About: Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions

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About: Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions
Creator	Herrler, Georg Meng, Fandan Valentin-Weigand, Peter Wu, Nai-Huei Seitz, Maren
Source	PMC
abstract	Streptococci may colonize the epithelium in the airways and other entry sites. While local infection often remains asymptomatic, severe or even fatal diseases occur when streptococci become invasive and spread to different sites in the infected host. We have established porcine respiratory air-liquid interface cultures (ALI) from the porcine lung to analyze the interaction of streptococci with their primary target cells. As representative of the streptococcal family we chose Streptococcus suis (S. suis) that is not only a major swine respiratory pathogen but can also infect humans. Suilysin, a cholesterol-dependent cytolysin (CDC), is an important virulence factor. By comparing a S. suis wt strain with a suilysin-deficient mutant, we demonstrate that suilysin contributes to (i) adherence to airway cells (ii) loss of ciliated cells (iii) apoptosis, and (iv) invasion. Furthermore, we show that cytolytic activity of suilysin is crucial for these effects. A striking result of our analysis was the high efficiency of S. suis-induced apoptosis and invasion upon infection under ALI conditions. These properties have been reported to be less efficient when analyzed with immortalized cells. We hypothesize that soluble effectors such as suilysin are present at higher concentrations in cells kept at ALI conditions and thus more effective. These results should be relevant also for infection of the respiratory tract by other respiratory pathogens.
has issue date	2016-05-27(xsd:dateTime)
bibo:doi	10.1038/srep26748
bibo:pmid	27229328
has license	cc-by
sha1sum (hex)	7c0168a4b858a8680f305fc4d8cc3c787ff4e110
schema:url	https://doi.org/10.1038/srep26748
resource representing a document's title	Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions
has PubMed Central identifier	PMC4882623
has PubMed identifier	27229328
schema:publication	Sci Rep
resource representing a document's body	covid:7c0168a4b858a8680f305fc4d8cc3c787ff4e110#body_text
is schema:about of	named entity 'MEDIATED' named entity 'CONDITIONS' named entity 'RESPIRATORY' named entity 'ALI' named entity 'major' named entity 'While' named entity 'concentrations' named entity 'hypothesize' named entity 'comparing' named entity 'invasive' named entity 'family' named entity 'These' named entity 'STREPTOCOCCAL INFECTION' named entity 'CYTOLYSIN' named entity 'SITES' named entity 'ANALYZED' named entity 'OUR' named entity 'INFECT' named entity 'INTERACTION' named entity 'PRIMARY' named entity 'ADHERENCE' named entity 'RESPIRATORY TRACT' named entity 'INFECTED HOST' named entity 'HAVE' named entity 'EPITHELIUM' named entity 'DEFICIENT' named entity 'SUILYSIN' named entity 'THEIR' named entity 'HUMANS' named entity 'INFECTION' named entity 'PATHOGEN' named entity 'DIFFERENT' named entity 'COMPARING' named entity 'MUTANT' named entity 'SWINE' named entity 'A MAJOR' named entity 'CILIATED CELLS' named entity '28S' named entity 'RESPIRATORY PATHOGENS' named entity 'LOCAL INFECTION' named entity 'RESPIRATORY' named entity 'RELEVANT' named entity 'IMPORTANT' named entity 'INDUCED' named entity 'TARGET CELLS' named entity '28I' named entity 'LUNG' named entity 'REPRESENTATIVE' named entity 'RESULT' named entity 'HIGHER' named entity 'OCCUR' named entity 'STREPTOCOCCUS SUIS' named entity 'RESPIRATORY AIR' named entity 'EFFECTS' named entity 'INVASIVE' named entity 'ALI' named entity 'VIRULENCE FACTOR' named entity 'LOSS OF' named entity 'BUT' named entity 'SUILYSIN' named entity 'CULTURES' named entity 'CHOLESTEROL' named entity 'HIGH EFFICIENCY' named entity 'STREPTOCOCCI' named entity 'CONDITIONS' named entity 'SEVERE' named entity 'FATAL' named entity 'PRESENT' named entity 'DEPENDENT' named entity 'OPEN' named entity 'EFFICIENT' named entity 'INTERFACE' named entity 'AIR-LIQUID' named entity 'PORCINE' named entity 'EPITHELIAL CELLS' named entity 'AIR-LIQUID' named entity 'REPORTED'

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