About: Detection of IgM responses to bovine respiratory syncytial virus by indirect ELISA following experimental infection and reinfection of calves: abolition of false positive and false negative results by pre-treatment of sera with protein-G agarose

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About: Detection of IgM responses to bovine respiratory syncytial virus by indirect ELISA following experimental infection and reinfection of calves: abolition of false positive and false negative results by pre-treatment of sera with protein-G agarose Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of IgM responses to bovine respiratory syncytial virus by indirect ELISA following experimental infection and reinfection of calves: abolition of false positive and false negative results by pre-treatment of sera with protein-G agarose
Creator	Elvander, M Graham, D Adair, B Foster, J Mawhinney, K Merza, M
Source	Elsevier; Medline; PMC
abstract	The IgM responses in three panels of sera generated by infection and reinfection of calves with bovine respiratory syncytial virus (BRSV) were measured by indirect ELISA (I-ELISA). The effect of depleting serum IgG by pre-treatment with protein G agarose (PGA) was evaluated. Following primary infection a weak IgM response was detected in the untreated sera of 3 out of 4 calves with maternally derived antibody (MDA). Both the magnitude and duration of the specific IgM responses in these calves were increased by pre-treatment with PGA. In addition, the fourth infected calf tested gave a single positive IgM result following PGA treatment. Transient or persistent IgM responses which were abolished by pre-treatment of sera with PGA were detected in 4/8 calves following reinfection. These were considered to be false positive results, consistent with the influence of IgM rheumatoid factor (IgM-RF). One of these calves and two additional calves showed transient increases in IgM which were resistant to PGA treatment. These were considered to represent specific IgM responses to reinfection. The results indicate the ability of PGA treatment to eliminate both false positive and false negative results and emphasise the necessity for controlling the influence of IgM-RF in IgM-specific indirect ELISAs.
has issue date	1999-10-01(xsd:dateTime)
bibo:doi	10.1016/s0165-2427(99)00086-0
bibo:pmid	10522785
has license	no-cc
sha1sum (hex)	7dd49f77c26add2a5aef6d2b4235eacee69b4f84
schema:url	https://doi.org/10.1016/s0165-2427%2899%2900086-0
resource representing a document's title	Detection of IgM responses to bovine respiratory syncytial virus by indirect ELISA following experimental infection and reinfection of calves: abolition of false positive and false negative results by pre-treatment of sera with protein-G agarose
has PubMed Central identifier	PMC7119899
has PubMed identifier	10522785
schema:publication	Vet Immunol Immunopathol
resource representing a document's body	covid:7dd49f77c26add2a5aef6d2b4235eacee69b4f84#body_text
is schema:about of	named entity 'INDIRECT' named entity 'AUTHOR' named entity 'IGM' named entity 'MATTER' named entity 'SPECIFIC' named entity 'Corresponding' named entity 'respiratory syncytial virus' named entity 'calves' named entity 'IGM' named entity 'FOLLOWING' named entity 'FALSE NEGATIVE' named entity 'CALVES' named entity 'INDIRECT' named entity 'RIGHTS' named entity 'GRAHAM' named entity 'NEGATIVE RESULTS' named entity 'EXPERIMENTAL INFECTION' named entity 'ELISA' named entity 'SERA' named entity 'AGAROSE' named entity 'BOVINE RESPIRATORY SYNCYTIAL VIRUS' named entity 'DETECTION' named entity 'REINFECTION OF' named entity 'SCIENCE' named entity 'PROTEIN' named entity 'RESPONSES' named entity 'FALSE POSITIVE' named entity 'INFLUENCE' named entity 'ELISAS' named entity 'PII' named entity '2427' named entity 'Detection' named entity 'IgM' named entity 'B.V' named entity 'protein' named entity 'bovine' named entity 'infection' named entity 'false positive and false negative' named entity 'IgM' named entity 'ELISAs' named entity 'IgM' named entity 'Elsevier Science' named entity 'respiratory syncytial virus' named entity 'front matter' named entity 'Elsevier Science' named entity 'IgM' named entity 'influence' named entity 'experimental' named entity 'infection' named entity 'sera' named entity 'Graham' named entity 'PII' named entity '1999' named entity 'All rights reserved' named entity 'responses' named entity 'false negative' named entity 'false positive' named entity 'author' named entity 'PRE-TREATMENT'
is part of of	The IgM responses in three panels of sera generated by infection and reinfection of calves with bovine respiratory syncytial virus (BRSV) were measured by indirect ELISA (I-ELISA). The effect of depleting serum IgG by pre-treatment with protein G agarose (PGA) was evaluated. Following primary infection a weak IgM response was detected in the untreated sera of 3 out of 4 calves with maternally derived antibody (MDA). Both the magnitude and duration of the specific IgM responses in these calves were increased by pre-treatment with PGA. In addition, the fourth infected calf tested gave a single positive IgM result following PGA treatment. Transient or persistent IgM responses which were abolished by pre-treatment of sera with PGA were detected in 4/8 calves following reinfection. These were considered to be false positive results, consistent with the influence of IgM rheumatoid factor (IgM-RF). One of these calves and two additional calves showed transient increases in IgM which were resistant to PGA treatment. These were considered to represent specific IgM responses to reinfection. The results indicate the ability of PGA treatment to eliminate both false positive and false negative results and emphasise the necessity for controlling the influence of IgM-RF in IgM-specific indirect ELISAs. Detection of IgM responses to bovine respiratory syncytial virus by indirect ELISA following experimental infection and reinfection of calves: abolition of false positive and false negative results by pre-treatment of sera with protein-G agarose covid:7dd49f77c26add2a5aef6d2b4235eacee69b4f84#body_text

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