About: Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction

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About: Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction
Creator	Tsai, Yun-Long Lee, Fu-Chun Lee, Pei-Yu Chang, Hsiao-Fen Wang, Hwa-Tang Wilkes, Rebecca
Source	Medline; PMC
abstract	BACKGROUND: Canine distemper virus (CDV) has been associated with outbreaks of canine infectious respiratory disease in shelters and boarding kennel environments. POCKIT(TM) Nucleic Acid Analyzer is a field-deployable device capable of generating automatically interpreted insulated isothermal polymerase chain reaction (iiPCR) results from extracted nucleic acid within one hour. In this study, reverse transcription iiPCR (RT-iiPCR) was developed to facilitate point-of-need diagnosis of CDV infection. RESULTS: Analytical sensitivity (limit of detection 95%) of the established CDV RT-iiPCR was about 11 copies of in vitro transcribed RNA per reaction. CDV RT-iiPCR generated positive signals from CDV, but not Bordetella bronchiseptica, canine parvovirus, canine herpesvirus, canine adenovirus 2, canine influenza virus (subtype H3N8), canine parainfluenza virus, and canine respiratory coronavirus. To evaluate accuracy of the established reaction in canine distemper clinical diagnosis, 110 specimens from dogs, raccoons, and foxes suspected with CDV infection were tested simultaneously by CDV RT-iiPCR and real-time RT-PCR. CDV RT-iiPCR demonstrated excellent sensitivity (100%) and specificity (100%), compared to real-time RT-PCR. CONCLUSIONS: The results indicated an excellent correlation between RT-iiPCR and a reference real time RT-PCR method. Working in a lyophilized format, the established method has great potential to be used for point-of-care diagnosis of canine distemper in animals, especially in resource-limited facilities.
has issue date	2014-09-09(xsd:dateTime)
bibo:doi	10.1186/s12917-014-0213-8
bibo:pmid	25200113
has license	cc-by
sha1sum (hex)	7c1b1700315cc018b94e1488667365315137cb20
schema:url	https://doi.org/10.1186/s12917-014-0213-8
resource representing a document's title	Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction
has PubMed Central identifier	PMC4172905
has PubMed identifier	25200113
schema:publication	BMC Vet Res
resource representing a document's body	covid:7c1b1700315cc018b94e1488667365315137cb20#body_text
is schema:about of	named entity 'RAPID' named entity 'reaction' named entity 'canine' named entity 'raccoons' named entity 'Nucleic Acid' covid:arg/7c1b1700315cc018b94e1488667365315137cb20 named entity 'OUTBREAKS' named entity 'RNA' named entity 'SHELTERS' named entity 'ABOUT' named entity 'STUDY' named entity 'SPECIMENS' named entity 'DIAGNOSIS' named entity 'CLINICAL DIAGNOSIS' named entity 'DEVICE' named entity '110' named entity 'CANINE RESPIRATORY CORONAVIRUS' named entity 'GENERATED' named entity 'ESTABLISHED' named entity 'POSITIVE' named entity 'BACKGROUND' named entity 'CANINE DISTEMPER VIRUS' named entity 'FIELD' named entity 'IS A' named entity 'IN VITRO' named entity 'AUTOMATICALLY' named entity 'CAPABLE' named entity 'EVALUATE' named entity 'DOGS' named entity 'ASSOCIATED WITH' named entity 'BUT' named entity 'INFECTION' named entity 'COMPARED' named entity 'GENERATING' named entity 'REACTION' named entity 'NEED' named entity 'RESPIRATORY DISEASE' named entity 'REVERSE TRANSCRIPTION' named entity 'DEMONSTRATED' named entity 'COPIES' named entity 'FOXES' named entity 'BORDETELLA BRONCHISEPTICA' named entity 'EXTRACTED' named entity 'TRANSCRIBED' named entity 'RESULTS' named entity 'TESTED' named entity 'ONE HOUR' named entity 'DETECTION' named entity 'CANINE DISTEMPER VIRUS' named entity 'ACCURACY' named entity 'INFECTIOUS' named entity 'RACCOONS' named entity 'TUBE' named entity 'ENVIRONMENTS' named entity 'ANALYTICAL' named entity 'SENSITIVITY' named entity 'DETECTION' named entity 'CANINE DISTEMPER' named entity 'POLYMERASE CHAIN REACTION' named entity 'CANINE ADENOVIRUS 2' named entity 'NUCLEIC ACID' named entity 'INTERPRETED' named entity 'CANINE PARAINFLUENZA VIRUS' named entity 'CANINE' named entity 'SENSITIVE' named entity 'POLYMERASE CHAIN REACTION' named entity 'EXCELLENT' named entity 'OF-' named entity 'SUSPECTED' named entity 'SIMULTANEOUSLY' named entity '28100' named entity 'CANINE PARVOVIRUS' named entity 'REAL-TIME RT-PCR' named entity 'REVERSE TRANSCRIPTION' named entity 'CDV' named entity 'CANINE HERPESVIRUS'

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