About: Effect of siRNA mediated suppression of signaling lymphocyte activation molecule on replication of peste des petits ruminants virus in vitro

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Effect of siRNA mediated suppression of signaling lymphocyte activation molecule on replication of peste des petits ruminants virus in vitro Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Effect of siRNA mediated suppression of signaling lymphocyte activation molecule on replication of peste des petits ruminants virus in vitro
Creator	Balachandran, C Dhinakar Raj, G Kumar, T Mohanchandra Pawar, Rahul Raja, A
Source	Elsevier; Medline; PMC
abstract	Abstract Signaling lymphocyte activation molecule (SLAM) expression was inhibited in B95a cell line using siRNA and the effect of SLAM inhibition on peste des petits ruminants virus (PPRV) replication and infectivity titre was studied. SLAM suppression was assessed using real-time PCR and flow cytometry to confirm suppression at the m-RNA and protein levels, respectively. Three chemically synthesized siRNAs were transfected individually using oligofectamine into B95a cell line. This resulted in SLAM suppression from 48 to 454-folds, in comparison to the untransfected B95a cell line. When the SLAM suppressed B95a cell line was infected with PPRV, replication was reduced by 12–143-folds and virus titre was reduced from log10 1.09 to 2.28. siRNA 3 showed the most potent inhibition of SLAM expression both at m-RNA and protein levels. This also caused the maximum reduction of virus replication and virus titre. A 100-fold reduction in PPRV titres was seen in anti-SLAM antibody neutralized B95a cell line. This further confirms that SLAM is one of the (co) receptors for PPRV. However, the presence of other putative virus receptor(s) is/are not ruled out.
has issue date	2008-09-30(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2008.04.026
bibo:pmid	18550191
has license	els-covid
sha1sum (hex)	75351f5df204295e001ccdb4ed5af59fc6ea5b39
schema:url	https://doi.org/10.1016/j.virusres.2008.04.026
resource representing a document's title	Effect of siRNA mediated suppression of signaling lymphocyte activation molecule on replication of peste des petits ruminants virus in vitro
has PubMed Central identifier	PMC7127705
has PubMed identifier	18550191
schema:publication	Virus Research
resource representing a document's body	covid:75351f5df204295e001ccdb4ed5af59fc6ea5b39#body_text
is schema:about of	named entity 'SLAM' named entity 'virus' named entity 'siRNA' named entity 'expression' covid:arg/75351f5df204295e001ccdb4ed5af59fc6ea5b39 named entity 'lymphocyte activation' named entity 'inhibited' named entity 'molecule' named entity 'SLAM' named entity 'individually' named entity 'log' named entity 'When' named entity 'inhibition' named entity 'folds' named entity 'cell' named entity 'virus' named entity 'However' named entity 'chemically synthesized' named entity 'reduction' named entity 'potent' named entity 'mediated' named entity 'Signaling lymphocyte activation molecule' named entity 'infectivity' named entity 'virus' named entity 'SLAM' named entity 'cell line' named entity 'chemically synthesized' named entity 'cell line' named entity 'cell line' named entity 'SLAM' named entity 'protein' named entity 'virus replication' named entity 'SLAM' named entity 'SLAM' named entity 'siRNA' named entity 'Vero cells' named entity 'real-time PCR' named entity 'transfected' named entity 'immunoglobulin (Ig) superfamily' named entity 'specific role' named entity 'causative agent' named entity 'siRNA' named entity 'assay' named entity 'virus infection' named entity 'virus' named entity 'siRNA' named entity 'virus' named entity 'cDNA' named entity '100 percent' named entity 'siRNA' named entity 'Paramyxoviridae' named entity 'canine' named entity 'Invitrogen' named entity 'siRNA' named entity 'cell clones' named entity 'siRNA' named entity 'PCR' named entity 'receptor' named entity 'gene' named entity 'pmol' named entity 'negative control' named entity 'cell line' named entity 'sialic acid residues' named entity 'viruses' named entity 'NDV' named entity 'standard curve' named entity 'sub-Saharan' named entity 'siRNA' named entity 'stem cells' named entity 'siRNA' named entity 'siRNA' named entity 'MS Excel' named entity 'transfected' named entity 'PCR' named entity 'siRNA' named entity 'siRNA' named entity 'siRNA'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software