About: Colorimetric Test for Fast Detection of SARS-CoV-2 in Nasal and Throat Swabs

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About: Colorimetric Test for Fast Detection of SARS-CoV-2 in Nasal and Throat Swabs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Colorimetric Test for Fast Detection of SARS-CoV-2 in Nasal and Throat Swabs
Creator	Terracciano, Daniela Bollet-Ti Censi, Sergio Campanile, Raffaele Cennamo, Michele Minopoli, Antonio Portella, Giuseppe Velotta, Raffaele Ventura, Bartolomeo
source	MedRxiv
abstract	Mass testing is fundamental to face the pandemic caused by the coronavirus SARS-CoV-2 discovered at the end of 2019. To this aim, it is necessary to establish reliable, fast and cheap tools to detect viral particles in biological material so to identify the people capable to spread the infection. We demonstrate that a colorimetric biosensor based on gold nanoparticle (AuNP) interaction induced by SARS-CoV-2 lends itself as an outstanding tool for detecting viral particles in nasal and throat swabs. The extinction spectrum of a colloidal solution of multiple viral-target gold nanoparticles - AuNPs functionalized with antibodies targeting three surface proteins of SARS-CoV-2 (spike, envelope and membrane) - is redshifted in few minutes when mixed to a solution containing the viral particle. The optical density of the mixed solution measured at 560 nm was compared to the threshold cycle (Ct) of a Real Time-PCR (gold standard for detecting the presence of viruses) finding that the colorimetric method is able to detect very low viral load with a detection limit approaching that of RT-PCR. Since the method is sensitive to the infecting viral particle rather than to its RNA, the achievements reported here open new perspective not only in the context of the current and possible future pandemics, but also in microbiology as the biosensor proves itself to be a powerful though simple tool for measuring the viral particle concentration.
has issue date	2020-08-18(xsd:dateTime)
bibo:doi	10.1101/2020.08.15.20175489
has license	medrxiv
sha1sum (hex)	75a41b74ebe7abcf7a1d96a8ea0f1cada54dd783
schema:url	https://doi.org/10.1101/2020.08.15.20175489
resource representing a document's title	Colorimetric Test for Fast Detection of SARS-CoV-2 in Nasal and Throat Swabs
resource representing a document's body	covid:75a41b74ebe7abcf7a1d96a8ea0f1cada54dd783#body_text
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