About: Development of a Portable SPR Sensor for Nucleic Acid Detection

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a Portable SPR Sensor for Nucleic Acid Detection
Creator	Zhang, Hao Li, Yichen Chen, Yuanyuan Huang, Yafeng Liu, Luyao Qiu, Xianbo Yu, Duli Zhang, Lulu
Source	PMC
abstract	Nucleic acid detection is of great significance in clinical diagnosis, environmental monitoring and food safety. Compared with the traditional nucleic acid amplification detection method, surface plasmon resonance (SPR) sensing technology has the advantages of being label-free, having simple operation, and providing real-time detection. However, the angle scanning system in many SPR angle modulation detection applications usually requires a high-resolution stepper motor and complex mechanical structure to adjust the angle. In this paper, a portable multi-angle scanning SPR sensor was designed. The sensor only uses one stepping motor to rotate a belt, and the belt pulls the mechanical linkages of incident light and reflected light to move in opposite directions for achieving the SPR angle scanning mode that keeps the incident angle and reflected angle equal. The sensor has an angle scanning accuracy of 0.002°, response sensitivity of 3.72 × 10(−6) RIU (refractive index unit), and an angle scanning range of 30°–74°. The overall size of the system is only 480 mm × 150 mm × 180 mm. The portable SPR sensor was used to detect nucleic acid hybridization on a gold film chip modified with bovine serum albumin (BSA). The result revealed that the sensor had high sensitivity and fast response, and could successfully accomplish the hybridization detection of target DNA solution of 0.01 μmol/mL.
has issue date	2020-05-21(xsd:dateTime)
bibo:doi	10.3390/mi11050526
bibo:pmid	32455736
has license	cc-by
sha1sum (hex)	8118f91776766a853adc9ae7806ef57cbe751f87
schema:url	https://doi.org/10.3390/mi11050526
resource representing a document's title	Development of a Portable SPR Sensor for Nucleic Acid Detection
has PubMed Central identifier	PMC7281666
has PubMed identifier	32455736
schema:publication	Micromachines (Basel)
resource representing a document's body	covid:8118f91776766a853adc9ae7806ef57cbe751f87#body_text
is schema:about of	named entity 'stepper motor' named entity 'mechanical linkages' named entity 'incident angle' named entity 'directions' named entity 'sensor' named entity 'great' named entity 'µmol' named entity 'acid' named entity 'environmental monitoring' named entity 'Detection' named entity 'rotate' named entity 'operation' named entity 'sensitivity' named entity 'SPR' named entity 'sensor' named entity 'food safety' named entity 'angle' named entity 'detection' named entity 'SPR' named entity 'response' named entity 'micromachines' named entity 'µmol' named entity 'surface plasmon resonance' named entity 'refractive index' named entity 'SPR' named entity 'environmental monitoring' named entity 'nucleic acid hybridization' named entity 'stepper motor' named entity 'SPR' named entity 'hybridization' named entity 'SPR' named entity 'Sensor' named entity 'portable' named entity 'HCL' named entity 'DNA probes' named entity 'chromium' named entity 'NHS' named entity 'buffer solution' named entity 'reflected light' named entity 'refractive index' named entity 'prism' named entity 'Nucleic acid' named entity 'gas phase' named entity 'DNA probes' named entity 'angle modulation' named entity 'SPR' named entity 'µmol' named entity 'DNA probes' named entity 'complementary DNA' named entity 'prism' named entity 'reflected light' named entity 'molecule' named entity 'Vestri' named entity 'SPR' named entity 'refractive index' named entity 'μmol' named entity 'PH4' named entity 'linear correlation coefficient' named entity 'refractive index' named entity 'RIU' named entity 'sensor' named entity 'SPR' named entity 'image sensor' named entity 'glycerol' named entity 'glass slide' named entity 'sodium acetate' named entity 'SPR' named entity 'DNA probes' named entity 'refractive index' named entity 'SPR' named entity 'SPR' named entity 'oligomer' named entity 'Droplet' named entity 'deionized water'

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