About: Determination of the cell tropism of serotype 1 feline infectious peritonitis virus using the spike affinity histochemistry in paraffin‐embedded tissues

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About: Determination of the cell tropism of serotype 1 feline infectious peritonitis virus using the spike affinity histochemistry in paraffin‐embedded tissues Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Determination of the cell tropism of serotype 1 feline infectious peritonitis virus using the spike affinity histochemistry in paraffin‐embedded tissues
Creator	Chang, Hui-Wen Jeng, Chian-Ren Chen, Hui-Wen Chang, Yen-Chen Tsai, Pei-Shiue Wu, Ching-Ho Pang, Victor Cham, Tat-Chuan
source	Medline; PMC
abstract	Unlike for serotype II feline coronaviruses (FCoV II), the cellular receptor for serotype I FCoV (FCoV I), the most prevalent FCoV serotype, is unknown. To provide a platform for assessing the pattern by which FCoV I attaches to its host receptor(s), HEK293 cell lines that stably express the ectodomains of the spike (S) proteins derived from a FCoV I feline enteric coronavirus strain UU7 (FECV UU7) and a feline infectious peritonitis virus strain UU4 (FIPV UU4) were established. Using the recombinant S proteins as probes to perform S protein affinity histochemistry in paraffin‐embedded tissues, although no tissue or enteric binding of FECV UU7 S protein was detected, it was found that by immunohistochemistry that the tissue distribution of FIPV UU4 S protein‐bound cells correlated with that of FIPV antigen‐positive cells and lesions associated with FIP and that the affinity binding of FIPV UU4 S protein on macrophages was not affected by enzymatic removal of host cell‐surface sialic acid with neuraminidase. These findings suggest that a factor(s) other than sialic acid contribute(s) to the macrophage tropism of FIPV strain UU4. This approach allowed obtaining more information about both virus–host cell interactions and the biological characteristics of the unidentified cellular receptor for FCoV I.
has issue date	2017-08-16(xsd:dateTime)
bibo:doi	10.1111/1348-0421.12498
bibo:pmid	28675506
has license	no-cc
sha1sum (hex)	777d36ef204f5e963619a0efd3348a41b67b4dc4
schema:url	https://doi.org/10.1111/1348-0421.12498
resource representing a document's title	Determination of the cell tropism of serotype 1 feline infectious peritonitis virus using the spike affinity histochemistry in paraffin‐embedded tissues
has PubMed Central identifier	PMC7168434
has PubMed identifier	28675506
schema:publication	Microbiol Immunol
resource representing a document's body	covid:777d36ef204f5e963619a0efd3348a41b67b4dc4#body_text
is schema:about of	named entity 'neuraminidase' named entity 'receptor' named entity 'feline' named entity 'receptor' named entity 'virus' named entity 'INTERACTIONS' named entity 'FOUND' named entity 'probes' named entity 'FIPV' named entity 'host' named entity 'coronaviruses' named entity 'lesions' named entity 'virus' named entity 'renal tubular cells' named entity 'FIPV' named entity 'tissue sections' named entity 'forward scatter' named entity 'protein' named entity 'subcloned' named entity 'enterocytes' named entity 'receptor' named entity 'cells' named entity 'tropism' named entity 'FIPV' named entity 'FIPV' named entity 'macrophages' named entity 'FIPV' named entity 'immunohistochemistry' named entity 'cell lines' named entity 'macrophages' named entity 'serotype' named entity 'affinity' named entity 'protein' named entity 'protein' named entity 'post-mortem' named entity 'protein' named entity 'antigen' named entity 'antibody' named entity 'coronaviruses' named entity 'blood vessels' named entity 'cellular receptor' named entity 'protein' named entity 'histochemistry' named entity 'FIPV' named entity 'FIPV' named entity 'protein' named entity 'Alphacoronavirus' named entity 'KDa' named entity 'Biological Sequences' named entity 'coronaviruses' named entity 'amino acid' named entity 'macrophages' named entity 'genetic characteristics' named entity 'ectodomain' named entity 'cell tropism' named entity 'restriction sites' named entity 'Life Technologies' named entity 'recombinant' named entity 'Qiagen' named entity 'histochemistry' named entity 'FIPV' named entity 'virus' named entity 'flow cytometry' named entity 'FIPV' named entity 'plasmid' named entity 'FCoV' named entity 'protein binding' named entity 'tissue tropism' named entity 'FIPV' named entity 'glycosylation' named entity 'pathogenicity' named entity 'macrophages' named entity 'host cells' named entity 'FIPV'

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