About: Molecular Detection of SARS-CoV-2 Infection in FFPE Samples and Histopathologic Findings in Fatal SARS-CoV-2 Cases

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About: Molecular Detection of SARS-CoV-2 Infection in FFPE Samples and Histopathologic Findings in Fatal SARS-CoV-2 Cases Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular Detection of SARS-CoV-2 Infection in FFPE Samples and Histopathologic Findings in Fatal SARS-CoV-2 Cases
Creator	Gilmore, Hannah Harding, Clifford Harper, Holly Koeth, Aaron Nezami, Behtash Sadri, Navid Sekulic, Miroslav Sekulic, Simona Shen, Daniel
Source	Medline; PMC
abstract	OBJECTIVES: To report methods and findings of 2 autopsies with molecular evaluation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positive individuals. METHODS: Postmortem examination was completed following Centers for Disease Control and Prevention public guidelines. Numerous formalin-fixed paraffin-embedded (FFPE) tissue types from each case were surveyed for SARS-CoV-2 RNA by quantitative reverse transcription polymerase chain reaction (qRT-PCR). SARS-CoV-2 viral genome was sequenced by next-generation sequencing (NGS) from FFPE lung tissue blocks. RESULTS: Postmortem examinations revealed diffuse alveolar damage, while no viral-associated hepatic, cardiac, or renal damage was observed. Viral RNA was detected in lungs, bronchi, lymph nodes, and spleen in both cases using qRT-PCR method. RNA sequencing using NGS in case 1 revealed mutations most consistent with Western European Clade A2a with ORF1a L3606F mutation. CONCLUSIONS: SARS-CoV-2 testing and viral sequencing can be performed from FFPE tissue. Detection and sequencing of SARS-CoV-2 in combination with morphological findings from postmortem tissue examination can aid in gaining a better understanding of the virus’s pathophysiologic effects on human health.
has issue date	2020-05-26(xsd:dateTime)
bibo:doi	10.1093/ajcp/aqaa091
bibo:pmid	32451533
has license	no-cc
sha1sum (hex)	864b7d230c62ec3c77777210fb3608c52f6b0197
schema:url	https://doi.org/10.1093/ajcp/aqaa091
resource representing a document's title	Molecular Detection of SARS-CoV-2 Infection in FFPE Samples and Histopathologic Findings in Fatal SARS-CoV-2 Cases
has PubMed Central identifier	PMC7314275
has PubMed identifier	32451533
schema:publication	Am J Clin Pathol
resource representing a document's body	covid:864b7d230c62ec3c77777210fb3608c52f6b0197#body_text
is schema:about of	named entity 'severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)' named entity 'positive' named entity 'Infection' named entity 'medical history' named entity 'hyaline' named entity 'nasal cannula' named entity 'histomorphologic' named entity 'pressor' named entity 'pancreas' named entity 'large cell carcinoma' named entity 'granulomata' named entity 'oxygen' named entity 'computed tomography' named entity 'myocardium' named entity 'Ion' named entity 'microthrombi' named entity 'bronchopneumonia' named entity 'primer' named entity 'medial aspect' named entity 'interstitial fibrosis' named entity 'SARS-CoV-2' named entity 'diffuse alveolar damage' named entity 'RNA' named entity 'genome' named entity 'parenchyma' named entity 'kidneys' named entity 'distal' named entity 'alveolar' named entity 'SARS-CoV-2' named entity 'liver enzymes' named entity 'anticoagulation' named entity 'renin' named entity 'hyaline' named entity 'genome' named entity 'afebrile' named entity 'cardiomegaly' named entity 'tachycardia' named entity 'kidneys' named entity 'bronchus' named entity 'main bronchi' named entity 'exudative' named entity 'angiotensin' named entity 'clade' named entity 'acute phase' named entity 'SARS-CoV-2' named entity 'left lower lobe' named entity 'congestive heart failure' named entity 'tissue samples' named entity 'carotid endarterectomy' named entity 'evolution' named entity 'tissue sections' named entity 'coronary arteries' named entity 'metastatic' named entity 'diabetes' named entity 'Coronavirus' named entity 'alveolar' named entity 'left-sided' named entity 'acute respiratory failure' named entity 'epithelial cells' named entity 'breath sounds' named entity 'aldosterone' named entity 'SARS-CoV' named entity 'malignancy' named entity 'mass lesion' named entity 'genomic sequencing' named entity 'postmortem' named entity 'bradycardic' named entity 'Thermo Fisher' named entity 'emphysematous' named entity 'upper airway' named entity 'mass lesion' named entity 'antiviral medication' named entity 'lungs'

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