About: Quantitation of canine coronavirus RNA in the faeces of dogs by TaqMan RT-PCR

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About: Quantitation of canine coronavirus RNA in the faeces of dogs by TaqMan RT-PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Quantitation of canine coronavirus RNA in the faeces of dogs by TaqMan RT-PCR
Creator	Buonavoglia, Canio Campolo, Marco Decaro, Nicola Elia, Gabriella Martella, Vito Tempesta, Maria Pratelli, Annamaria
Source	Elsevier; Medline; PMC
abstract	A TaqMan(®) fluorogenic reverse transcriptase-polymerase chain reaction (RT-PCR) assay was developed for the detection and quantitation of canine coronavirus (CCoV) RNA in the faeces of naturally or experimentally infected dogs. The CCoV fluorogenic RT-PCR assay, which targeted the ORF5 (M gene), was more sensitive than a conventional RT-PCR assay targeting the same gene, showing a detection limit of 10 copies of CCoV standard RNA, and was linear from 10 to 10(8) copies, allowing quantitation of samples with a wide range of CCoV RNA loads. A total of 78 faecal samples of diarrhoeic dogs were tested simultaneously by conventional and fluorogenic RT-PCR: 29 were negative by both techniques, whereas 27 tested positive by conventional RT-PCR and 48 by the established CCoV fluorogenic assay. One sample, which was positive by conventional RT-PCR, gave no signal in the fluorogenic assay. In addition, by the fluorogenic assay CCoV shedding in the faecal samples of an experimentally infected dog was monitored for 28 days. The high sensitivity, simplicity and reproducibility of the CCoV fluorogenic RT-PCR assay, combined with its wide dynamic range and high throughput, make this method especially suitable for efficacy trials on CCoV vaccines.
has issue date	2004-05-07(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2004.03.012
bibo:pmid	15158596
has license	no-cc
sha1sum (hex)	79d49888e65deed3a6fc0ca8c7deda2a1528cb87
schema:url	https://doi.org/10.1016/j.jviromet.2004.03.012
resource representing a document's title	Quantitation of canine coronavirus RNA in the faeces of dogs by TaqMan RT-PCR
has PubMed Central identifier	PMC7119844
has PubMed identifier	15158596
schema:publication	J Virol Methods
resource representing a document's body	covid:79d49888e65deed3a6fc0ca8c7deda2a1528cb87#body_text
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