About: An electrochemical label-free DNA impedimetric sensor with AuNP-modified glass fiber/carbonaceous electrode for the detection of HIV-1 DNA

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About: An electrochemical label-free DNA impedimetric sensor with AuNP-modified glass fiber/carbonaceous electrode for the detection of HIV-1 DNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	An electrochemical label-free DNA impedimetric sensor with AuNP-modified glass fiber/carbonaceous electrode for the detection of HIV-1 DNA
Creator	Caglayan, · Yeter, Ece
Source	Medline; PMC
abstract	In this study, a highly sensitive, electrochemical, and label-free DNA impedimetric sensor was developed using carbonized glass fiber–coal tar pitch (GF–CTP) electrodes supported with gold nanoparticles (AuNPs) for the detection of HIV-1 gene. Thiol-modified GF–CTP electrodes were prepared using amine crosslinking chemistry and AuNPs were self-assembled obtaining highly conductive nanoelectrodes, GF–CTP–ATP–Au. All steps of electrode modifications were characterized using electrochemical, spectroscopic, and microscopic methods. GF–CTP–ATP–Au electrode was then modified with a capture DNA probe (C-ssDNA) and optimized with a target DNA probe in terms of hybridization time and temperature between 30 and 180 min and 20–50 °C, respectively. Finally, the analytic performance of the developed ssDNA biosensor was evaluated using electrochemical impedance spectroscopy. The calibration of the sensor was obtained between 0.1 pM and 10 nM analyte working range. The limit of detection was calculated using signal to noise ratio of 3 (S/N = 3) as 13 fM. Moreover, interference results for two noncomplementary DNA probes were also tested to demonstrate non-specific ssDNA interactions. An electrochemical label-free DNA impedimetric sensor was successfully developed using a novel GF–CTP–ATP–Au electrode. This study suggests that highly sensitive DNA-based biosensors can be developed using relatively low-cost carbonaceous materials.
has issue date	2020-07-13(xsd:dateTime)
bibo:doi	10.1007/s11696-020-01280-5
bibo:pmid	32836707
has license	no-cc
sha1sum (hex)	75e0c6f78c22311296ebb0596e1e8a193e1666d0
schema:url	https://doi.org/10.1007/s11696-020-01280-5
resource representing a document's title	An electrochemical label-free DNA impedimetric sensor with AuNP-modified glass fiber/carbonaceous electrode for the detection of HIV-1 DNA
has PubMed Central identifier	PMC7354876
has PubMed identifier	32836707
schema:publication	Chem Zvesti
resource representing a document's body	covid:75e0c6f78c22311296ebb0596e1e8a193e1666d0#body_text
is schema:about of	named entity 'ssDNA' named entity 'sensor' named entity 'chemistry' named entity 'electrode' named entity 'electrode' named entity 'label-free' named entity 'LABEL-FREE' named entity 'CAPTURE' named entity 'BIOSENSOR' named entity 'USING' named entity 'ATP' named entity 'WORKING RANGE' named entity 'electrode' named entity 'This' named entity 'developed' named entity 'electrodes' named entity 'detection' named entity 'study' named entity 'materials' named entity 'developed' named entity 'conductive' named entity 'DNA probe' named entity 'highly' named entity 'DNA' named entity 'glass fiber' named entity 'coal tar pitch' named entity 'signal to noise ratio' named entity 'gene' named entity 'limit of detection' named entity 'electrochemical' named entity 'electrochemical' named entity 'electrodes' named entity 'self-assembled' named entity 'spectroscopic' named entity 'ATP' named entity 'electrodes' named entity 'gold nanoparticles' named entity 'ATP' named entity 'CTP' named entity 'electrode' named entity 'carbonaceous' named entity 'sensor' named entity 'electrochemical' named entity 'glass fiber' named entity 'carbonized' named entity 'Schematic diagram' named entity 'DNA probes' named entity 'acetonitrile' named entity 'sensor' named entity 'working electrode' named entity 'N-H' named entity 'CTP' named entity 'electrode' named entity 'antiretroviral therapy' named entity 'ATP' named entity 'electrodes' named entity 'Hybridization' named entity 'hybridization' named entity 'viral pathogens' named entity 'ATP' named entity 'electrochemical' named entity 'CTP' named entity 'electrode' named entity 'respiratory syncytial virus' named entity 'electrochemical' named entity 'biosensor' named entity 'electrically conductive' named entity 'Carbonaceous' named entity 'carbon' named entity 'point of care' named entity 'absolute ethanol' named entity 'ATP' named entity 'equivalent circuit' named entity 'ATP' named entity 'hybridization' named entity 'ATP' named entity 'ssDNA' named entity 'electrode' named entity 'electrode' named entity 'CTP'

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