About: In Preparation for Outdoor Pharming: Griffithsin Can Be Expressed in Nicotiana excelsiana and Retains Activity After Storage as Silage

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About: In Preparation for Outdoor Pharming: Griffithsin Can Be Expressed in Nicotiana excelsiana and Retains Activity After Storage as Silage Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	In Preparation for Outdoor Pharming: Griffithsin Can Be Expressed in Nicotiana excelsiana and Retains Activity After Storage as Silage
Creator	Fuqua, Joshua Palmer, Kenneth George', Felix Buyel, Johannes Cates, Jennifer Eapen, Paul Audrey, Yi-Hui Engineering, Bioprocess Mundell, Rich Teh, St Yao, Changhong
Source	Medline; PMC
abstract	Griffithsin is an algae-derived lectin with strong anti-viral activity against HIV and a positive safety profile. Multiple clinical studies are investigating griffithsin's utility as topical HIV microbicide. HIV microbicides are an extremely cost-sensitive market and plant-based griffithsin protein expression has the potential to meet those demands. The griffithsin product used in the clinic has been expressed and purified in N. benthamiana, using a TMV-based viral vector system, Geneware®. Outdoor pharming of biopharmaceuticals would further alleviate startup costs for biotechnology firms and may allow broader product accessibility. Therefore, this study assessed expression in a hybrid tobacco line, N. excelsiana, that is susceptible to TMV-based viral vectors and can be grown outdoors. In addition to using this hybrid line we expand on methods for in planta storage of griffithsin in leafy plants by ensiling kilogram quantities of griffithsin. The ensiling process allows year-round biomanufacturing, minimal environmental-controlled storage, and reduces the industry need for multiple growth areas to maintain multi-product manufacturing of plant-based pharmaceuticals. This study shows that griffithsin can be expressed in N. excelsiana and is stable, recoverable, and active from ensiled tissue. These studies can pave the way for future plant-based pharmaceuticals to be expressed and stored in this manner.
has issue date	2020-03-18(xsd:dateTime)
bibo:doi	10.3389/fbioe.2020.00199
bibo:pmid	32258012
has license	cc-by
sha1sum (hex)	7cc307de662b398dd5d5533a4781f775804242c5
schema:url	https://doi.org/10.3389/fbioe.2020.00199
resource representing a document's title	In Preparation for Outdoor Pharming: Griffithsin Can Be Expressed in Nicotiana excelsiana and Retains Activity After Storage as Silage
has PubMed Central identifier	PMC7093594
has PubMed identifier	32258012
schema:publication	Front Bioeng Biotechnol
resource representing a document's body	covid:7cc307de662b398dd5d5533a4781f775804242c5#body_text
is schema:about of	named entity 'ACTIVITY' named entity 'IN PREPARATION' named entity 'USING' named entity 'LINE' named entity 'PLANT-' named entity 'CONTROLLED' named entity 'ENVIRONMENTAL' named entity 'KILOGRAM' named entity 'NEED FOR' named entity 'PROCESS' named entity 'DEMANDS' named entity 'THESE' named entity 'COST' named entity 'EXTREMELY' named entity 'MULTIPLE' named entity 'ASSESSED' named entity 'MICROBICIDES' named entity 'BASED' named entity 'EXPRESSED' named entity 'STORAGE' named entity 'SENSITIVE' named entity 'TMV' named entity 'PLANTS' named entity 'TOBACCO' named entity 'BIOPHARMACEUTICALS' named entity 'INVESTIGATING' named entity 'YEAR' named entity 'STUDIES' named entity 'VIRAL VECTOR' named entity 'PROFILE' named entity 'BIOTECHNOLOGY' named entity 'SAFETY' named entity 'TISSUE' named entity 'STABLE' named entity 'POSITIVE' named entity 'EXPAND' named entity 'SUSCEPTIBLE' named entity 'CLINICAL STUDIES' named entity 'ACTIVE' named entity 'GROWTH' named entity 'MANUFACTURING' named entity 'IN PLANTA' named entity 'EXPRESSED' named entity 'PRODUCT' named entity 'LECTIN' named entity 'OUTDOORS' named entity 'MINIMAL' named entity 'FIRMS' named entity 'METHODS' named entity 'DERIVED' named entity 'PRODUCT ACCESSIBILITY' named entity 'WAY' named entity 'SILAGE' named entity 'POTENTIAL' named entity 'OUTDOOR' named entity 'HYBRID' named entity 'CLINIC' named entity 'STRONG' named entity 'MEET' named entity 'TO MAINTAIN' named entity 'STUDY' named entity 'TOPICAL' named entity 'ALLOW' named entity 'MARKET' named entity 'VIRAL VECTORS' named entity '27S' named entity 'FUTURE' named entity 'PURIFIED' named entity 'STORAGE' named entity 'RETAINS' named entity 'USED'

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