About: Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection

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About: Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection
Creator	Xu, Dong Cooper, Bret Joshi, Trupti Scheffler, Brian Stacey, Gary Thibivilliers, Sandra Campbell, Kimberly Nguyen, Henry
Source	Medline; PMC
abstract	BACKGROUND: Phaseolus vulgaris (common bean) is the second most important legume crop in the world after soybean. Consequently, yield losses due to fungal infection, like Uromyces appendiculatus (bean rust), have strong consequences. Several resistant genes were identified that confer resistance to bean rust infection. However, the downstream genes and mechanisms involved in bean resistance to infection are poorly characterized. RESULTS: A subtractive bean cDNA library composed of 10,581 unisequences was constructed and enriched in sequences regulated by either bean rust race 41, a virulent strain, or race 49, an avirulent strain on cultivar Early Gallatin carrying the resistance gene Ur-4. The construction of this library allowed the identification of 6,202 new bean ESTs, significantly adding to the available sequences for this plant. Regulation of selected bean genes in response to bean rust infection was confirmed by qRT-PCR. Plant gene expression was similar for both race 41 and 49 during the first 48 hours of the infection process but varied significantly at the later time points (72–96 hours after inoculation) mainly due to the presence of the Avr4 gene in the race 49 leading to a hypersensitive response in the bean plants. A biphasic pattern of gene expression was observed for several genes regulated in response to fungal infection. CONCLUSION: The enrichment of the public database with over 6,000 bean ESTs significantly adds to the genomic resources available for this important crop plant. The analysis of these genes in response to bean rust infection provides a foundation for further studies of the mechanism of fungal disease resistance. The expression pattern of 90 bean genes upon rust infection shares several features with other legumes infected by biotrophic fungi. This finding suggests that the P. vulgaris-U. appendiculatus pathosystem could serve as a model to explore legume-rust interaction.
has issue date	2009-04-27(xsd:dateTime)
bibo:doi	10.1186/1471-2229-9-46
bibo:pmid	19397807
has license	cc-by
sha1sum (hex)	8013bbf01944aa3f25d0faf16386ea51c7586066
schema:url	https://doi.org/10.1186/1471-2229-9-46
resource representing a document's title	Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection
has PubMed Central identifier	PMC2684537
has PubMed identifier	19397807
schema:publication	BMC Plant Biol
resource representing a document's body	covid:8013bbf01944aa3f25d0faf16386ea51c7586066#body_text
is schema:about of	named entity 'legume' covid:arg/8013bbf01944aa3f25d0faf16386ea51c7586066 named entity 'Phaseolus vulgaris' named entity 'common bean' named entity 'Common bean' named entity 'Data analysis' named entity 'Transcriptional' named entity 'introns' named entity 'gene expression' named entity 'soybean' named entity 'qRT-PCR' named entity 'NCBI' named entity 'peptidase' named entity 'soybean' named entity 'p-value' named entity 'germinating' named entity 'Genbank' named entity 'cDNA' named entity 'EST' named entity 'haustorium' named entity 'virulent' named entity 'vitamin B1' named entity 'Uniprot' named entity 'NCBI' named entity 'inoculation' named entity 'infection' named entity 'pycnia' named entity 'PCR' named entity 'host defenses' named entity 'fungus' named entity 'Uromyces' named entity '6-phosphogluconate dehydrogenase' named entity 'fungal infection' named entity 'biomass' named entity 'gene regulation' named entity 'reference genes' named entity 'contig' named entity 'infection' named entity 'legume crop' named entity 'expressed genes' named entity 'qRT-PCR' named entity 'hybridization' named entity 'cysteine-rich secretory protein' named entity 'epidermis' named entity 'pathogens' named entity 'ESTs' named entity 'ESTs' named entity 'infection' named entity 'Uromyces' named entity 'common bean' named entity 'p-value' named entity 'P. vulgaris' named entity 'nucleotides' named entity 'Colletotrichum lindemuthianum' named entity 'resistance (R) genes' named entity 'inoculation' named entity 'Uromyces' named entity 'bacterial blight' named entity 'gene expression' named entity 'PAMP' named entity 'EST' named entity 'SSH' named entity 'histidine kinase' named entity 'oomycete' named entity 'haustorium' named entity 'infection' named entity 'rust' named entity 'Applied Biosystems' named entity 'greenhouse' named entity 'protein' named entity 'ESTs' named entity 'surface of the leaf' named entity 'invertase' named entity 'cDNAs'

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