About: Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli 1 1 Edited by M. Gottesman

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About: Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli 1 1 Edited by M. Gottesman Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli 1 1 Edited by M. Gottesman
Creator	Anderson, J Magliery, Thomas Schultz, Peter
Source	Elsevier; Medline; PMC
abstract	Abstract Naturally occurring tRNA mutants are known that suppress +1 frameshift mutations by means of an extended anticodon loop, and a few have been used in protein mutagenesis. In an effort to expand the number of possible ways to uniquely and efficiently encode unnatural amino acids, we have devised a general strategy to select tRNAs with the ability to suppress four-base codons from a library of tRNAs with randomized 8 or 9 nt anticodon loops. Our selectants included both known and novel suppressible four-base codons and resulted in a set of very efficient, non-cross-reactive tRNA/four-base codon pairs for AGGA, UAGA, CCCU and CUAG. The most efficient four-base codon suppressors had Watson-Crick complementary anticodons, and the sequences of the anticodon loops outside of the anticodons varied with the anticodon. Additionally, four-base codon reporter libraries were used to identify “shifty” sites at which +1 frameshifting is most favorable in the absence of suppressor tRNAs in Escherichia coli. We intend to use these tRNAs to explore the limits of unnatural polypeptide biosynthesis, both in vitro and eventually in vivo. In addition, this selection strategy is being extended to identify novel five- and six-base codon suppressors.
has issue date	2001-03-30(xsd:dateTime)
bibo:doi	10.1006/jmbi.2001.4518
bibo:pmid	11273699
has license	els-covid
sha1sum (hex)	7a0a75a2fa75bb82ef40a3806b2178eee7153618
schema:url	https://doi.org/10.1006/jmbi.2001.4518
resource representing a document's title	Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli 1 1 Edited by M. Gottesman
has PubMed Central identifier	PMC7125544
has PubMed identifier	11273699
schema:publication	Journal of Molecular Biology
resource representing a document's body	covid:7a0a75a2fa75bb82ef40a3806b2178eee7153618#body_text
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