About: Canine coronavirus induces apoptosis in cultured cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Canine coronavirus induces apoptosis in cultured cells
Creator	Buonavoglia, C Elia, G Bedini, B Di Trani, L Franco, M Gatto, I Ruggieri, A Vignolo, E
Source	Elsevier; Medline; PMC
abstract	Canine coronavirus (CCoV) is widespread in dogs in several countries and causes mild enteric illness evolving to severe enteritis in young pups. In in vitro cultures canine coronaviruses generally induce extensive cell death, however nature of the events leading to cell death remains largely unknown. We analysed the induction of cytopathic effect by CCoV in a canine fibrosarcoma cell line (A-72) in order to characterize the apoptotic effect in homologous cell system. Following CCoV infection A-72 cell line, which is permissive to CCoV, showed reduced growth rate, as detected by MTT assay, a standard colorimetric assay for measuring cellular proliferation, and underwent to apoptotic death. Starting from 24 h after CCoV infection, cells morphology appeared dramatically changed, with cells rounding and detachment from culture surface. Morphologic and biochemical features of apoptosis, such as blebbing of the plasma membrane, translocation of phosphatidilserine to cell surface and annexin V positive staining, nuclear fragmentation, apoptotic bodies formation and DNA laddering, were detected in CCoV-infected cells. Propidium iodide staining of infected culture indicated the appearance of hypodiploid DNA peak corresponding to apoptotic cell population. Commonly to other animal coronavirus infection caspase-3 is likely to contribute to the execution phase of apoptosis induced by CCoV in A-72 cells since we found activation of enzymatic activity as well as procaspase-3 activating cleavage. Apoptotic death of infected cells is detrimental as it causes cell and tissue destruction as well as inflammatory responses. Therefore in the case of CCoV associated gastroenteritis, apoptosis of epithelial mucosa cells may be responsible for pathology induced by CCoV infection.
has issue date	2007-03-31(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2006.12.016
bibo:pmid	17254720
has license	no-cc
sha1sum (hex)	844f3130ada716092ea078d26dc1bfed153ca025
schema:url	https://doi.org/10.1016/j.vetmic.2006.12.016
resource representing a document's title	Canine coronavirus induces apoptosis in cultured cells
has PubMed Central identifier	PMC7117493
has PubMed identifier	17254720
schema:publication	Vet Microbiol
resource representing a document's body	covid:844f3130ada716092ea078d26dc1bfed153ca025#body_text
is schema:about of	named entity 'CANINE CORONAVIRUS' named entity 'DOGS' named entity 'MILD' named entity 'ENTERITIS' named entity 'COUNTRIES' named entity 'CAUSES' named entity 'APOPTOSIS' named entity 'CULTURED CELLS' named entity 'PUPS' named entity 'EVOLVING' named entity 'WIDESPREAD' named entity 'SEVERE' named entity 'YOUNG' named entity 'ILLNESS' named entity 'ENTERIC' named entity 'CANINE CORONAVIRUS' named entity 'enteritis' named entity 'agarose gel' named entity 'cross-reactivity' named entity 'cDNA' named entity 'monoclonal antibody' named entity 'apoptosis' named entity 'caspase-3' named entity 'virus' named entity 'Hoechst 33342' named entity 'phase-contrast microscope' named entity 'vacuolization' named entity 'morphological' named entity 'parasites' named entity 'PBS' named entity 'biological activity' named entity 'TGEV' named entity 'caspase-3' named entity 'Triton X-100' named entity 'canine' named entity 'plasma membrane' named entity 'biochemical' named entity 'cell line' named entity 'cytoplasm' named entity 'HCoV-NL63' named entity 'cell death' named entity 'animal reservoir' named entity 'mAb' named entity 'inflammatory' named entity 'phosphatidylserine' named entity 'SARS-CoV' named entity 'proteinase' named entity 'cell line' named entity 'coronaviruses' named entity 'cell lysates' named entity 'mucosa' named entity 'staining' named entity 'mAbs' named entity 'biotin' named entity 'flow cytometer' named entity 'cellular proliferation' named entity 'annexin' named entity 'phylogenetic analysis' named entity 'SARS' named entity 'nm 48' named entity 'proenzyme' named entity 'apoptotic bodies' named entity 'physiological process' named entity 'Becton Dickinson' named entity 'apoptotic' named entity 'Hoechst 33342' named entity 'kDa' named entity 'porcine epidemic diarrhea virus' named entity 'caspase-3' named entity 'Proliferation rate' named entity 'Ponceau S' named entity 'apoptotic cells' named entity 'protein' named entity 'mAb'

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