About: Cystatin D, a natural salivary cysteine protease inhibitor, inhibits coronavirus replication at its physiologic concentration

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About: Cystatin D, a natural salivary cysteine protease inhibitor, inhibits coronavirus replication at its physiologic concentration Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cystatin D, a natural salivary cysteine protease inhibitor, inhibits coronavirus replication at its physiologic concentration
Creator	Collins, Arlene Collins', A Grubb2, A
Source	Medline; PMC
abstract	This study was conducted to examine the effect of cystatin D, a newly discovered salivary cysteine protease inhibitor, on human coronavirus replication. When MRC‐5, human diploid lung cells, were incubated with dilutions of recombinant human cystatin D from 0.65–10 μM for 1 h prior to, during and after infection with coronavirus OC43 and 229e strains, a decrease in virus yield was observed resulting in an IC(50) of 0.8 μM for both virus strains. This dose is within the normal concentration range of cystatin D, 0.12–1.9 μM found in saliva. When a single dose, 2.5 μM, was applied, cystatin inhibition of release of virus progeny was not overcome until three days post infection whereas inhibition by leupeptin, a serine and cysteine protease inhibitor, was completely abrogated by two days. When cellular toxicity was measured by (3)H‐thymidine uptake, cystatin D did not markedly affect cell proliferation below a 10 μM dose. The results demonstrate that cystatin D is a potent inhibitor of coronavirus replication.
has issue date	2008-06-28(xsd:dateTime)
bibo:doi	10.1111/j.1399-302x.1998.tb00753.x
bibo:pmid	9573825
has license	no-cc
sha1sum (hex)	78c46f5f9e95e60743735aa0f42b9a24dd3bb5c3
schema:url	https://doi.org/10.1111/j.1399-302x.1998.tb00753.x
resource representing a document's title	Cystatin D, a natural salivary cysteine protease inhibitor, inhibits coronavirus replication at its physiologic concentration
has PubMed Central identifier	PMC7167923
has PubMed identifier	9573825
schema:publication	Oral Microbiol Immunol
resource representing a document's body	covid:78c46f5f9e95e60743735aa0f42b9a24dd3bb5c3#body_text
is schema:about of	named entity 'inhibits' named entity 'concentration' named entity 'ORAL' named entity 'SALIVARY' covid:arg/78c46f5f9e95e60743735aa0f42b9a24dd3bb5c3 named entity 'concentration' named entity 'Munksgaard' named entity 'Lund' named entity 'cysteine protease inhibitor' named entity 'Sweden' named entity 'cystatin' named entity 'active site' named entity 'cerebrospinal fluid' named entity 'cystatin' named entity 'recombinant' named entity 'cysteine protease' named entity 'cystatin' named entity 'cell growth' named entity 'Eagle's minimum essential medium' named entity 'cystatin' named entity 'antiviral effect' named entity 'polioviruses' named entity 'virus' named entity 'protease' named entity 'virus' named entity 'intracellular' named entity 'infection' named entity 'proteases' named entity 'proteasome' named entity 'Minnetonka' named entity 'harmful effects' named entity 'leupeptin' named entity 'release of virus' named entity 'open reading frames' named entity 'neutral red' named entity 'human coronaviruses' named entity 'saliva' named entity 'infection' named entity 'cystatin' named entity 'Inhibitory activity' named entity 'cystatin' named entity 'cellular uptake' named entity 'cathepsins' named entity 'leupeptin' named entity '37°C' named entity 'E. coli' named entity 'infection' named entity 'MRC-5' named entity 'cathepsin' named entity 'oral route' named entity 'proteinase inhibitors' named entity 'cystatin' named entity 'virus' named entity 'intracellular' named entity 'virus' named entity 'acute gastroenteritis' named entity 'assay' named entity 'supernatant' named entity 'release of virus' named entity 'carbon atom' named entity 'Leupeptin' named entity 'multiplicity of infection' named entity 'cystatin' named entity 'assay' named entity 'Cystatin' named entity 'leupeptin' named entity 'herpesvirus' named entity 'cystatin' named entity 'Coronavirus infections' named entity 'active site' named entity 'inoculum' named entity 'virus' named entity 'incubation' named entity 'adsorption' named entity 'virions' named entity 'Escherichia coli' named entity 'leupeptin' named entity 'protease' named entity 'repressor'

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