About: Use of the human colorectal adenocarcinoma (Caco‐2) cell line for isolating respiratory viruses from nasopharyngeal aspirates

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About: Use of the human colorectal adenocarcinoma (Caco‐2) cell line for isolating respiratory viruses from nasopharyngeal aspirates Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Use of the human colorectal adenocarcinoma (Caco‐2) cell line for isolating respiratory viruses from nasopharyngeal aspirates
Creator	Cheng, V Yuen, K Chan, J Chan, K Lau, S Li, W To, K Woo, P Tse, H Yan, M
Source	Medline; PMC
abstract	The human colorectal adenocarcinoma‐derived Caco‐2 cell line was evaluated as a means isolating common respiratory viruses from nasopharyngeal aspirates for the diagnosis of respiratory diseases. One hundred eighty‐nine direct immunofluorescence positive nasopharyngeal aspirates obtained from patients with various viral respiratory diseases were cultured in the presence of Caco‐2 cells or the following conventional cell lines: LLC‐MK2, MDCK, HEp‐2, and A549. Caco‐2 cell cultures effectively propagated the majority (84%) of the viruses present in nasopharyngeal aspirate samples compared with any positive cultures obtained using the panel cells (78%) or individual cell line MDCK (38%), HEp‐2 (21%), LLC‐MK2 (27%), or A549 (37%) cell lines. The differences against individual cell line were statistically significant (P = < 0.000001). Culture in Caco‐2 cells resulted in the isolation of 85% (36/42) of viruses which were not cultivated in conventional cell lines. By contrast, 80% (24/30) of viruses not cultivated in Caco‐2 cells were isolated using the conventional panel. The findings indicated that Caco‐2 cells were sensitive to a wide range of viruses and can be used to culture a broad range of respiratory viruses. J. Med. Virol. 85:874–879, 2013. © 2013 Wiley Periodicals, Inc.
has issue date	2013-03-18(xsd:dateTime)
bibo:doi	10.1002/jmv.23538
bibo:pmid	23508913
has license	no-cc
sha1sum (hex)	7e33ef858fd7b77e0bdb609dc740656cb4af193d
schema:url	https://doi.org/10.1002/jmv.23538
resource representing a document's title	Use of the human colorectal adenocarcinoma (Caco‐2) cell line for isolating respiratory viruses from nasopharyngeal aspirates
has PubMed Central identifier	PMC7167083
has PubMed identifier	23508913
schema:publication	J Med Virol
resource representing a document's body	covid:7e33ef858fd7b77e0bdb609dc740656cb4af193d#body_text
is schema:about of	named entity 'Caco-2' named entity 'cultures' named entity 'effectively' named entity 'Cell' named entity 'Line' named entity 'A549' named entity 'MAJORITY' named entity 'CELL LINES' named entity 'INDICATED' named entity 'NASOPHARYNGEAL' named entity 'CONVENTIONAL' named entity 'ISOLATION' named entity 'FINDINGS' named entity 'VARIOUS' named entity 'VIRAL' named entity 'positive' named entity 'viruses' named entity 'range' named entity 'Caco-2' named entity 'Caco-2' named entity 'diagnosis' named entity 'findings' named entity 'wide range' named entity 'The' named entity 'viral' named entity 'cell lines' named entity 'nasopharyngeal' named entity 'respiratory diseases' named entity 'MDCK' named entity 'nasopharyngeal aspirate' named entity 'A549' named entity 'statistically significant' named entity 'direct immunofluorescence' named entity 'Cell Line' named entity 'Viruses' named entity 'isolated' named entity 'individual' named entity 'direct immunofluorescence' named entity 'virus' named entity 'Caco-2' named entity 'laryngeal cancer' named entity 'influenza viruses' named entity 'viruses' named entity 'culture methods' named entity 'Caco-2' named entity 'Direct immunofluorescence' named entity 'A549' named entity 'carcinoma' named entity 'RSV' named entity 'Caco-2' named entity 'nasopharyngeal' named entity 'HEp-2' named entity 'RT-PCR' named entity 'Belgium' named entity 'fluorescence microscope' named entity 'Caco-2' named entity 'direct immunofluorescence' named entity 'antibiotics' named entity 'viruses' named entity 'Caco-2' named entity 'respiratory diseases' named entity 'cell lines' named entity 'enteric viruses' named entity 'A549' named entity 'RSV' named entity 'P-value' named entity 'Caco-2' named entity 'Jahangir' named entity 'antigen' named entity 'Table I' named entity 'A549' named entity 'viruses'

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