About: Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
Creator	Beth-Din, Adi Israeli, Ofir Laskar, Orly Paran, Nir Stein, Dana Yitzhaki, Shmuel Atiya-Nasagi, Yafit Weiss, Shay Lazar, Shirley Milrot, Elad Schuster, Ofir
Source	Elsevier; Medline; PMC
abstract	Abstract SARS-CoV-2 genetic identification is based on viral RNA extraction prior to RT-qPCR assay, however recent studies support the elimination of the extraction step. Herein, we assessed the RNA extraction necessity, by comparing RT-qPCR efficacy in several direct approaches vs. the gold standard RNA extraction, in detection of SARS-CoV-2 from laboratory samples as well as clinical Oro-nasopharyngeal SARS-CoV-2 swabs. Our findings show advantage for the extraction procedure, however a direct no-buffer approach might be an alternative, since it identified more than 60% of positive clinical specimens.
has issue date	2020-08-10(xsd:dateTime)
bibo:doi	10.1016/j.ijid.2020.08.015
bibo:pmid	32791207
has license	els-covid
sha1sum (hex)	798fe10b45aa1f13170eaf647b4bab000af7e60c
schema:url	https://doi.org/10.1016/j.ijid.2020.08.015
resource representing a document's title	Evaluating the efficacy of RT-qPCR SARS-CoV-2 direct approaches in comparison to RNA extraction
has PubMed Central identifier	PMC7416699
has PubMed identifier	32791207
schema:publication	Int J Infect Dis
resource representing a document's body	covid:798fe10b45aa1f13170eaf647b4bab000af7e60c#body_text
is schema:about of	named entity 'support' named entity 'SARS-CoV-2' named entity 'direct' named entity 'ELIMINATION' covid:arg/798fe10b45aa1f13170eaf647b4bab000af7e60c named entity 'genetic' named entity 'RNA extraction' named entity 'buffers' named entity 'Journal' named entity 'RT-qPCR' named entity 'SARS-CoV-2' named entity 'lysis' named entity 'reagent' named entity 'titer' named entity 'RNA extraction' named entity 'SARS-CoV-2' named entity 'diagnostic procedure' named entity 'gene' named entity 'COVID-19 pandemic' named entity 'RNA extraction' named entity 'RT-qPCR' named entity 'RT-qPCR' named entity 'COVID-19' named entity 'RNA extraction' named entity 'reverse transcriptase quantitative PCR' named entity 'RNA extraction' named entity 'SARS-CoV-2' named entity 'funding source' named entity 'supply chains' named entity 'lysis' named entity 'clinical diagnosis' named entity 'SARS-CoV-2' named entity 'lysis' named entity 'morbidity' named entity 'RNA isolation' named entity 'RT-qPCR' named entity 'nucleic acids' named entity 'clinical samples' named entity 'nasopharyngeal swabs' named entity 'clinical samples' named entity 'Berlin' named entity 'RNA extraction' named entity 'SARS-CoV-2' named entity 'RNA extraction' named entity 'SARS-CoV-2' named entity 'RNA extraction' named entity 'RT-qPCR' named entity 'RT-qPCR' named entity 'assay' named entity 'RNA extraction' named entity 'IDENTIFICATION' named entity 'STUDIES' named entity 'SARS-COV-2' named entity 'direct' named entity 'viral' named entity 'RT-qPCR' named entity 'RT-qPCR' named entity 'efficacy' named entity 'EXTRACTION' named entity 'PRIOR TO' named entity 'extraction' named entity 'assay' named entity 'RNA extraction' named entity 'STEP' named entity 'step' named entity 'studies' named entity 'SARS-CoV-2' named entity 'RNA extraction' named entity 'APPROACHES' named entity 'elimination' named entity 'direct'

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