About: Development and Evaluation of a SYBR Green-Based Real Time RT-PCR Assay for Detection of the Emerging Avian Influenza A (H7N9) Virus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development and Evaluation of a SYBR Green-Based Real Time RT-PCR Assay for Detection of the Emerging Avian Influenza A (H7N9) Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development and Evaluation of a SYBR Green-Based Real Time RT-PCR Assay for Detection of the Emerging Avian Influenza A (H7N9) Virus
Creator	Wang, Hua Qi, Xian Zhu, Zheng Zhou, Minghao Cui, Lunbiao Qi, Yuhua Shi, Zhiyang Fan, Huan
source	Medline; PMC
abstract	Most recently a novel avian-origin influenza A (H7N9) virus emerged in China and has been associated with lots of human infection and fatal cases. Genetic analysis of the viral genome revealed that this reassortant virus might be better adapted to humans than other avian influenza viruses. Molecular diagnostic methods are thus urgently needed in public health laboratories. In this study, a SYBR green-based one-step real time reverse transcription-PCR (RT-PCR) was developed to detect the novel H7N9 virus. The primer pairs on the basis of the hemagglutinin and neuraminidase gene sequences of H7N9 viruses amplified subtype-specific fragments with Tm values of 80.77±0.06°C for H7 and 81.20±0.17°C for N9 respectively. The standard curves showed a dynamic linear range across 6 log units of RNA copy number (10(6) to 10(1) copies/ µl) with a detection limit of 10 copies per reaction for both H7 and N9 assays by using serial ten-fold diluted in-vitro transcribed viral RNA. In addition, no cross-reactivity was observed with seasonal H1N1, H1N1 pdm09, H3N2, H5N1 and H9N2 viruses as well as other human respiratory viruses. When the assay was further evaluated in H7N9 virus infected clinical samples, positive amplification signals were obtained in all of the specimens with the accordance between H7 and N9 assays. Therefore, the established SYBR green-based real time RT-PCR assay could provide a rapid, sensitive, specific and reliable alternative approach with lower costs for high throughput screening of suspected samples from humans, animals and environments in first line public health laboratories.
has issue date	2013-11-20(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0080028
bibo:pmid	24278234
has license	cc-by
sha1sum (hex)	7d38e11db887bf824a89621f65d384ef45e14955
schema:url	https://doi.org/10.1371/journal.pone.0080028
resource representing a document's title	Development and Evaluation of a SYBR Green-Based Real Time RT-PCR Assay for Detection of the Emerging Avian Influenza A (H7N9) Virus
has PubMed Central identifier	PMC3835827
has PubMed identifier	24278234
schema:publication	PLoS One
resource representing a document's body	covid:7d38e11db887bf824a89621f65d384ef45e14955#body_text
is schema:about of	named entity 'H9N2' named entity 'PLoS ONE' named entity 'diagnostic' named entity 'positive' named entity 'SYBR' named entity 'rapid' named entity 'specific' named entity 'Evaluation' named entity 'BASED' named entity 'REAL TIME RT-PCR' named entity 'RESPIRATORY VIRUSES' named entity 'FATAL' named entity 'FOLD' named entity '2060' named entity 'PUBLIC HEALTH' named entity 'SIGNALS' named entity 'DYNAMIC' named entity 'ADAPTED' named entity 'DETECTION' named entity 'EVALUATED' named entity 'RT-PCR ASSAY' named entity 'PCR' named entity 'GENETIC ANALYSIS' named entity 'DEVELOPMENT' named entity 'OBSERVED' named entity 'LINE' named entity 'REACTION' named entity 'REAL TIME RT-PCR' named entity 'DETECTION LIMIT' named entity 'POSITIVE' named entity 'EVALUATION' named entity 'HEMAGGLUTININ' named entity 'REAL TIME' named entity 'INFLUENZA A' named entity 'SYBR GREEN' named entity 'PRIMER' named entity 'AMPLIFIED' named entity 'REVEALED' named entity 'CITATION' named entity 'H1N1' named entity 'COPY NUMBER' named entity 'PROVIDE' named entity 'REACTIVITY' named entity 'STUDY' named entity 'RELIABLE' named entity 'FRAGMENTS' named entity 'BETTER' named entity 'ACROSS' named entity 'LOWER' named entity 'AVIAN INFLUENZA A' named entity 'GENE' named entity 'ASSOCIATED WITH' named entity 'VIRUSES' named entity 'SUBTYPE' named entity 'ORG' named entity 'SERIAL' named entity 'DEVELOPMENT' named entity 'APPROACH' named entity 'H7N9' named entity 'ENVIRONMENTS' named entity 'SEASONAL' named entity 'SUSPECTED' named entity 'INFLUENZA VIRUSES' named entity 'NOVEL' named entity 'VALUES' named entity 'WWW' named entity 'SPECIMENS' named entity 'TRANSCRIBED' named entity 'H3N2' named entity 'LOG' named entity 'CASES' named entity 'REVERSE TRANSCRIPTION' named entity 'AVIAN' named entity 'UNITS'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software