About: Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples

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About: Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples
Creator	Ghildyal, Reena Yu, Xuelian
Source	Medline; PMC
abstract	Human rhinoviruses (HRVs) are positive-stranded RNA viruses belonging to the Enterovirus genus in the family of Picornaviridae. Identification of the specific strain in HRV disease has been difficult because the traditional serological method is insensitive, labor intensive, and cumbersome. With the fast progress in molecular biological technique, more sensitive and faster molecular methods have been developed, such as polymerase chain reaction (PCR), reverse transcriptase (RT)-PCR, and real-time RT-PCR. To improve the technique for defining the links between illnesses and specific strains of HRV, we developed RT-PCR specific for HRV as routine base. A multiplex RT-PCR that simultaneously identifies 12 respiratory viruses including HRV is also routinely used in our lab. Here we have described the specific steps of methods for identification of HRV from clinical samples, such as sample preparation, isolation of total RNA, nested-RT-PCR for HRV, Seeplex(®) RV15 ACE Detection method, gel electrophoresis, how to use the QIAxcel(®) capillary electrophoresis system, and results interpretation.
has issue date	2014-09-09(xsd:dateTime)
bibo:doi	10.1007/978-1-4939-1571-2_2
bibo:pmid	25261303
has license	no-cc
sha1sum (hex)	871e4ddd35c70372f5277d9da89a82895325883e
schema:url	https://doi.org/10.1007/978-1-4939-1571-2_2
resource representing a document's title	Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples
has PubMed Central identifier	PMC7120866
has PubMed identifier	25261303
schema:publication	Rhinoviruses
resource representing a document's body	covid:871e4ddd35c70372f5277d9da89a82895325883e#body_text
is schema:about of	named entity 'interpretation' named entity 'steps' named entity 'gel electrophoresis' named entity 'RT-PCR' named entity 'strains' named entity 'CLINICAL' named entity 'TECHNIQUE' named entity 'NESTED' named entity 'RT-PCR' named entity 'ISOLATION' named entity 'FASTER' named entity 'STRAIN' named entity 'INCLUDING' named entity 'GEL ELECTROPHORESIS' named entity 'ENTEROVIRUS' named entity 'PCR' named entity 'FAMILY' named entity 'RESULTS' named entity 'SIMULTANEOUSLY' named entity 'HUMAN' named entity 'INTERPRETATION' named entity 'RESPIRATORY VIRUSES' named entity 'BIOLOGICAL' named entity 'CULT' named entity 'BELONGING' named entity 'ELECTROPHORESIS SYSTEM' named entity 'ACE' named entity 'USE' named entity 'MULTIPLEX' named entity 'TRADITIONAL' named entity 'DISEASE' named entity 'SAMPLES' named entity 'FOR DIAGNOSIS' named entity 'MOLECULAR' named entity 'INSENSITIVE' named entity 'OUR' named entity 'RHINOVIRUS INFECTION' named entity 'SEROLOGICAL METHOD' named entity 'SENSITIVE' named entity 'BASE' named entity 'CATION' named entity 'CHAPTER' named entity 'NESTED' named entity 'PROGRESS' named entity 'TOTAL RNA' named entity 'HOW' named entity 'HAVE' named entity 'DESCRIBED' named entity 'HERE' named entity 'RHINOVIRUSES' named entity 'GENUS' named entity 'METHODS' named entity 'USED' named entity 'POSITIVE' named entity 'SAMPLES' named entity 'CAPILLARY ELECTROPHORESIS' named entity 'LINKS' named entity 'LAB' named entity 'REVERSE TRANSCRIPTASE' named entity 'DETECTION METHOD' named entity 'RT-PCR' named entity 'REAL-TIME RT-PCR' named entity 'ROUTINE' named entity 'STEPS' named entity 'FAST' named entity 'HRV' named entity 'MULTIPLEX' named entity 'CLINICAL' named entity 'LABOR' named entity 'ILLNESSES' named entity 'STRAINS' named entity 'POLYMERASE CHAIN REACTION' named entity 'SAMPLE PREPARATION' named entity 'RNA VIRUSES' named entity 'PICORNAVIRIDAE' named entity 'With' named entity 'belonging' named entity 'RT-PCR' named entity 'RNA' named entity 'Enterovirus'

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