About: Identification of aromatic amino acid residues in conserved region VI of the large polymerase of vesicular stomatitis virus is essential for both guanine-N-7 and ribose 2'-O methyltransferases

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About: Identification of aromatic amino acid residues in conserved region VI of the large polymerase of vesicular stomatitis virus is essential for both guanine-N-7 and ribose 2'-O methyltransferases Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of aromatic amino acid residues in conserved region VI of the large polymerase of vesicular stomatitis virus is essential for both guanine-N-7 and ribose 2'-O methyltransferases
Creator	Li, Jianrong Ma, Yuanmei Wei, Yongwei Zhang, Xiaodong Hu, Songhua
Source	Elsevier; Medline; PMC
abstract	Abstract Non-segmented negative-sense RNA viruses possess a unique mechanism for mRNA cap methylation. For vesicular stomatitis virus, conserved region VI in the large (L) polymerase protein catalyzes both guanine-N-7 (G-N-7) and ribose 2'-O (2'-O) methyltransferases, and the two methylases share a binding site for the methyl donor S-adenosyl-l-methionine. Unlike conventional mRNA cap methylation, the 2'-O methylation of VSV precedes subsequent G-N-7 methylation. In this study, we found that individual alanine substitutions in two conserved aromatic residues (Y1650 and F1691) in region VI of L protein abolished both G-N-7 and 2'-O methylation. However, replacement of one aromatic residue with another aromatic residue did not significantly affect the methyltransferase activities. Our studies provide genetic and biochemical evidence that conserved aromatic residues in region VI of L protein essential for both G-N-7 and 2'-O methylations. In combination with the structural prediction, our results suggest that these aromatic residues may participate in RNA recognition.
has issue date	2010-12-20(xsd:dateTime)
bibo:doi	10.1016/j.virol.2010.09.017
bibo:pmid	20961592
has license	els-covid
sha1sum (hex)	86334a1a36bd178b5a3b2c1f6943d2ac6fa40e27
schema:url	https://doi.org/10.1016/j.virol.2010.09.017
resource representing a document's title	Identification of aromatic amino acid residues in conserved region VI of the large polymerase of vesicular stomatitis virus is essential for both guanine-N-7 and ribose 2'-O methyltransferases
has PubMed Central identifier	PMC7111938
has PubMed identifier	20961592
schema:publication	Virology
resource representing a document's body	covid:86334a1a36bd178b5a3b2c1f6943d2ac6fa40e27#body_text
is schema:about of	named entity 'possess' named entity 'Virology' named entity 'conventional' named entity 'RNA' covid:arg/86334a1a36bd178b5a3b2c1f6943d2ac6fa40e27 named entity 'antigenomic' named entity 'RNA' named entity 'methylations' named entity 'alanine' named entity 'However' named entity 'residues' named entity 'region VI' named entity 'protein' named entity 'controls' named entity 'biochemical' named entity 'protein' named entity 'polymerase' named entity 'methylations' named entity 'polymerase' named entity 'RNA synthesis' named entity 'ribose' named entity 'methyl' named entity 'aromatic residues' named entity 'Non-segmented' named entity 'methylation' named entity 'aromatic amino acid' named entity 'SAM' named entity 'VSV' named entity 'amino acid' named entity 'ribose' named entity 'substrate' named entity 'Decroly' named entity 'methylation' named entity 'GTP' named entity 'Virus' named entity 'mutagenesis' named entity 'mRNA' named entity 'mutagenesis' named entity 'aromatic residues' named entity 'nucleocapsid' named entity 'amino acids' named entity 'mRNA' named entity 'transcription' named entity 'substrate' named entity 'adenyl' named entity 'nucleocapsid' named entity 'aromatic rings' named entity 'Bradford assay' named entity 'aromatic residues' named entity 'hydrolysis' named entity 'binding site' named entity 'nucleocapsid' named entity 'SAM' named entity 'Villarreal' named entity 'fetal bovine serum' named entity 'methyltransferase' named entity 'transcription' named entity 'VSV' named entity 'VSV' named entity 'SAM' named entity 'RNA viruses' named entity 'multiplicity of infection' named entity 'methylations' named entity 'centrifugation' named entity 'Recombinant' named entity 'RNA' named entity 'inoculum' named entity 'substrate' named entity 'methylation' named entity 'RNAs' named entity 'RNA' named entity 'nucleocapsid' named entity 'aromatic' named entity 'WNV' named entity 'methylation' named entity 'Aromatic residues' named entity 'SAM'

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