About: Diagnosis of canine coronavirus infection using nested-PCR

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Diagnosis of canine coronavirus infection using nested-PCR
Creator	Buonavoglia, Canio Martella, Vito Tempesta, Maria Pratelli, Annamaria Lavazza, Antonio Buonavoglia, Domenico
Source	Elsevier; Medline; PMC
abstract	The results of polymerase chain reaction (PCR) and nested polymerase chain reaction (n-PCR) assays for the diagnosis of canine coronavirus (CCV) infection, and the comparison with other diagnostic techniques, such as electron microscopy (EM) and virus isolation using A-72 cell line are reported. The study was carried out on 71 faecal samples of pups with enteritis. Of 71 samples examined 14 were positive in PCR, whereas 30 samples resulted positive in the n-PCR assay. CCV was detected by EM examination in only four out of 45 samples, and by virus isolation in three out of 30 samples n-PCR positive.
has issue date	1999-12-06(xsd:dateTime)
bibo:doi	10.1016/s0166-0934(99)00134-2
bibo:pmid	10644090
has license	no-cc
sha1sum (hex)	75f84a9ed3c18daf666889f8c0b3d4d9c87ca486
schema:url	https://doi.org/10.1016/s0166-0934%2899%2900134-2
resource representing a document's title	Diagnosis of canine coronavirus infection using nested-PCR
has PubMed Central identifier	PMC7119627
has PubMed identifier	10644090
schema:publication	J Virol Methods
resource representing a document's body	covid:75f84a9ed3c18daf666889f8c0b3d4d9c87ca486#body_text
is schema:about of	named entity 'PCR' named entity 'chain reaction' named entity 'chain reaction' named entity 'CORONAVIRUS INFECTION' named entity 'DIAGNOSIS' named entity 'DETECTED' named entity 'CANINE CORONAVIRUS' named entity 'A-72 CELL' named entity 'RESULTS' named entity 'USING' named entity 'ELECTRON MICROSCOPY' named entity 'CELL LINE' named entity 'VIRUS ISOLATION' named entity 'NESTED POLYMERASE CHAIN REACTION' named entity 'CANINE CORONAVIRUS' named entity 'DIAGNOSIS' named entity 'SAMPLES' named entity 'COMPARISON' named entity 'CARRIED' named entity 'NESTED' named entity 'STUDY' named entity 'INFECTION' named entity 'EXAMINED' named entity 'PCR POSITIVE' named entity 'EXAMINATION' named entity 'CCV' named entity 'POLYMERASE CHAIN REACTION' named entity 'ENTERITIS' named entity 'REPORTED' named entity 'DIAGNOSTIC TECHNIQUES' named entity 'FAECAL' named entity 'PUPS' named entity 'POSITIVE' named entity 'USING' named entity 'PCR' named entity 'diagnosis' named entity 'virus isolation' named entity 'infection' named entity 'assay' named entity 'PCR' named entity 'polymerase chain reaction' named entity 'antibodies' named entity 'enteritis' named entity 'antibodies' named entity 'virus' named entity 'supernatant' named entity 'Electron microscopic' named entity 'faecal samples' named entity 'Negative staining' named entity 'PCR' named entity 'AGA' named entity 'antisense' named entity 'Formvar' named entity 'centrifuged' named entity 'Coronaviridae' named entity 'Hyatt' named entity 'supernatant' named entity 'viral particles' named entity 'sensitive and specific' named entity 'transmembrane protein' named entity 'monospecific' named entity 'coronaviruses' named entity 'inoculated' named entity 'sodium' named entity 'faeces' named entity 'PCR' named entity 'PCR' named entity 'diagnostic tool' named entity 'faecal samples' named entity 'enteritis' named entity 'faecal samples' named entity 'pathogen' named entity 'virus isolation' named entity 'morphologically' named entity 'colloids' named entity 'virus isolation'

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