About: Cymbidium mosaic potexvirus isolate-dependent host movement systems reveal two movement control determinants and the coat protein is the dominant

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About: Cymbidium mosaic potexvirus isolate-dependent host movement systems reveal two movement control determinants and the coat protein is the dominant Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cymbidium mosaic potexvirus isolate-dependent host movement systems reveal two movement control determinants and the coat protein is the dominant
Creator	Chen, Cheng-En Lu, Hsiang-Chia Su, Hong-Ji Tsai, Meng-Hsiun Yeh, Hsin-Hung Wang, Hsiang-Iu
Source	Elsevier; Medline; PMC
abstract	Abstract Little is known about how plant viruses of a single species exhibit different movement behavior in different host species. Two Cymbidium mosaic potexvirus (CymMV) isolates, M1 and M2, were studied. Both can infect Phalaenopsis orchids, but only M1 can systemically infect Nicotiana benthamiana plants. Protoplast inoculation and whole-mount in situ hybridization revealed that both isolates can replicate in N. benthamiana; however, M2 was restricted to the initially infected cells. Genome shuffling between M1 and M2 revealed that two control modes are involved in CymMV host dependent movement. The M1 coat protein (CP) plays a dominant role in controlling CymMV movement between cells, because all chimeric CymMV viruses containing the M1 CP systemically infected N. benthamiana plants. Without the M1 CP, one chimeric virus containing the combination of the M1 triple gene block proteins (TGBps), the M2 5′ RNA (1–4333), and the M2 CP effectively moved in N. benthamiana plants. Further complementation analysis revealed that M1 TGBp1 and TGBp3 are co-required to complement the movement of the chimeric viruses in N. benthamiana. The amino acids within the CP, TGBp1 and TGBp3 which are required or important for CymMV M2 movement in N. benthamiana plants were mapped. The required amino acids within the CP map to the predicted RNA binding domain. RNA–protein binding assays revealed that M1 CP has higher RNA binding affinity than does M2 CP. Yeast two-hybrid assays to detect all possible interactions of M1 TGBps and CP, and only TGBp1 and CP self-interactions were observed.
has issue date	2009-05-25(xsd:dateTime)
bibo:doi	10.1016/j.virol.2009.02.049
bibo:pmid	19345971
has license	els-covid
sha1sum (hex)	82703ef405649117efd5d93cbceda6be903af4a0
schema:url	https://doi.org/10.1016/j.virol.2009.02.049
resource representing a document's title	Cymbidium mosaic potexvirus isolate-dependent host movement systems reveal two movement control determinants and the coat protein is the dominant
has PubMed Central identifier	PMC7103407
has PubMed identifier	19345971
schema:publication	Virology
resource representing a document's body	covid:82703ef405649117efd5d93cbceda6be903af4a0#body_text
is schema:about of	named entity 'MOVEMENT CONTROL' named entity 'orchids' named entity 'proteins' named entity 'RNA' named entity 'binding affinity' named entity 'cells' named entity 'host' named entity 'RNA BINDING' named entity 'VIRUSES' named entity 'COMPLEMENTATION' named entity 'INTERACTIONS' named entity 'REVEAL' named entity 'RNA BINDING DOMAIN' named entity 'BINDING AFFINITY' named entity 'RESTRICTED' named entity 'DEPENDENT' named entity 'DIFFERENT' named entity 'CONTROL' named entity 'COAT PROTEIN' named entity 'GENE' named entity 'ABOUT' named entity 'PROTEIN BINDING' named entity 'INVOLVED' named entity 'ORCHIDS' named entity 'MOUNT' named entity 'IN SITU HYBRIDIZATION' named entity 'MAPPED' named entity 'PROTOPLAST' covid:arg/82703ef405649117efd5d93cbceda6be903af4a0 named entity 'PROTEIN ' named entity 'MOVEMENT' named entity 'CYMBIDIUM MOSAIC POTEXVIRUS' named entity 'RNA' named entity 'SELF' named entity 'CONTROLLING' named entity 'SINGLE' named entity 'PLANT VIRUSES' named entity 'ANALYSIS' named entity 'DETERMINANTS' named entity 'HOST' named entity 'COAT PROTEIN' named entity 'DEPENDENT' named entity '80%' named entity 'CONTAINING' named entity 'PLANTS' named entity 'GENOME SHUFFLING' named entity 'PROTEIN ' named entity 'DOMINANT' named entity 'CELLS' named entity 'COMPLEMENT' named entity 'NICOTIANA BENTHAMIANA' named entity 'POSSIBLE' named entity 'YEAST TWO-HYBRID ASSAYS' named entity 'SPECIES' named entity 'OBSERVED' named entity 'EXHIBIT' named entity 'COMBINATION' named entity 'CHIMERIC' named entity 'AMINO ACIDS' named entity 'ISOLATE' named entity 'SYSTEMS' named entity 'HOW' named entity 'REPLICATE' named entity 'BUT' named entity 'HIGHER' named entity 'HOST' named entity 'INFECTED' named entity 'MOVED ' named entity 'LITTLE' named entity 'CYMBIDIUM MOSAIC POTEXVIRUS' named entity 'PHALAENOPSIS' named entity 'BLOCK' named entity 'DOES' named entity 'HOST SPECIES' named entity 'CHIMERIC VIRUS' named entity 'INFECT'

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