About: Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency
Creator	Kim, Ho Shin, Eui-Cheol Chung, Jae-Hee Hong, Seon-Hui Kim, Tae-Shin Lee, Pedro Seo, Nari An, Hyun
Source	Elsevier; Medline; PMC
abstract	Abstract Interferon lambda 4 (IFNλ4) has been recently known and studied for its role in hepatitis C virus (HCV) infection, but its clinical potential is significantly hampered due to its poor expression in vitro. Our study reports the successful production of IFNλ4 from a mammalian cell line through a glycoengineering and structure-based approach. We introduced de novo N-glycosylation of IFNλ4, guided by structural analysis, and produced IFNλ4 variants in Expi293F that displayed improved expression and potency. To preserve the structure and functionality of IFNλ4, the model structure of the IFNλ4 signaling complex was analyzed and the N-glycosylation candidate sites were selected. The receptor binding activity of engineered IFNλ4 variants and their receptor-mediated signaling pathway were similar to the E. coli version of IFNλ4 (eIFNλ4), while the antiviral activity and induction levels of interferon-stimulated gene (ISG) were all more robust in our variants. Our engineered IFNλ4 variants may be further developed for clinical applications and utilized in basic research to decipher the immunological roles of IFNλ4.
has issue date	2020-01-31(xsd:dateTime)
bibo:doi	10.1016/j.cyto.2019.154833
bibo:pmid	31479875
has license	els-covid
sha1sum (hex)	83fb58d2d997738b64ed7855b23b066b9f9adf11
schema:url	https://doi.org/10.1016/j.cyto.2019.154833
resource representing a document's title	Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency
has PubMed Central identifier	PMC7129780
has PubMed identifier	31479875
schema:publication	Cytokine
resource representing a document's body	covid:83fb58d2d997738b64ed7855b23b066b9f9adf11#body_text
is schema:about of	named entity 'complex' named entity 'structure' named entity 'IN VITRO' named entity 'RECOMBINANT' named entity 'INFECTION' named entity 'BASED' named entity 'PRESERVE' named entity 'WILD-TYPE' named entity 'BUT' named entity 'STRUCTURAL ANALYSIS' named entity 'POOR' named entity 'MAMMALIAN' named entity 'DE NOVO' named entity 'PREVIOUSLY' named entity 'THEIR' named entity 'EXPRESSION' named entity 'TO PRODUCE' named entity 'DISPLAYED' named entity 'IMMUNOLOGICAL' named entity 'ANALYZED' named entity 'SELECTED' named entity 'DUE TO' named entity 'BINDING ACTIVITY' named entity 'MODEL' named entity 'INTRODUCED' named entity 'INTERFERON LAMBDA' named entity 'KNOWN' named entity 'ITS' named entity 'BB4' named entity 'VERSION' named entity 'POTENCY' named entity 'IFN' named entity 'ROLE' named entity 'RECENTLY' named entity 'SITES' named entity 'GENE' named entity 'CLINICAL' named entity 'ROLES' named entity 'BASIC RESEARCH' named entity 'INTERFERON' named entity 'POTENTIAL' named entity 'STUDY' named entity 'INDUCTION' named entity 'HEPATITIS C VIRUS' named entity 'APPLICATIONS' named entity 'TRANSIENT EXPRESSION' named entity 'POTENCY' named entity 'E. COLI' named entity 'IMPROVED' named entity 'SIGNIFICANT' named entity 'GLYCOENGINEERING' named entity 'ITS' named entity 'INTERFERON LAMBDA' named entity 'STRUCTURE' named entity 'PRODUCTIVITY' named entity 'VIRAL' named entity 'STIMULATED' named entity 'SUCCESSFUL' named entity 'FAILED' named entity 'GLYCOENGINEERING' named entity 'MAY BE' named entity 'FUNCTIONALITY' named entity 'RECEPTOR BINDING' named entity 'PRODUCTION' named entity 'REPORTS' named entity 'SIGNALING PATHWAY' named entity 'ENGINEERED' named entity 'APPROACH' named entity 'N-GLYCOSYLATION' named entity 'SIGNALING' named entity 'CANDIDATE' named entity 'DECIPHER' named entity 'VARIANTS' named entity 'AMOUNTS'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software