About: The invasion of tobacco mosaic virus RNA induces endoplasmic reticulum stress-related autophagy in HeLa cells

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About: The invasion of tobacco mosaic virus RNA induces endoplasmic reticulum stress-related autophagy in HeLa cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The invasion of tobacco mosaic virus RNA induces endoplasmic reticulum stress-related autophagy in HeLa cells
Creator	Wang, Li Li, Yan Li, Jie Li, Li Huang, Wei Chen, Lang He, Yuling Liu, Changxuan Tan, Jinquan Tang, Zhiqing Xiao, Ruijing Yang, Ru Zheng, Xiaoling Zhu, Guoguo
Source	Medline; PMC
abstract	The ability of human cells to defend against viruses originating from distant species has long been ignored. Owing to the pressure of natural evolution and human exploration, some of these viruses may be able to invade human beings. If their ‘fresh’ host had no defences, the viruses could cause a serious pandemic, as seen with HIV, SARS (severe acute respiratory syndrome) and avian influenza virus that originated from chimpanzees, the common palm civet and birds, respectively. It is unknown whether the human immune system could tolerate invasion with a plant virus. To model such an alien virus invasion, we chose TMV (tobacco mosaic virus) and used human epithelial carcinoma cells (HeLa cells) as its ‘fresh’ host. We established a reliable system for transfecting TMV-RNA into HeLa cells and found that TMV-RNA triggered autophagy in HeLa cells as shown by the appearance of autophagic vacuoles, the conversion of LC3-I (light chain protein 3-I) to LC3-II, the up-regulated expression of Beclin1 and the accumulation of TMV protein on autophagosomal membranes. We observed suspected TMV virions in HeLa cells by TEM (transmission electron microscopy). Furthermore, we found that TMV-RNA was translated into CP (coat protein) in the ER (endoplasmic reticulum) and that TMV-positive RNA translocated from the cytoplasm to the nucleolus. Finally, we detected greatly increased expression of GRP78 (78 kDa glucose-regulated protein), a typical marker of ERS (ER stress) and found that the formation of autophagosomes was closely related to the expanded ER membrane. Taken together, our data indicate that HeLa cells used ERS and ERS-related autophagy to defend against TMV-RNA.
has issue date	2011-11-21(xsd:dateTime)
bibo:doi	10.1042/bsr20110069
bibo:pmid	21729006
has license	cc-by-nc
sha1sum (hex)	80a789fb3ed3279911f241585e315718b54c9a91
schema:url	https://doi.org/10.1042/bsr20110069
resource representing a document's title	The invasion of tobacco mosaic virus RNA induces endoplasmic reticulum stress-related autophagy in HeLa cells
has PubMed Central identifier	PMC3225954
has PubMed identifier	21729006
schema:publication	Biosci Rep
resource representing a document's body	covid:80a789fb3ed3279911f241585e315718b54c9a91#body_text
is schema:about of	named entity 'HELA CELLS' named entity 'HeLa cells' named entity 'AUTOPHAGY' named entity 'TOBACCO MOSAIC VIRUS' named entity 'ENDOPLASMIC RETICULUM STRESS' named entity 'RELATED' named entity 'VIRUS RNA' named entity 'INVASION' named entity 'RNA' named entity 'autophagy' named entity 'HeLa' named entity 'mouse hepatitis virus' named entity 'RNA' named entity 'autophagosomes' named entity 'CTAB' named entity 'autophagosomes' named entity 'transfection' named entity 'tobacco' named entity 'autophagy' named entity 'ssRNA' named entity 'ATF6' named entity 'viral replication' named entity 'replicase' named entity 'HeLa' named entity 'GFP' named entity 'secondary antibody' named entity 'cytoplasm' named entity 'virions' named entity 'Beclin1' named entity 'Lipofectamine' named entity 'ER membrane' named entity 'gene' named entity 'DMEM' named entity 'virus' named entity 'vacuoles' named entity 'GenBank' named entity 'autophagosomes' named entity 'vacuole' named entity 'vacuoles' named entity 'vacuole' named entity 'Lipofectamine' named entity 'RNAse' named entity 'cell death' named entity 'autophagy' named entity 'antiviral' named entity 'antibody' named entity 'HeLa' named entity 'TLR' named entity 'transfection' named entity 'electroporation' named entity 'Invitrogen' named entity 'transfected' named entity 'nucleic acid' named entity 'RNA' named entity 'Beclin1' named entity 'autophagy' named entity 'endocytosis' named entity 'viruses' named entity 'RNA' named entity 'RNA' named entity 'GRP78' named entity 'Beclin1' named entity 'cytoplasm' named entity 'viruses' named entity 'post-translationally' named entity 'vertebrate' named entity 'statistically significant' named entity 'cacodylate'

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