About: Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway

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About: Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway
Creator	Li, Yan Lu, Jing Chi, Ying Bian, Qian Guo, Hongxiong Zhang, L
Source	Medline; PMC
abstract	Outbreaks of avian influenza A (H5N1) virus infection have significant health and economic consequences. Non-structural protein 1 (NS1) is an essential virulence factor of the highly pathogenic H5N1 avian influenza virus and of the apoptosis associated with the pathogenesis of H5N1. Previous studies have revealed that the NS1 protein is able to induce apoptosis via an extrinsic pathway. However, it remains unclear whether the intrinsic pathway is also associated with this apoptosis. The present study used a clone of the NS1 gene from avian influenza A/Jiangsu/1/2007 and observed the localization of the NS1 protein and cytochrome c release from mitochondria and the change of mitochondrial membrane potential (MMP) in lung cancer cells. Cytotoxicity was detected using an MTT assay and the number of apoptotic cells was counted using a flow cytometer. Following the isolation of mitochondria, western blotting was performed to compare cytochrome c release from the mitochondria in cells before and after apoptosis. The change of MMP was detected using JC-1 staining. Furthermore, the results reveal that the majority of the NS1 protein was localized in the cell nucleus, and that it may induce apoptosis of human lung epithelial cells. The apoptosis occurred with marked cytochrome c release from mitochondria and a change of the MMP. This indicated that the NS1 protein may be associated with apoptosis induced by an intrinsic mitochondrial pathway.
has issue date	2017-08-28(xsd:dateTime)
bibo:doi	10.3892/etm.2017.5056
bibo:pmid	29067097
has license	cc-by-nc-nd
sha1sum (hex)	854545208a6bc7cafa8744604899e32237ff2047
schema:url	https://doi.org/10.3892/etm.2017.5056
resource representing a document's title	Highly pathogenic avian influenza A virus H5N1 non-structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway
has PubMed Central identifier	PMC5647739
has PubMed identifier	29067097
schema:publication	Exp Ther Med
resource representing a document's body	covid:854545208a6bc7cafa8744604899e32237ff2047#body_text
is schema:about of	named entity 'MITOCHONDRIAL MEMBRANE POTENTIAL' named entity 'H5N1' named entity 'LOCALIZED' named entity 'ESSENTIAL' named entity 'PROTEIN ' named entity 'EXTRINSIC PATHWAY' named entity 'INTRINSIC MITOCHONDRIAL PATHWAY' named entity 'PATHOGENESIS' named entity 'MARKED' named entity 'REVEALED' named entity 'RELEASE' named entity 'HAVE' named entity 'VIRULENCE FACTOR' named entity 'STUDIES' named entity 'PATHOGENIC' named entity 'USING' named entity 'TO COMPARE' named entity 'DETECTED' named entity 'ASSOCIATED WITH' named entity 'OBSERVED' named entity 'study' named entity 'mitochondria' named entity 'This' named entity 'avian influenza' named entity 'gene' named entity 'protein' named entity 'human lung' named entity 'APOPTOTIC' named entity 'INTRINSIC PATHWAY' named entity 'NON-STRUCTURAL PROTEIN 1' named entity 'AVIAN INFLUENZA A VIRUS' named entity 'AVIAN INFLUENZA A' named entity 'HUMAN LUNG' named entity 'CANCER CELLS' named entity 'PERFORMED' named entity 'INDICATED' named entity 'NS1' named entity 'ISOLATION' named entity 'APOPTOSIS' named entity 'CELL NUCLEUS' named entity 'H5N1' named entity 'APOPTOTIC' named entity 'GENE' named entity 'USED' named entity 'CYTOCHROME C' named entity 'MAY BE' named entity 'LUNG CANCER' named entity 'OCCURRED' named entity 'STUDY' named entity 'WESTERN BLOTTING' named entity 'MMP' named entity 'FLOW CYTOMETER' named entity 'CYTOTOXICITY' named entity '2F1' named entity 'THE CHANGE' named entity 'CELLS' named entity 'MTT ASSAY' named entity 'NUMBER OF' named entity 'FOLLOWING' named entity 'HIGHLY PATHOGENIC AVIAN INFLUENZA' named entity 'ASSOCIATED WITH' named entity 'PREVIOUS' named entity 'NS1 PROTEIN' named entity 'PRESENT' named entity 'AVIAN INFLUENZA VIRUS' named entity 'LOCALIZATION' named entity 'INDUCE' named entity 'EPITHELIAL CELLS' named entity 'INTRINSIC MITOCHONDRIAL PATHWAY' named entity 'ACTIVATION' named entity 'CHANGE' named entity 'INDUCED' named entity 'JC-1' named entity 'HIGHLY' named entity 'UNCLEAR' named entity 'CLONE'

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