-CoV-2 host factors Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Human iPSC-derived alveolar and airway epithelial cells can be cultured at air-liquid interface and express SARS-CoV-2 host factors
Creator	Ma, Liang Wilson, Andrew Kotton, Darrell Abo, Kristine Alysandratos, Konstantinos Beermann, Mary Hawkins, Finn Huang, Jessie Ikonomou, Laertis Lindstrom-Vautrin, Jonathan Matte, Taylor Mithal, Aditya Mostoslavsky, Gustavo Villacorta-Martin, Carlos Werder, Rhiannon
source	BioRxiv; Medline; PMC; WHO
abstract	Development of an anti-SARS-CoV-2 therapeutic is hindered by the lack of physiologically relevant model systems that can recapitulate host-viral interactions in human cell types, specifically the epithelium of the lung. Here, we compare induced pluripotent stem cell (iPSC)-derived alveolar and airway epithelial cells to primary lung epithelial cell controls, focusing on expression levels of genes relevant for COVID-19 disease modeling. iPSC-derived alveolar epithelial type II-like cells (iAT2s) and iPSC-derived airway epithelial lineages express key transcripts associated with lung identity in the majority of cells produced in culture. They express ACE2 and TMPRSS2, transcripts encoding essential host factors required for SARS-CoV-2 infection, in a minor subset of each cell sub-lineage, similar to frequencies observed in primary cells. In order to prepare human culture systems that are amenable to modeling viral infection of both the proximal and distal lung epithelium, we adapt iPSC-derived alveolar and airway epithelial cells to two-dimensional air-liquid interface cultures. These engineered human lung cell systems represent sharable, physiologically relevant platforms for SARS-CoV-2 infection modeling and may therefore expedite the development of an effective pharmacologic intervention for COVID-19.
has issue date	2020-06-04(xsd:dateTime)
bibo:doi	10.1101/2020.06.03.132639
bibo:pmid	32577635
has license	cc-by-nc-nd
sha1sum (hex)	87f5dc0842522c149c9057d70b77fe4c1113fc62
schema:url	https://doi.org/10.1101/2020.06.03.132639
resource representing a document's title	Human iPSC-derived alveolar and airway epithelial cells can be cultured at air-liquid interface and express SARS-CoV-2 host factors
has PubMed Central identifier	PMC7302183
has PubMed identifier	32577635
schema:publication	bioRxiv
resource representing a document's body	covid:87f5dc0842522c149c9057d70b77fe4c1113fc62#body_text
is schema:about of	named entity 'therapeutic' named entity 'majority' named entity 'subset' named entity 'distal' named entity 'human lung' named entity 'interface' named entity 'alveolar' named entity 'SARS-CoV-2' named entity 'HERE' named entity 'LEVELS' named entity 'ORDER' named entity 'LACK' named entity 'LINEAGE' named entity 'CELL' named entity 'OBSERVED' named entity 'FOCUSING' named entity 'LUNG' named entity 'They' named entity 'cell' named entity 'focusing' named entity 'epithelium' named entity 'epithelial' named entity 'genes' named entity 'interactions' named entity 'alveolar' named entity 'alveolar' named entity 'proximal and distal' named entity 'COVID-19 disease' named entity 'infection' named entity 'SARS-CoV-2' named entity 'infection' named entity 'induced pluripotent stem cell' named entity 'human lung' named entity 'epithelium' named entity 'lung' named entity 'cells' named entity 'SFTPA1' named entity 'epithelial' named entity 'Matrigel' named entity 'cell lines' named entity 'lung cancer' named entity 'Calu-3' named entity 'cell types' named entity 'logarithm' named entity 'ThermoFisher' named entity 'TMPRSS2' named entity 'SFTPD' named entity 'mucus' named entity 'Calu-3' named entity 'ACE2' named entity 'cell cycle' named entity 'Resource Sharing' named entity 'pathogenesis' named entity 'gastrointestinal' named entity 'Matsuyama' named entity 'ciliated' named entity 'ACE2' named entity 'epithelial cell' named entity 'Louvain method' named entity 'viral infection' named entity 'SFTPA2' named entity 'epithelial cells' named entity 'liquid interface' named entity 'ARDS' named entity 'digital gene expression' named entity 'NKX2-1' named entity 'dimensionality reduction'

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