About: Temperature Sensitivity: A Potential Method for the Generation of Vaccines against the Avian Coronavirus Infectious Bronchitis Virus

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About: Temperature Sensitivity: A Potential Method for the Generation of Vaccines against the Avian Coronavirus Infectious Bronchitis Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Temperature Sensitivity: A Potential Method for the Generation of Vaccines against the Avian Coronavirus Infectious Bronchitis Virus
Creator	Britton, Paul Keep, Sarah Bickerton, Erica Stevenson-Leggett, Phoebe Vervelde, Lonneke Maier, Helena Oade, Michael Stuart, Jamie Steyn, Angela Webb, Isobel
Source	Medline; PMC
abstract	The Gammacoronavirus infectious bronchitis virus (IBV) is a highly contagious economically important respiratory pathogen of domestic fowl. Reverse genetics allows for the molecular study of pathogenic determinants to enable rational vaccine design. The recombinant IBV (rIBV) Beau-R, a molecular clone of the apathogenic Beaudette strain, has previously been investigated as a vaccine platform. To determine tissues in which Beau-R could effectively deliver antigenic genes, an in vivo study in chickens, the natural host, was used to compare the pattern of viral dissemination of Beau-R to the pathogenic strain M41-CK. Replication of Beau-R was found to be restricted to soft tissue within the beak, whereas M41-CK was detected in beak tissue, trachea and eyelid up to seven days post infection. In vitro assays further identified that, unlike M41-CK, Beau-R could not replicate at 41 °C, the core body temperature of a chicken, but is able to replicate a 37 °C, a temperature relatable to the very upper respiratory tract. Using a panel of rIBVs with defined mutations in the structural and accessory genes, viral replication at permissive and non-permissive temperatures was investigated, identifying that the Beau-R replicase gene was a determinant of temperature sensitivity and that sub-genomic mRNA synthesis had been affected. The identification of temperature sensitive allelic lesions within the Beau-R replicase gene opens up the possibility of using this method of attenuation in other IBV strains for future vaccine development as well as a method to investigate the functions of the IBV replicase proteins.
has issue date	2020-07-14(xsd:dateTime)
bibo:doi	10.3390/v12070754
bibo:pmid	32674326
has license	cc-by
sha1sum (hex)	7d1d78403a919183f5349827f329c6e6bb7e678a
schema:url	https://doi.org/10.3390/v12070754
resource representing a document's title	Temperature Sensitivity: A Potential Method for the Generation of Vaccines against the Avian Coronavirus Infectious Bronchitis Virus
has PubMed Central identifier	PMC7412246
has PubMed identifier	32674326
schema:publication	Viruses
resource representing a document's body	covid:7d1d78403a919183f5349827f329c6e6bb7e678a#body_text
is schema:about of	named entity 'post' named entity 'molecular study' named entity 'M41' named entity 'pathogenic' named entity 'Temperature' named entity 'REPLICATE' named entity 'NATURAL' named entity 'ENABLE' named entity 'IS A' named entity 'DAYS' named entity 'GENOMIC' named entity 'DETERMINE' named entity 'identified' named entity 'replicase' named entity 'temperature' named entity 'beak' named entity 'enable' named entity 'possibility' named entity 'beak' named entity 'restricted' named entity 'molecular' named entity 'pathogenic' named entity 'temperature' named entity 'chickens' named entity 'recombinant' named entity 'future' named entity 'lesions' named entity 'defined' named entity 'Potential' named entity 'Virus' named entity 'molecular study' named entity 'gene' named entity 'IBV' named entity 'eyelid' named entity 'gene' named entity 'vaccine' named entity 'soft tissue' named entity 'vaccine' named entity 'vaccine' named entity 'IBV' named entity 'pathogens' named entity 'multiple comparisons' named entity 'hpi' named entity 'infection' named entity 'animal welfare' named entity 'phenotype' named entity 'MHV' named entity 'virions' named entity 'randomly selected' named entity 'randomly selected' named entity 'cell cultures' named entity 'virus' named entity 'upper respiratory' named entity 'leader sequence' named entity 'nucleocapsid' named entity 'highly contagious' named entity 'vaccine strain' named entity 'RT-PCR' named entity 'viruses' named entity 'replicase' named entity 'RT-PCR' named entity 'PFU' named entity 'PFU' named entity 'kidneys' named entity 'Roslin Institute' named entity 'hpi' named entity 'sgRNA' named entity 'IBV' named entity 'MHV' named entity 'hpi' named entity 'gene' named entity 'Supernatant'

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