About: Identification of Potassium and Calcium Channel Inhibitors as Modulators of Polyomavirus Endosomal Trafficking

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Identification of Potassium and Calcium Channel Inhibitors as Modulators of Polyomavirus Endosomal Trafficking Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of Potassium and Calcium Channel Inhibitors as Modulators of Polyomavirus Endosomal Trafficking
Creator	Whitehouse, Adrian Mankouri, Jamel Dobson, Samuel
Source	Elsevier; Medline; PMC
abstract	Abstract During virus entry, members of the Polyomaviridae transit the endolysosomal network en route to the endoplasmic reticulum (ER), from which degraded capsids escape into the cytoplasm and enter the nucleus. Emerging evidence suggests that viruses require both endosomal acidification and the correct ionic balance of K+ and Ca2+ ions in endosomes for correct virus trafficking and genome release. Here, using two polyomaviruses with different capsid architectures, namely Simian virus 40 (SV40) and Merkel cell polyomavirus (MCPyV), we describe methods to rapidly quantify virus infection using IncuCyte ZOOM imaging analysis, and use this system to investigate the role of both K+ and Ca2+ channels during the early stages of virus entry. Using broad spectrum blockers of both K+ and Ca2+ channels to specifically target host cell ion channel functionality, we show that MCPyV, but not SV40 can be inhibited by K+ channel modulators, whilst both viruses are restricted by the broad spectrum Ca2+ channel inhibitor verapamil. Using a panel of more specific Ca2+ blockers, we show that both MCPyV and SV40 are dependent on the activity of two-pore Ca2+ channels (TPCs), as the TPC-specific blocker tetrandrine could prevent the capsid disassembly and nuclear transport required for virus entry. We therefore reveal a novel target to restrict the entry of polyomaviruses, which given the known role of TPCs during endolysosomal-ER fusion, is likely to be applicable to other viruses that transit this pathway.
has issue date	2020-05-08(xsd:dateTime)
bibo:doi	10.1016/j.antiviral.2020.104819
bibo:pmid	32389733
has license	els-covid
sha1sum (hex)	7b5230c98f4d93bd25f5f53dd3dd235ec977dfd6
schema:url	https://doi.org/10.1016/j.antiviral.2020.104819
resource representing a document's title	Identification of Potassium and Calcium Channel Inhibitors as Modulators of Polyomavirus Endosomal Trafficking
has PubMed Central identifier	PMC7205714
has PubMed identifier	32389733
schema:publication	Antiviral Res
resource representing a document's body	covid:7b5230c98f4d93bd25f5f53dd3dd235ec977dfd6#body_text
is schema:about of	named entity 'Emerging' named entity 'capsids' named entity 'During' named entity 'nuclear transport' named entity 'Inhibitors' named entity 'Channel' named entity 'Channel' named entity 'Potassium' named entity 'VERAPAMIL' named entity 'DEGRADED' named entity 'EARLY STAGES' named entity 'CORRECT' named entity 'EVIDENCE' named entity 'INHIBITOR' named entity 'REQUIRED' named entity 'PORE' named entity 'MERKEL CELL POLYOMAVIRUS' named entity 'prevent' named entity 'virus' named entity 'virus' named entity 'channels' named entity 'channel' named entity 'broad' named entity 'endosomes' named entity 'channel' named entity 'SV40' named entity 'antibody' named entity 'Connecticut' named entity 'Alfa Aesar' named entity 'Thermo Fisher Scientific' named entity 'escape' named entity 'functionality' named entity 'capsid' named entity 'Calcium' named entity 'Trafficking' named entity 'polyomaviruses' named entity 'virus entry' named entity 'endosomal' named entity 'Polyomaviridae' named entity 'verapamil' named entity 'SV40' named entity 'virus' named entity 'virus entry' named entity 'cytoplasm' named entity 'nucleus' named entity 'viruses' named entity 'capsid' named entity 'Endosomal Trafficking' named entity 'Calcium Channel' named entity 'Polyomavirus' named entity 'Polyomavirus' named entity 'Potassium' named entity 'virus' named entity 'capsid proteins' named entity 'negative sense RNA' named entity 'antibody' named entity 'SV40' named entity 'virions' named entity 'Abcam' named entity 'virus entry' named entity 'verapamil' named entity 'DAPI' named entity 'clade' named entity 'verapamil' named entity 'Schumann' named entity 'virions' named entity 'Virions' named entity 'DMEM' named entity 'K V' named entity 'pentamer' named entity 'nucleus' named entity 'National Cancer Institute' named entity 'nitrendipine' named entity 'T-antigen' named entity 'SV40' named entity 'NAADP' named entity '4AP' named entity 'Nitrendipine' named entity '4-aminopyridine'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software