About: Sodium-dependent glucose transporter 1 and glucose transporter 2 mediate intestinal transport of quercetrin in Caco-2 cells

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About: Sodium-dependent glucose transporter 1 and glucose transporter 2 mediate intestinal transport of quercetrin in Caco-2 cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Sodium-dependent glucose transporter 1 and glucose transporter 2 mediate intestinal transport of quercetrin in Caco-2 cells
Source	PMC
abstract	BACKGROUND: The role of glucose transporters in the transport of flavonoids remains ambiguous. OBJECTIVE: In this study, we examined whether quercitrin would be absorbed intactly in modeled Caco-2 cells, as well as determined the involvement of sodium-dependent glucose transporter 1 (SGLT1) and glucose transporter 2 (GLUT2) in its transmembrane transport. DESIGN: The first experiment was conducted to examine the uptake of quercitrin into Caco-2 cells 24 h after they were seeded and the second experiment was conducted to determine the transport across the apical and basolateral membrane of Caco-2 cells after they were cultured for 21 days in a Millicell system. Quercitrin was administered at 3, 9, or 18 μg/mL; and the time points of sampling were 30, 60, 90, 120, and 150 min. RESULTS: In the uptake experiment, the highest intracellular quercitrin concentration was observed in the cells treated with 18 μg/mL quercitrin at 60 min, with a bell-shaped kinetic curve. Quercetin, isorhamnetin, and tamarixetin were detected inside the cells, particularly when treated with a high dose. In the transport experiment, quercitrin was transported from the apical to basolateral side and vice versa; its concentrations depended on dose, time, and transport direction (P < 0.0001). Only trace amounts of isorhamnetin and tamarixetin were detected in the apical chamber when quercitrin was added to the basolateral chamber. Phloridzin and phloretin, potent inhibitors of SGLT1 and GLUT2, respectively, significantly diminished quercitrin transport from the apical to basolateral side; and phloretin had a greater inhibitory effect compared to phloridzin. CONCLUSION: Our results demonstrate that quercitrin is absorbed intactly and then effluxed out of Caco-2 cells through both apical and basolateral membranes probably via SGLT1 and GLUT2.
has issue date	2020-06-15(xsd:dateTime)
bibo:doi	10.29219/fnr.v64.3745
has license	cc-by
sha1sum (hex)	79110063c821e4039a257ca9a30d0f965a2eba1e
schema:url	https://doi.org/10.29219/fnr.v64.3745
resource representing a document's title	Sodium-dependent glucose transporter 1 and glucose transporter 2 mediate intestinal transport of quercetrin in Caco-2 cells
has PubMed Central identifier	PMC7307431
schema:publication	Food Nutr Res
resource representing a document's body	covid:79110063c821e4039a257ca9a30d0f965a2eba1e#body_text
is schema:about of	named entity 'quercetin' named entity 'transmembrane' named entity 'elution' named entity 'quercetrin' named entity 'quercetin' named entity 'flavonoid glycosides' named entity 'glycoside' named entity 'quercitrin' named entity 'Manassas' named entity 'm/z' named entity 'Santa Clara, CA' named entity 'apical membrane' named entity 'tamarixetin' named entity 'quercetin' named entity 'cyanidin-3-glucoside' named entity 'diarrhea' named entity 'glycoside' named entity 'Quercitrin' named entity 'methylation' named entity 'intestine' named entity 'SGLT1' named entity 'tissue culture' named entity 'quercetin' named entity 'tea' named entity 'phloridzin' named entity 'methylated' named entity 'moiety' named entity 'quercetin' named entity 'SPSS' named entity 'bioavailability' named entity 'glycosides' named entity 'methylation' named entity 'trypsin' named entity 'tamarixetin' named entity 'quercitrin' named entity 'non-specific' named entity 'P-value' named entity 'health benefits' named entity 'quercitrin' named entity 'rhamnose' named entity 'phlorizin' named entity 'flavonoids' named entity 'glucosides' named entity 'quercitrin' named entity 'phase II enzymes' named entity 'flavonoid' named entity 'internal standard' named entity 'tamarixetin' named entity 'metabolism' named entity 'capillary' named entity 'ATPase' named entity 'MTT assay' named entity 'flavonoid glycoside' named entity 'Bonferroni adjustment' named entity 'plant extracts' named entity 'type 2 diabetes' named entity 'quercitrin' named entity 'MPB' named entity 'potency' named entity 'MPB' named entity 'rat' named entity 'glucose' named entity 'rat' named entity 'SGLT1' named entity 'quercitrin' named entity 'Flavonoids' named entity 'Caco-2' named entity 'quercitrin' named entity 'quercitrin' named entity 'lactase' named entity 'quercitrin' named entity 'Logan, UT' named entity 'quercitrin' named entity 'phloretin' named entity 'streptomycin' named entity 'quercitrin' named entity 'UDP-glucuronosyl transferase' named entity 'amino acids' named entity 'supernatant' named entity 'quercitrin' named entity 'Caco-2' named entity 'isoquercitrin' named entity 'quercetin' named entity 'Phloridzin'

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