About: Induction of T-cell response by a DNA vaccine encoding a novel HLA-A*0201 severe acute respiratory syndrome coronavirus epitope

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About: Induction of T-cell response by a DNA vaccine encoding a novel HLA-A*0201 severe acute respiratory syndrome coronavirus epitope Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Induction of T-cell response by a DNA vaccine encoding a novel HLA-A*0201 severe acute respiratory syndrome coronavirus epitope
Creator	Chan, Kin-Tak Cheng, Samuel Chak-Sum Cheung, Ying-Kit Xie, Yong Sin, Wan-Yee
source	Elsevier; Medline; PMC
abstract	Abstract The severe acute respiratory syndrome coronavirus nucleocapsid protein (SARS-CoV N) is one of the major targets for SARS vaccine due to its high potency in triggering immune responses. In this study, we have identified a novel HLA-A*0201 restricted epitope, N220 (LALLLLDRL), of the SARS-CoV N-protein through bioinformatics analysis. The N-protein peptide N220 shows a high binding affinity towards human MHC class I in T2-cells, and is capable of activating cytotoxic T-cells in human peripheral blood mononuclear cells (PBMCs). The application of using the N220 peptide sequence with a single-chain-trimer (SCT) approach to produce a potential DNA vaccine candidate was investigated in HLA-A2.1Kb transgenic mice. Cytotoxicity assay clearly showed that the T-cells obtained from the vaccinated animals were able to kill the N-protein expressing cells with a cytotoxicity level of 86% in an effector cells/target cells ratio of 81:1 one week after the last vaccination, which is significantly higher than other N-protein peptides previously described. The novel immunogenic N-protein peptide revealed in the present study provides valuable information for therapeutic SARS vaccine design.
has issue date	2007-08-10(xsd:dateTime)
bibo:doi	10.1016/j.vaccine.2007.05.025
bibo:pmid	17629360
has license	els-covid
sha1sum (hex)	866943e037d3b683d0405a9d65d94568074ed592
schema:url	https://doi.org/10.1016/j.vaccine.2007.05.025
resource representing a document's title	Induction of T-cell response by a DNA vaccine encoding a novel HLA-A*0201 severe acute respiratory syndrome coronavirus epitope
has PubMed Central identifier	PMC7115375
has PubMed identifier	17629360
schema:publication	Vaccine
resource representing a document's body	covid:866943e037d3b683d0405a9d65d94568074ed592#body_text
is schema:about of	named entity 'protein' named entity 'severe acute respiratory syndrome coronavirus' named entity 'restricted' covid:arg/866943e037d3b683d0405a9d65d94568074ed592 named entity 'peptide sequence' named entity 'vaccination' named entity 'revealed' named entity 'SARS-CoV' named entity 'cells' named entity 'peptide' named entity 'triggering' named entity 'high' named entity 'activating' named entity 'study' named entity 'HLA-A2' named entity 'cytotoxic T-cells' named entity 'vaccine' named entity 'SARS' named entity 'peripheral blood mononuclear cells' named entity 'protein' named entity 'T-cells' named entity 'vaccination' named entity 'nucleocapsid protein' named entity 'protein' named entity 'peptides' named entity 'DNA vaccine' named entity 'severe acute respiratory syndrome coronavirus' named entity 'DNA vaccine' named entity 'peptides' named entity 'bioinformatic analysis' named entity 'DNA vaccine' named entity 'covalently linked' named entity 'covalently linked' named entity 'DNA fragments' named entity 'Amersham' named entity 'peptide' named entity 'flu' named entity 'antibody' named entity 'peptides' named entity 'antigen processing' named entity 'dendritic cells' named entity 'target peptide' named entity 'HLA-A2' named entity 'peptide' named entity 'cloned' named entity 'SARS' named entity 'protein' named entity 'DNA sequence' named entity 'autologous' named entity 'National Institutes of Health' named entity 'cytotoxicity' named entity 'SARS' named entity 'epitope' named entity 'amino acid sequence' named entity 'protein' named entity 'cytotoxic T-cell' named entity 'high performance liquid chromatography' named entity 'gene' named entity 'protein' named entity 'Novagen' named entity 'tumor necrosis factor-alpha' named entity 'bioinformatic analysis' named entity 'antigen' named entity 'recombinant protein' named entity 'vaccine' named entity 'DNA sequence' named entity 'fibroblasts' named entity 'cDNA' named entity 'PBMCs' named entity 'Peptides' named entity 'chaperone' named entity 'potency' named entity 'HLA-A2' named entity 'proteins expressed' named entity 'antigen presenting cells' named entity 'dendritic cells' named entity 'peptide'

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