About: An acceptable method to evaluate the analytical performance of real-time fluorescent RT-PCR targeting SARS-CoV-2

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About: An acceptable method to evaluate the analytical performance of real-time fluorescent RT-PCR targeting SARS-CoV-2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	An acceptable method to evaluate the analytical performance of real-time fluorescent RT-PCR targeting SARS-CoV-2
Creator	Huang, Qing Chen, Min Huang, Wang, Liu Wei, Kun Huang, Qian Deng, Shao-Li Huang, Heng-Liu Lu, Wei-Ping Ren, Xiao-Dong Su, Ning Sun, Xian-Ge Tang, Qi-Mei
source	MedRxiv
abstract	It was necessary to carry out methodologies evaluations of real-time fluorescent reverse-transcription PCR (RT-PCR) targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Considering biosafety issues and lack of positive specimens in some special locations in China, the routine specimens from healthy individuals were used to perform methodologies evaluations, in which the indexes were the differences of quantification cycle values ({Delta}Cq) between human derived internal reference control (IRC) genes of a specimen and quality control (QC). Serial experiments were carried out to evaluate various factors that might affect aforementioned methodologies, such as types of virus transport mediums, methods of specimen pretreatment and template preparation, specimen vortex strength, specimen storage temperature and duration. The results showed that using {Delta}Cq values as indexes, among various factors that might affect analytical performance, it was better to store specimens in the normal saline transport mediums, inactivate pathogens using water or metal bath, release more virus particles from swabs by vortex mixing, extract nucleic acids with centrifuge methods, and perform amplification assays timely. Aforementioned opinions and optimum conditions were further confirmed by SRAS-CoV-2 pseudovirus and clinical positive specimens. Altogether, the results of this study indicated that the routine specimens from healthy individuals could be used to evaluate the analytical performance of real-time fluorescent RT-PCR targeting SRAS-CoV-2, of which the indexes were the {Delta}Cq values between IRC genes of a specimen and QC. This acceptable method was extremely valuable in both theoretical and practical significance under current pandemic of coronavirus disease 2019 (COVID-19).
has issue date	2020-05-21(xsd:dateTime)
bibo:doi	10.1101/2020.05.18.20105247
has license	medrxiv
sha1sum (hex)	7e32c882bd0eee6429e80027ee44b069d4229eaf
schema:url	https://doi.org/10.1101/2020.05.18.20105247
resource representing a document's title	An acceptable method to evaluate the analytical performance of real-time fluorescent RT-PCR targeting SARS-CoV-2
resource representing a document's body	covid:7e32c882bd0eee6429e80027ee44b069d4229eaf#body_text
is schema:about of	named entity 'derived' named entity 'factors' named entity 'amplification' named entity 'mediums' named entity 'genes' named entity 'particles' named entity 'individuals' named entity 'specimens' named entity 'SARS-CoV-2' named entity 'fluorescent' named entity 'performance' named entity 'NECESSARY' named entity 'ISSUES' named entity 'POSITIVE' named entity 'SPECIMEN STORAGE' named entity 'TEMPLATE' named entity 'THEORETICAL' named entity 'COULD BE' named entity 'USED' named entity 'ANALYTICAL' named entity 'REAL-TIME' named entity 'PERFORMANCE' named entity 'TARGETING' named entity 'DERIVED' named entity 'TIMELY' named entity 'INTERNAL' named entity 'evaluate' named entity 'temperature' named entity 'targeting' named entity 'bath' named entity 'acceptable' named entity 'IRC' named entity 'carry' named entity 'evaluations' named entity 'specimen' named entity 'specimens' named entity 'quantification' named entity 'optimum' named entity 'experiments' named entity 'analytical' named entity 'reverse-transcription' named entity 'quality control' named entity 'pseudovirus' named entity 'quantification cycle' named entity 'virus' named entity 'China' named entity 'SARS-CoV-2' named entity 'methodologies' named entity 'Roche' named entity 'oropharyngeal' named entity 'normal saline' named entity 'transport medium' named entity 'RT-PCR' named entity 'medRxiv' named entity 'China' named entity 'urine' named entity 'COVID-19' named entity 'China' named entity 'COVID' named entity 'RT-PCR' named entity 'COVID' named entity 'fluorescent' named entity 'preprint' named entity 'preprint' named entity 'nucleic acids' named entity 'SARS-CoV-2' named entity 'pseudovirus' named entity 'RT-PCR' named entity 'medRxiv' named entity 'SARS-CoV-2' named entity '0.01' named entity 'China' named entity 'oropharyngeal'

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