About: Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

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About: Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs
Creator	Zhang, Xiaoyan Zhang, Bo Yan, Bing Zhang, Chunyan Chen, Yan Gong, Jingsong Huang, Yongguo Li, Fanghe Liu, Jinghua
Source	PMC
abstract	Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), a hybridoma cell line strain secreting anti-HBs monoclonal antibody (mAb) (defined G6 mAb) was obtained. The cells grew and secreted mAb stably. Antibody titers in the culture supernatant and ascites were 2.048×10(6) and 4.096×10(6), respectively. By applying the anti-HBs G6 mAb and horseradish peroxidase (HRP)-labeled goat anti-HBs antibody, we developed a sandwich ELISA (defined G6m ELISA) for detecting both wild-type and immune escape mutant HBsAgs (IEM HBsAg). The assay was performed to detect 17 species of genome recombinant expression HBsAg, including two wild-type species and 15 IEM HBsAg species, which varied in the “a” determinant, in a group of patients infected with hepatitis B virus (HBV). The patients previously had a lower ELISA detection signal [(absorbance of patients/absorbance of normal people (P/N): 1.0–4.5)]. The results demonstrated that the sensitivity of this assay to wild-type HBsAg was no less than 0.125 μg/L; 12 of 15 IEM HBsAg species (P/N⩾2.5) were positive for G6 mAb. Of the positive IEM HBsAg species, two had a low absorbance value at 450 nm (A (450)), one had an intermediate A450 value and nine had a high A (450) value, which was 7.55%(mean), 59.4%and 92.1%–109.4% of the wild-type A (450) value, respectively. The two species with low A (450) value and the three negative species mutated at the bases 120–124 in the first loop of the HBV “a” determinant. Using the G6 ELISA and two commercial ELISA kits (A and B), 177 patients were tested. The G6 ELISA had a significantly higher detection rate than either commercial ELISAs (19.21% vs 14.89% and 6.21%, respectively; P<0.01, P<0.05, respectively).
has issue date	2009-07-04(xsd:dateTime)
bibo:doi	10.1007/s11684-009-0047-0
has license	no-cc
sha1sum (hex)	818a918df0ea080de66936a5cabca0bb396f5209
schema:url	https://doi.org/10.1007/s11684-009-0047-0
resource representing a document's title	Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs
has PubMed Central identifier	PMC7089361
schema:publication	Front Med China
resource representing a document's body	covid:818a918df0ea080de66936a5cabca0bb396f5209#body_text
is schema:about of	named entity 'WILD-TYPE' named entity 'CLINICAL APPLICATION' named entity 'patients' named entity 'IEM' named entity 'patients' named entity 'titers' named entity 'assay' named entity 'wild-type' named entity 'PREPARATION' named entity 'LESS THAN' named entity 'TESTED' named entity '120' named entity 'ABSORBANCE' named entity 'HIGH' named entity 'SANDWICH ELISA' named entity 'STRAIN' named entity 'MONOCLONAL ANTIBODY' named entity 'LOOP' named entity 'LOW' named entity 'PERFORMED' named entity '4.5' named entity 'INDIRECT' named entity '92.1' named entity 'MUTATED' named entity 'ANTI-HBS' named entity 'A GROUP' named entity 'ESCAPE MUTANT' named entity 'HBV' named entity 'SIGNAL' named entity 'EITHER' named entity 'CULTURE SUPERNATANT' named entity '0.05' named entity 'VALUE' named entity 'SECRETED' named entity 'DEMONSTRATED' named entity '0.01' named entity 'IMMUNE' named entity 'TO DETECT' named entity 'INFECTED' named entity '096' named entity 'STANDARD' named entity 'INCLUDING' named entity 'NEGATIVE' named entity 'POSITIVE' named entity 'LABELED' covid:arg/818a918df0ea080de66936a5cabca0bb396f5209 named entity 'ESCAPE MUTANT' named entity '124' named entity 'ANTIBODY TITERS' named entity 'WILD-TYPE' named entity '28A' named entity 'CELLS' named entity 'MONOCLONAL ANTIBODY' named entity 'ANTI-HBS' named entity 'TYPE A' named entity 'PEOPLE' named entity '048' named entity 'DETERMINANT' named entity 'HEPATITIS B VIRUS' named entity '28P' named entity 'HORSERADISH PEROXIDASE' named entity 'NORMAL' named entity '0.125' named entity 'A52' named entity 'APPLYING' named entity 'RECOMBINANT EXPRESSION' named entity 'GENOME' named entity 'USING' named entity 'SECRETING' named entity '22A' named entity 'RESULTS' named entity 'BASES' named entity '450' named entity 'ASCITES' named entity 'HBSAG'

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